不同抗性水平水稻品种对纹枯病菌毒素的防卫反应与生理差异

左示敏1，# ,陈夕军1,2,# ,陈红旗3 ,徐艳2 ,张家豪2 ,陈羽2 ,陈宗祥1 ,童蕴慧2 ,徐敬友2,* ,潘学彪1,*

doi:10.3969/j.issn.1001-7216.2014.05.013

中国水稻科学 >

2014 , Vol. 28 >Issue 5: 551 - 558

DOI: https://doi.org/10.3969/j.issn.1001-7216.2014.05.013

研究报告

不同抗性水平水稻品种对纹枯病菌毒素的防卫反应与生理差异

展开

¹扬州大学江苏省作物遗传生理重点实验室/江苏省植物功能基因组学重点实验室，江苏扬州 225009; ²扬州大学园艺与植物保护学院，江苏扬州 225009; ³ 中国水稻研究所水稻生物学国家重点实验室，杭州310006；

收稿日期: 2014-05-20

修回日期: 2014-07-20

网络出版日期: 2014-09-10

基金资助

江苏省植物功能基因组学重点实验室项目（K13004）；转基因生物新品种培育重大专项（2014ZX08001003B）；江苏省自主创新资金资助项目\[CX(12)1003\]。

收起

Defense Response and Physiological Difference of Rice Cultivars with Different Sheath Blight Resistance Levels to the Toxins Produced by Rhizoctonia solani

Expand

¹ Key Laboratory of Plant Functional Genomics of Jiangsu Province/Key Laboratory of Crop Genetics and Physiology of Jiangsu Province, Yangzhou University, Yangzhou 225009, China; ²Horticulture and Plant Protection College, Yangzhou University, Yangzhou 225009, China; ³ State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China;

Received date: 2014-05-20

Revised date: 2014-07-20

Online published: 2014-09-10

Fold

摘要

毒素是水稻纹枯病菌（立枯丝核菌Rhizoctonia solani AG1IA）的重要致病因子。研究水稻对毒素的抗性反应、耐受性与对纹枯病菌的抗性间的关系，可为利用毒素快速筛选、鉴定水稻品种的抗病性提供参考依据。研究显示，经乙醚萃取、硅胶柱层析获得的纹枯病菌毒素可显著抑制水稻胚根伸长、诱导典型的纹枯病水渍斑，具有明显生物学活性；以一定浓度的毒素处理水稻植株，可诱导寄主体内过氧化物酶、多酚氧化酶、苯丙氨酸解氨酶和超氧化物歧化酶等防御酶活性升高，且速度或程度与品种抗性水平有关，抗病品种的酶活明显高于感病品种。水稻品种对纹枯病的抗性越强，毒素对其胚根伸长抑制率、细胞膜损伤率和磷素外渗率就越低。毒素活性在长时间光照、高温、强酸、强碱和强氧化性化合物如高锰酸钾中明显下降。

关键词： 水稻; 纹枯病菌; 毒素; 敏感性; 抗性

本文引用格式

左示敏1，# ,陈夕军1,2,# ,陈红旗3 ,徐艳2 ,张家豪2 ,陈羽2 ,陈宗祥1 ,童蕴慧2 ,徐敬友2,* ,潘学彪1,* . 不同抗性水平水稻品种对纹枯病菌毒素的防卫反应与生理差异[J]. 中国水稻科学, 2014 , 28(5) : 551 -558 . DOI: 10.3969/j.issn.1001-7216.2014.05.013

Abstract

Toxins produced by rice sheath blight (SB) pathogen, Rhizoctonia solani AG1IA, are important pathogenicity or virulence factors. It is important to investigate the rice response to the toxin and the correlation between the rice cultivar’s sensitivity to the toxin and susceptibility to the pathogen, which can provide an alternative way for quick selection and identification of resistant cultivars to SB by using SBtoxin. The SBtoxin, extracted by ether and silica gel column chromatography, were biologically active and could inhibit radicle growth of rice seeds and induce typical watersoaked SBlike symptom. After the treatment of SBtoxin at appropriate concentrations, the activities of rice defense enzymes, peroxidase, polyphenol oxidase, phenylalanine ammonia lyase, and superoxide dismutase, were quickly induced with faster rates in SB resistant cultivars than those in susceptible cultivars. The cultivars with higher SB resistance generally presented lower inhibition ratio of radicle growth and fewer ratios of damaged membrane and phosphorous effusion by SBtoxin. The biological activity of SBtoxin dedined under longtime illumination, high temperature and strong acid or alkali treatments, especially under the treatment of oxidative compound such as potassium permanganate.

Key words： Oryza sativa L.; sheath blight pathogen; toxin; sensitivity; resistance

参考文献

\[1\]Lee F N. Rice sheath blight: A major disease. Plant Dis, 1983, 67: 829832.

\[2\]Srinivasachary, Willocquet L, Savary S. Resistance to rice sheath blight (Rhizoctonia solani Kühn) \[(teleomorph: Thanatephorus cucumeris (A.B. Frank) Donk.\] disease: Current status and perspectives. Euphytica, 2010, 178(1): 122.

\[3\]左示敏, 张亚芳, 陈宗祥, 等. 水稻抗纹枯病遗传育种进展. 中国科学: C辑, 2010, 40(11): 10141023.

\[4\]王玲, 黄雯雯, 刘连盟, 等. 对中国南方部分籼型杂交水稻纹枯病抗性的评价. 作物学报, 2011, 37(2): 263270.

\[5\]Jia Y, CorreaVictoria F, McClung A, et al. Rapid determination of rice cultivar responses to the sheath blight pathogen Rhizoctonia solani using a microchamber screening method. Plant Dis, 2007, 91: 485489.

\[6\]王子斌, 左示敏, 李刚, 等. 水稻抗纹枯病苗期快速鉴定技术研究. 植物病理学报, 2008, 39(2): 174182.

\[7\]Park D S, Sayler R J, Hong Y G, et al. A method for inoculation and evaluation of rice sheath blight disease. Plant Dis, 2008, 92: 2529.

\[8\]Sayler R J, Yang Y. Detection and quantification of Rhizoctonia soloani AG1 IA, the rice sheath blight pathogen, in rice using realtime PCR. Plant Dis, 2007, 91(12): 16631668.

\[9\]陈夕军, 徐艳, 童蕴慧, 等. 水稻纹枯病菌毒素致病机理研究. 植物病理学报, 2009, 39(4): 439443.

\[10\]Vidhyasekaran P, Ponmalar T R, Samiyappan R, et al. Hostspecific toxin production by Rhizoctonia solani, the rice sheath blight pathogen. Phytopathology, 1997, 87(12): 12581263.

\[11\]石凤梅. 植物病原真菌毒素的研究进展. 黑龙江农业科学, 2006(2): 7073.

\[12\]魏松红, 刘文合, 俞孕珍, 等. 稻瘟病菌毒素对水稻愈伤组织防御酶系的诱导. 沈阳农业大学学报, 2000, 31(4): 328330.

\[13\]于莉. 黑斑毒素的提取及对向日葵超微结构和防御酶系的影响. 植物病理学报, 2000, 30(1): 96.

\[14\]Brooks S A. Sensitivity to a phytotoxin from Rhizoctonia solani correlates with sheath blight susceptibility in rice. Phytopathology, 2007, 10(97): 12071212.

\[15\]敖世恩, 杨媚, 周而勋, 等. 水稻抗纹枯病突变体的离体筛选. 华南农业大学学报, 2006, 27(1): 4750.

\[16\]陈夕军, 潘存红, 孟令军, 等. 水稻纹枯病菌毒素提纯及其组分分析. 扬州大学学报: 农业与生命科学版, 2011, 32(1): 4449.

\[17\]徐敬友, 张华东, 张红, 等. 立枯丝核菌毒素的产生及与致病力的关系. 扬州大学学报, 2004, 25(2): 6263.

\[18\]汤章城, 王国强, 史益敏, 等. 现代植物生理学实验指南. 北京: 科学出版社, 1999.

\[19\]李靖, 利容千, 袁文静. 黄瓜感染霜霉病菌叶片中一些酶活性的变化. 植物病理学报, 2001, 31(1): 9192.

\[20\]周琪. 植物生理实验指导. 北京: 中国农业大学出版社, 2001.

\[21\]陈旭升, 林玲, 狄佳春, 等. 黄萎病菌毒素诱导棉苗子叶组织磷素外渗规律初探. 江西棉花, 2003, 25(5): 710.

\[22\]张国良, 戴其根, 张洪程, 等. 硅肥和接种纹枯病菌对水稻膜脂过氧化和防御酶活性的影响. 扬州大学学报, 2006, 27: 4953.

\[23\]左示敏, 张玉梅, 薛芗, 等. 水稻新种质YSBR1抗纹枯病机制的初步研究. 中国水稻科学, 2014, 28(2): 132140.

\[24\]童蕴慧, 徐敬友, 潘学彪, 等. 水稻植株对纹枯病菌侵染反应及其机理的初步研究. 江苏农业研究, 2000, 21(4): 4547.

\[25\]Zhang G L, Cui Y X, Ding X W, et al. Stimulation of phenolic metabolism by silicon contributes to rice resistance to sheath blight. J Plant Nutr Soil Sci, 2013, 176: 118124.

\[26\]左示敏，张亚芳，殷跃军，等. 田间水稻纹枯病抗性鉴定体系的确立与完善. 扬州大学学报: 农业与生命科学版, 2006, 27(4): 5761.

\[27\]左示敏, 王子斌, 陈夕军, 等. 水稻纹枯病改良新抗源YSBR1的抗性评价. 作物学报, 2009, 35(4): 608614.

\[28\]Shanmugam V, Sriram S, Babu S, et al. Purification and characterization of an extracellular alphaglucosidase protein from Trichoderma viride which degrades a phytotoxin associated with sheath blight disease in rice. Appl Microbiol, 2001, 90(3): 320329.

\[29\]Sriram S, Raguchander T, Babu S, et al. Inactivation of phytotoxin produced by the rice sheath blight pathogen Rhizoctonia solani. Can J Microbiol, 2000, 46(6): 520524.

Options

文章导航

模态框（Modal）标题

摘要

本文引用格式

Abstract

参考文献