研究报告

沈农系列水稻高产品种及其衍生系的遗传结构分析

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  • 沈阳农业大学 水稻研究所/农业部东北水稻生物学与遗传育种重点实验室/北方超级粳稻育种教育部重点实验室,沈阳 110866;

收稿日期: 2014-01-19

  修回日期: 2014-03-18

  网络出版日期: 2014-09-10

基金资助

辽宁省高等学校优秀人才支持计划资助项目(UQ2013075)。

Structure Analysis of Highyielding Rice Varieties and Their Derived Lines Bred by Shenyang Agricultural University

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  • Rice Research Institute/Key Laboratory of Rice Biology and Breeding, Ministry of Agriculture/ Key Laboratory of Northern japonica Super Rice Breeding, Ministry of Education, Shenyang Agricultural University, Shenyang 110866, China;

Received date: 2014-01-19

  Revised date: 2014-03-18

  Online published: 2014-09-10

摘要

以沈农265、沈农606、沈农9741、千重浪1号、沈农9903、沈农9816等6个高产品种及其衍生的570份品系为材料,通过对DEP1、GS5、qSW5、Gn1a、qGW8、GS3和GW2等 7个功能基因位点的检测来分析这些已经克隆的控制产量性状的基因在辽宁水稻育种中的应用情况;同时,利用籼粳特异性的InDel标记分析籼稻血缘含量对北方高产优质粳稻育种的贡献。结果表明,除了GW2外,其他6个功能基因位点均检测到等位基因的变异。576份材料中,DEP1、qSW5和qGW8 在沈农系列粳稻品种中被广泛利用。检测到467份材料含有DEP1基因,占总数的81.9%;550份材料含有qSW5基因,占965%;351份材料含有qGW8,占61.6%;而GS5、Gn1a以及GS3在育种中被利用得较少,仅在少数品系中被利用,比例分别为5.4%、0.7%和1.1%。籼粳特异性标记分析表明,54.0%的参试材料在32个位点上有一定籼型血缘引入,且第5染色体上存在的籼型位点较多,占该染色体的20.8%。

本文引用格式

杨贤莉,王嘉宇*,刘丹,张玲,李晓楠,吕国依 . 沈农系列水稻高产品种及其衍生系的遗传结构分析[J]. 中国水稻科学, 2014 , 28(5) : 496 -502 . DOI: 10.3969/j.issn.1001-7216.2014.05.007

Abstract

Six highyield varieties (Shennong 265, Shennong 606, Shennong 9741, Qianchonglang 1, Shennong 9903, Shennong 9816) and 570 inbred lines were used as materials to analyze the utilization of seven cloned yieldrelated functional gene (DEP1, GS5, qSW5, Gn1a, qGW8, GS3 and  GW2) and the contribution of indica blood in the japonica rice breeding of Liaoning Province. The results showed that allelic variations were detected from all of the functional gene loci except for GW2. The loci of DEP1, qSW5 and qGW8 were widely used in japonica rice breeding by the breeders at Shenyang Agricultural University. In the 570 inbred lines, 467 lines (82.13% of the lines) contained DEP1, 550 lines (96.5% of the lines) contained qSW5, and 351 lines (61.6% of the lines) contained qGW8. The functional alleles of GS5, Gn1a and GS3 were also present in a small number of lines, and the rates were 5.44%, 070% and 1.05%, respectively. The results of the analysis with 32 indicajaponica specific markers showed that indicajaponica differentiation on these sites existed in 54.0% of the lines. Most of the indica genetic loci were located on chromosome 5, with a percentage of 20.8%.

参考文献

\[1\]刘迪, 李红宇, 孙健, 等. 应用SSR分子标记评价不同年代东北三省粳稻基因组遗传构成. 黑龙江农业科学, 2011(7): 16.

\[2\]张立娜, 曹桂兰, 韩龙植. 利用SSR标记揭示中国粳稻地方品种遗传多样性. 中国农业科学, 2012, 45(3): 405413.

\[3\]杨守仁, 张龙步, 陈温福, 等. 水稻超高产育种的理论和方法. 中国水稻科学, 1996, 10(2): 115120.

\[4\]Huang X Z, Qian Q,  Liu H Y,  et al.  Natural variation at the DEP1 locus enhances grain yield in rice.  Nat Genet,  2009, 41(4): 494497.

\[5\]Li Y B, Fan C C, Xing Y Z, et al.  Natural variation in GS5 plays an important role in regulating grain size and yield in rice.  Nat Genet,  2011, 43(12): 12661269.

\[6\] Shomura A,  Izawa T,  Ebana K, et al. Deletion in a gene associated with grain size increased yields during rice domestication. Nat Genet,  2008, 40(8): 10231028.

\[7\]Ashikari M, Sakakibara H,  Lin S, et al. Cytokinin oxidase regulates rice grain production. Science,  2005, 309(5735): 741745.

\[8\]Wang S K, Wu K,  Yuan Q B, et al. Control of grain size, shape and quality by OsSPL16 in rice.  Nat Genet, 2012, 44(8): 950954.

\[9\]TakanoKai N, Doi K,  Yoshimura A.  GS3 participates in stigma exsertion as well as seed length in rice.  Breeding Sci, 2011,  61(3): 244250.

\[10\] Song X J, Huang W, Shi M, et al. A QTL for rice grain width and weight encodes a previously unknown RINGtype E3 Ubiquitin ligase.  Nat Genet,  2007, 39(5): 623630.

\[11\]刘丹, 王嘉宇, 孙健, 等. 利用InDel和SSILP标记分析北方粳型超级稻的遗传组成. 中国水稻科学, 2014, 28(2): 148154.

\[12\]Doyle J J, Doyle J L.  A rapid DNA isolation procedure for small quantities of fresh leaf tissue.   Phytochem Bull,  1987, 19: 1115. 

\[13\]Yan C J, Yan S, Yang Y C, et al. Development of genetagged markers for quantitative trait loci underlying rice yield components.  Euphytica,  2009, 169(2): 215226.

\[14\]Sun J, Liu D, Wang J Y, et al.  The contribution of intersubspecic hybridization to the breeding of superhighyielding japonica rice in northeast China.  Theor Appl Genet,  2012, 125(6): 11491157.

\[15\] Lu B R, Cai  X X, Jin X. Efficient indica and japonica rice identification based on the InDel molecular method: Its implication in rice breeding and evolutionary research.  Progr Nat Sci,  2009, 19(10): 12411252.

\[16\]杨守仁. 水稻超高产育种新动向—理想株型与优势利用相结合. 沈阳农业大学学报, 1987, 18(1): 15.

\[17\]Nei M.  Genetic distance between populations.  Am Nat,  1972, 106: 283292.

\[18\] Yamasaki M, Ideta O.  Population structure in Japanese rice population.  Breeding Sci, 2013, 63: 4957. 

\[19\]Choudhury B, Khan M L, Dayanandan S. Genetic structure and diversity of indigenous rice(Oryza sativa) varieties in the Eastern Himalayan region of Northeast India.  SpringerPlus, 2013, 2(1): 110.

\[20\]刘化龙, 郑洪亮, 赵宏伟, 等. 东北粳稻群体结构和连锁不平衡分析. 分子植物育种, 2013, 11(5): 494501.

\[21\]贾宝艳, 王伯伦, 关荣霞, 等. 辽宁不同株型水稻品种的RAPD分析. 作物学报, 2005, 31(7): 952955.

\[22\]姜树坤, 王政海, 钟鸣, 等. 辽宁省近15年的部分水稻主栽品种的简单重复序列(SSR)多态性分析. 植物生理学通讯, 2007, 43(1): 6972.

\[23\]高虹, 李飞飞, 吕国依, 等. 籼粳稻杂交对中国东北粳稻品质的影响. 作物学报, 2013, 39(10): 18061813.
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