水稻千粒重基因TGW6功能标记的开发与利用

王军1 ,杨杰1 ,徐祥2 ,朱金燕1 ,范方军1 ,李文奇1 ,王芳权1 ,仲维功1,*

doi:10.3969/j.issn.1001-7216.2014.05.004

中国水稻科学 >

2014 , Vol. 28 >Issue 5: 473 - 478

DOI: https://doi.org/10.3969/j.issn.1001-7216.2014.05.004

研究报告

水稻千粒重基因TGW6功能标记的开发与利用

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¹江苏省农业科学院粮食作物研究所/国家水稻改良中心南京分中心，南京210014；²扬州大学生物技术学院，江苏扬州 225009；

收稿日期: 2014-04-09

修回日期: 2014-05-04

网络出版日期: 2014-09-10

基金资助

国家自然科学基金资助项目(31101131)；国家科技支撑计划重大项目(2011BAD16B03)；江苏省农业自主创新资金资助项目\[CX(12)1003\]；江苏省自然科学基金资助项目（BK20130725）；江苏省科技支撑计划资助项目(BE2012309)。

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Development and Application of a Functional Marker for Grain Weight Gene TGW6 in Rice

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¹Institute of Food Crops, Jiangsu Academy of Agricultural Sciences/Nanjing Branch of Chinese National Center for Rice Improvement, Nanjing 210014, China；²College of Bioscience and Biotechnology, Yangzhou University, Yangzhou 225009,China;

Received date: 2014-04-09

Revised date: 2014-05-04

Online published: 2014-09-10

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摘要

水稻粒重是影响产量及品质的重要性状之一，TGW6是能显著增加千粒重同时不影响稻米品质的粒重基因。为提高千粒重基因TGW6在育种中的选择效率，在前人克隆的基础上，根据TGW6与其等位基因tgw6在功能区域存在的单碱基缺失，设计和筛选出TGW6基因的功能标记CAPs61，对CAPs61扩增的PCR产物测序验证，说明CAPs61可以准确鉴定出TGW6的不同基因型。利用该标记对不同来源的43份籼稻资源、江苏2007-2013年审定的65份粳稻品种和太湖流域179份粳稻地方资源进行了TGW6基因型检测，筛选到携带TGW6基因的籼稻资源14份，244份粳型资源均不携带TGW6基因，这也说明TGW6主要分布在籼型水稻资源中，在粳型资源中几乎不存在。

关键词： 水稻; 千粒重; TGW6; 功能标记

本文引用格式

王军1 ,杨杰1 ,徐祥2 ,朱金燕1 ,范方军1 ,李文奇1 ,王芳权1 ,仲维功1,* . 水稻千粒重基因TGW6功能标记的开发与利用[J]. 中国水稻科学, 2014 , 28(5) : 473 -478 . DOI: 10.3969/j.issn.1001-7216.2014.05.004

Abstract

Grain weight is one of the most important factors in grain yield and grain quality. TGW6 was reported to significantly enhance thousandgrain weight without negative effect on grain quality. To improve the selection efficiency of grain weight gene TGW6 in breeding, the marker CAPs61 based on the single nucleotide deletion in the functional region of alleles TGW6/tgw6 was selected and furtherly confirmed to be a gene based marker via sequencing CAPs61 PCR products. CAPs61 was employed to genotype 43 indica rice varieties, 65 japonica varieties certificated in Jiangsu Province from 2007 to 2013, and 179 local japonica cultivars from Taihu Basin. The results showed that there were only 14 indica varieties carrying TGW6 gene, while TGW6 gene was not detected in 244 japonica rice samples, indicating that TGW6 mainly exists in indica germplasm, and is rarely found in japonica rice varieties.

Key words： rice; thousandgrain weight; TGW6; functional marker

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