Specific primers were designed for tetraprimer amplification refractory mutation system PCR (ARMSPCR) method according to the single nucleodide mutation in gene p/tms121. Eleven twoline sterile lines and two normal varieties were analyzed by this method. The results could clearly distinguish the genotype of p/tms121, which was completely consistent with the result of CAPs markerbased analysis. Therefore, as a lowcost, simple and reliable technique, this method could be widely used to the p/tms121 detection and molecular markerassisted breeding in rice.
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