为了阐明不同BT型杂交粳稻组合间育性差异的原因，利用豫粳6号A、9201A及其相应保持系与恢复系TR2604、闵120来配制遗传组合就BT型不育细胞质育性恢复遗传进行研究。根据9201A//9201B/闵120、9201A//9201B/TR2604及豫粳6号A//豫粳6号B/闵120三个群体中植株的育性分离，推断 9201A、豫粳6号A的育性恢复均由1对恢复基因控制。基因定位结果表明，恢复系闵120中的恢复基因位于第10染色体上18772374－18967996bp间约195 kb物理区间内，TR2604中的恢复基因位于第10染色体18 817 631－18 967 996 bp间约150 kb物理区间内。根据已克隆Rf1基因的信息可知两恢复系带有的恢复基因均为Rf1a。豫粳6号A/TR2604//TR2604 BC1F2群体中植株的Rf1a位点基因型与育性分析结果表明TR2604对豫粳6号A育性恢复由Rf1a控制，豫粳6号A/TR2604不育由双亲间的不亲和性造成。
张宏根#,钱凯#,孙一标,裴艳,封智蔷,刘晓宇,汤述翥*,梁国华,顾铭洪
. BT型三系杂交粳稻不同组合间育性差异原因初探[J]. 中国水稻科学, 2014
, 28(4)
: 377
-383
.
DOI: 10.3969/j.issn.1001-7216.2014.04.006
In order to understand the genetic basis of spikelet fertility differences between BTtype threeline japonica hybrids, the CMS lines Yujing 6A, 9201A and their corresponding maintainer lines, and the restorer lines Min 120, TR2604 were used to develop the genetic populations for studying the genetic characteristics of BTtype CMS in different hybrid combinations. According to fertility segregation of the plants from the populations of 9201A//9201B/Min 120, Yujing 6A//Yujing 6B/Min 120 and 9201A//9201B/ TR2604, it was concluded that the fertility restoration of 9201A and Yujing 6A was controlled by one fertility restoration(Rf) gene. Then, the Rf genes in Min120, TR2604 were mapped between 18 772 374－18 967 996 bp with the physical distance of 195 kb and 18 817 631－18 967 996 bp with the physical distance of 150 kb on chromosome 10, respectively, indicating that the Rf genes in Min 120, TR2604 might be Rf1a according to the mapping results of Rf1 in former studies. The fertility restoration of Yujing 6A in the population derived from Yujing 6A and TR2604 was also controlled by Rf1a identified by the genotype and phenotype of plants in Yujing 6A/TR2604//TR2604 BC1F2 population, so the sterility of Yujing 6A/TR2604 was induced by hybrid incompatibility between the two parents.
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