江苏省杂草稻Rc基因的单体型分析

李潇艳,强胜,宋小玲,蔡堃,戴伟民*

doi:10.3969/j.issn.1001-7216.2014.03.010

中国水稻科学 >

2014 , Vol. 28 >Issue 3: 304 - 313

DOI: https://doi.org/10.3969/j.issn.1001-7216.2014.03.010

研究报告

江苏省杂草稻Rc基因的单体型分析

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南京农业大学生命科学学院杂草研究室，南京 210095；

收稿日期: 2013-07-05

修回日期: 2013-11-09

网络出版日期: 2014-05-10

基金资助

转基因生物新品种培育科技重大专项（2013ZX08011001, 2009ZX08012020B）；国家自然科学基金资助项目（30800604）；公益性行业（农业）科研专项（201303022）；国家基础科学人才培养基金南京农业大学生物学理科基地科研训练及科研能力提高项目（J1210056）；江苏省科技支撑计划资助项目（BE2011353）。

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Haplotype Analysis of Rc Gene for Weedy Rice in Jiangsu Province

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Weed Research Laboratory, College of Life Sciences,Nanjing Agriculture University, Nanjing 210095, China;

Received date: 2013-07-05

Revised date: 2013-11-09

Online published: 2014-05-10

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摘要

测定了来自江苏省10个市的杂草稻样品的Rc基因序列（全长6.4 kb），并整理现有文献中全球166份稻属的Rc基因序列。序列共线性分析表明，江苏杂草稻Rc基因等位基因类型全部为Rc野生型。核苷酸多态性和单倍型分析表明，10份江苏杂草稻Rc基因的核苷酸多态性（π=0.19）和分离位点比率（θw =0.28）均高于56份美国杂草稻（π=0.09；θw =0.07）。单倍型的进化网络和系统进化树分析表明，江苏杂草稻Rc基因并非直接来源于普通白色栽培稻回复突变。此外，发现连云港穞稻和安徽塘稻的Rc基因序列与栽培稻相似，而与江苏目前正在发生危害的杂草稻Rc基因距离较远。

关键词： 杂草稻; 红色果皮; Rc基因; 穞稻; 塘稻; 单倍型分析; 核苷酸多态性

本文引用格式

李潇艳,强胜,宋小玲,蔡堃,戴伟民* . 江苏省杂草稻Rc基因的单体型分析[J]. 中国水稻科学, 2014 , 28(3) : 304 -313 . DOI: 10.3969/j.issn.1001-7216.2014.03.010

Abstract

Rc gene(about 6.4 kb) of 10 weedy rice samples from 10 cities in Jiangsu Province was sequenced and 166 Rc fulllength sequences of Oryza genus in literatures were reviewed. Colinear sequence analysis showed that Rc allele of Jiangsu weedy rice was wild genotype. Nucleotide polymorphism and haplotype analysis showed that Rc nucleotide polymorphism (π=0.19) and separation loci ratio (θw=0.28) of 10 weedy rice samples in Jiangsu Province were higher than that of 56 weedy rice samples from the United States (π=0.09; θw=0.07). Haplotype network and evolutionary phylogenetic analysis showed that Rc gene of weedy rice in Jiangsu was not directly derived from the revertant mutation of white cultivar. In addition, Rc gene sequences of Ludao from Lianyungang,Jiangsu and Tangdao from Anhui were similar to that of cultivated rice, while genetically distant from weedy rice samples which has been leading to serious infestation in Jiangsu Province.

Key words： weedy rice; red pericarp; Rc gene; Ludao; Tangdao; haplotype analysis; nucleotide polymorphism

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