褐飞虱体内细菌群落的PCRDGGE分析

徐红星1,2,郑许松2,杨亚军2,王新2,傅强3,叶恭银1,*,吕仲贤2,*

doi:10.3969/j.issn.1001-7216.2014.02.015

中国水稻科学 >

2014 , Vol. 28 >Issue 2: 217 - 222

DOI: https://doi.org/10.3969/j.issn.1001-7216.2014.02.015

研究简报

褐飞虱体内细菌群落的PCRDGGE分析

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¹浙江大学昆虫科学研究所, 杭州 310029；²浙江省农业科学院植物保护与微生物研究所, 杭州 310021; ³中国水稻研究所，杭州 310006；

收稿日期: 2013-11-07

修回日期: 2014-01-23

网络出版日期: 2014-03-10

基金资助

国家973计划资助项目（2010CB126202）；浙江省优先主题农业项目（2011C12022）。

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PCRDGGE Analysis of the Bacterial Community in Different Populations of Rice Brown Planthopper, Nilaparvata lugens Stl

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¹ Institute of Insect Science, Zhejiang University Hangzhou 310058, China; ² Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China; ³China National Rice Research Institute, Hangzhou 310006, China;

Received date: 2013-11-07

Revised date: 2014-01-23

Online published: 2014-03-10

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摘要

应用变性梯度凝胶电泳（denatured gradient gel electrophoresis，DGGE）分析了褐飞虱体内细菌的群落结构。结果表明，褐飞虱1龄～5龄若虫体内细菌的菌群结构随其生长发育而异，而羽化后的成虫与1龄若虫的菌群结构具有较高的相似性。根据DGGE聚类分析结果，9个地理种群褐飞虱体内细菌可分为3类，菲律宾单独一组，泰国与我国的海南、云南和浙江种群为一组，越南与我国的广西、湖南和江西种群为一组。取食感虫品种的TN1种群褐飞虱体内细菌的群落结构相似，与取食抗虫品种的ASD7种群和Mudgo种群之间具有较明显的差异，但取食不同抗虫水稻品种的褐飞虱还未能得到有效区分。

关键词： 变性梯度凝胶电泳; 褐飞虱; 菌群结构; 地理种群; 致害性

本文引用格式

徐红星1,2,郑许松2,杨亚军2,王新2,傅强3,叶恭银1,*,吕仲贤2,* . 褐飞虱体内细菌群落的PCRDGGE分析[J]. 中国水稻科学, 2014 , 28(2) : 217 -222 . DOI: 10.3969/j.issn.1001-7216.2014.02.015

Abstract

The bacterial community structure in different virulent populations of brown planthopper (BPH), Nilaparvata lugens (Stl), was analyzed by denatured gradient gel electrophoresis (DGGE). The results showed that the bacterial community in BPH nymph from the 1st to the 5th instars varied with nymphal growth and development, and the bacterial community in the 1st instar BPH nymph was similar to that in adults. Nine geographic BPH populations were obviously divided into three groups according to the cluster analysis based on DGGE fingerprint. The first group came from the Philippines, the second from Thailand and the provinces of Hainan, Yunnan and Zhejiang of China, and the third from Vietnam and the provinces of Guangxi, Hunan, and Jiangxi of China. BPH populations on Mudgo (with resistant gene Bph1) and ASD7 (with resistant gene bph2) have similar bacterial community, while differed to that fed on susceptible rice variety, TN1.

Key words： denatured gradient gel electrophoresis (DGGE); brown planthopper (BPH); Nilaparvata lugens; bacterial community; geographic population; virulent to resistant varieties

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