稻瘟病抗病基因Pi-kh功能标记的开发及江苏粳稻品种中Pi-kh的变异

王军,杨杰,朱金燕,范方军,李文奇,王芳权,黄转运,仲维功*

doi:10.3969/j.issn.1001-7216.2014.02.005

中国水稻科学 >

2014 , Vol. 28 >Issue 2: 141 - 147

DOI: https://doi.org/10.3969/j.issn.1001-7216.2014.02.005

研究报告

稻瘟病抗病基因Pi-k^h功能标记的开发及江苏粳稻品种中Pi-k^h的变异

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江苏省农业科学院粮食作物研究所/国家水稻改良中心南京分中心，南京210014;

收稿日期: 2013-09-02

修回日期: 2013-10-12

网络出版日期: 2014-03-10

基金资助

国家科技支撑计划重大项目 (2011BAD16B03)；国家自然科学基金资助项目(31101131)；江苏省农业自主创新资金资助项目\[CX(12)1003\]；江苏省科技支撑计划资助项目(BE2012309)；江苏省自然科学基金资助项目(BK2011666)。

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Development of a Functional Marker for Rice Blast Resistance Gene Pikh and Natural Variation at Pikh Locus in japonica Rice in Jiangsu Province， China

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Institute of Food Crops, Jiangsu Academy of Agricultural Sciences / Nanjing Branch of Chinese National Center for Rice Improvement, Nanjing 210014, China；

Received date: 2013-09-02

Revised date: 2013-10-12

Online published: 2014-03-10

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摘要

稻瘟病抗病基因Pikh是对稻瘟病菌具有广谱抗性的一个重要基因。根据Pikh感病基因和抗病基因序列存在143bp 插入的特征，设计了InDel 标记FM143。Tetep具有抗病基因Pikh，分子标记FM143 PCR 扩增带型为非插入带型，而不携带Pikh的材料的带型为插入带型。利用该标记对Tetep与宁9108的F2 群体进行分析，结果表明该标记能准确区分Pikh、Pikh纯合及杂合基因型，标记基因型符合1∶2∶1比例，没有发生偏分离现象。利用该标记对65份江苏省历年来大面积推广的粳稻品种和64 份粳稻品系进行检测，筛选出具有非插入带型的粳稻品种19份、粳稻品系22份。进一步对江苏省粳稻品种中Pikh位点进行测序，发现江苏粳稻品种（系）Pikh基因座位与Tetep和已经登录的Pikh序列相比在102 bp处存在一个单碱基的变异（由G突变为C），使得谷氨酸变异为天门冬氨酸，且该变异高度保守。将江苏省粳稻品种中发现的Pikh位点的等位基因暂定名为PikhJSJ。

关键词： 水稻; 稻瘟病; Pikh; 功能标记; PikhJSJ

本文引用格式

王军,杨杰,朱金燕,范方军,李文奇,王芳权,黄转运,仲维功* . 稻瘟病抗病基因Pi-k^h功能标记的开发及江苏粳稻品种中Pi-k^h的变异[J]. 中国水稻科学, 2014 , 28(2) : 141 -147 . DOI: 10.3969/j.issn.1001-7216.2014.02.005

Abstract

Pikhis a vital non racespecific resistance gene for rice blast. In this study, one InDel marker, FM143 was developed based on the 143 bp insertion between the susceptible and resistant Pikh alleles. Previous studies showed that Tetep carried resistant Pikh allele and had the noneinsertion genotype for PCR by FM143. F2 population, derived from Tetep and Ning 9108, were detected by marker FM143 and the result indicated that FM143 could effectively identify Pikh/Pikh, Pikh/pikh and pikh/pikh genotypes with the expected segregation ratio 1∶2∶1. Sixtyfive japonica varieties widely grown in Jiangsu Province over years and sixtyfour japonica lines were genotyped by FM143. Among them, 19 japonica varieties and 22 japonica lines showed noneinsertion genotype. Further sequencing at the target Pikh locus for these noneinsertion genotype showed that there was one highly conserved singlebase mutation (G→C) at 102 bp location compared with the whole target gene sequence in Tetep and public Pikh GeneBank leading to the changes from glutamic acid to asparaginic acid. This new allele in Jiangsu japonica rice was termed as PikhJSJ.

Key words： rice; rice blast; Pikh; functional marker; PikhJSJ

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