粳稻苗期长根突变体<em>lr1</em>的形态鉴定和基因定位

廖莉1 ,章骁劼1 ,吴建国2 ,石春海1, *

doi:10.3969/j.issn.1001-7216.2014.02.002

中国水稻科学 >

2014 , Vol. 28 >Issue 2: 119 - 124

DOI: https://doi.org/10.3969/j.issn.1001-7216.2014.02.002

研究报告

粳稻苗期长根突变体lr1的形态鉴定和基因定位

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¹浙江大学农业与生物技术学院农学系，杭州 310058；²浙江农林大学农业与食品科学学院，浙江临安 311300；

收稿日期: 2013-10-16

修回日期: 2013-10-22

网络出版日期: 2014-03-10

基金资助

浙江省重大科技攻关专项（2012C129012）；浙江省自然科学基金重点项目（Z3100089）；浙江省科技厅创新团队项目（2010R50024）；教育部创新团队项目（IRT1185）。

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Morphological Identification and Gene Mapping of a Seedling Long Root Mutant (lr1) in japonica Rice

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¹Agronomy Department, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China; ²College of Agriculture and Food Science, Zhejiang A&F University, Lin’an 311300, China;

Received date: 2013-10-16

Revised date: 2013-10-22

Online published: 2014-03-10

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摘要

通过甲基磺酸乙酯诱导粳稻品种日本晴，筛选到一个能稳定遗传的苗期长根突变体 lr1 （long root 1）。在发芽后2、4、6、10和15 d， lr1 的主根长度分别比日本晴野生型主根长1.52、1.93、3.93、6.81和6.77 cm，根冠比也显著大于野生型。遗传分析结果表明该基因受一对隐性单基因控制。利用SSR分子标记将该突变基因 LR1 定位于第10染色体长臂上，位于标记RM304和RM7300之间，遗传距离分别为20 cM和3.5 cM。

关键词： 水稻; 长根; 形态鉴定; 基因定位

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廖莉1 ,章骁劼1 ,吴建国2 ,石春海1, * . 粳稻苗期长根突变体lr1的形态鉴定和基因定位[J]. 中国水稻科学, 2014 , 28(2) : 119 -124 . DOI: 10.3969/j.issn.1001-7216.2014.02.002

Abstract

A seedling long root mutant named lr1 (long root 1) was isolated from rice cultivar Nipponbare by mutagenesis with ethyl methanesulfonate (EMS) and the long root trait can be stably inherited. The main root in lr1 mutant was 1.52, 1.93, 3.93, 6.81 and 6.77 cm longer than those of wild type at 2, 4, 6, 10 and 15day after germination, respectively, and the rootshoot ratio of lr1 mutant was also significantly greater than that in the seedling of Nipponbare. Genetic analysis indicated that the seedling long root mutant phenotype was controlled by a single recessive gene. The LR1 gene was located between the SSR markers RM304 and RM7300 on chromosome 10, and the genetic distance is 20 cM and 3.5 cM, respectively.

Key words： rice; long root; morphological identification; gene mapping

参考文献

\[1\]Yoan C, Christophe P, Brigitte C, et al. Genetic control of root development in rice, the model cereal. Trends Plant Sci, 2010, 4: 219226.

\[2\]Xu C M, Wang D Y, Chen S, et al. Effects of aeration on root physiology and nitrogen metabolism in rice. Rice Sci, 2013, 20(2): 148153.

\[3\]黄沆, 陈光辉. 水稻根系育种的研究现状及展望. 湖南农业大学学报:自然科学版, 2009, 35(S1): 3539.

\[4\]宁永强, 丁沃娜, 朱世华, 等. 水稻短根突变体ksr1的遗传分析和基因定位. 中国水稻科学, 2010, 24(6): 652654.

\[5\]Cheng J C, Seeley K A, Sung Z R. RML1 and RML2, Arabidopsis genes required for cell proliferation at the root tip. Plant Physiol, 1995, 107: 365376.

\[6\]Vernoux T, Wilson R C, Seeley K A, et al. The root meristemless1/cadmium sensitive2 gene defines a glutathionedependent pathway involved in initiation and maintenance of cell division during postembryonic root development.Plant Cell, 2000, 12: 97109.

\[7\]Baskin T I, Betzner A S, Hoggart R, et al. Root morphology mutants in Arabidopsis thaliana. Aust J Plant Physiol, 1992, 19: 427437.

\[8\]Cano D A, Metzlaff K, Bevan M W. The eli1 mutation reveals a link between cell expansion and secondary cell wall formarion in Arabidopsis thaliana. Development, 2000, 127: 33953405.

\[9\]Fagard M, Desnos T, Desprez T, et al. PROCUSTE1 encodes a cellulose synthase required for normal cell elongation specifically in roots and darkgrown hypocotyls of Arabidopsis. Plant Cell, 2000, 12: 24092424.

\[10\]Frank H, Woong J P, Gunter H F. Cooperative action of SLR1 and SLR2 is required for lateral rootspecific cell elongation in maize. Plant Physiol, 2001, 125： 15291539.

\[11\]Roberto T, Maria C S, Pierangelo L, et al. Identification of QTLs for root characteristics in maize grown in hydroponics and analysis of their overlap with QTLs for grain yield in the field at two water regimes.Plant Mol Biol, 2002, 48: 697712.

\[12\]周晓果, 景蕊莲, 郝转芳, 等. 小麦幼苗根系性状的 QTL 分析. 中国农业科学, 2005, 38(10): 19511957.

\[13\]班超, 张晓玲, 穆平. 水稻根系性状QTL的整合、分类和真实性分析. 中国农学通报, 2009, 25(19): 2025.

\[14\]Yoshiaki I, Masami M, Yasuo N, et al. Characterization of rice mutants deficient in the formation of crown roots.Breed Sci, 2001, 51: 123129.

\[15\]Inukai Y, Miwa M, Nagato Y, et al. RRL1, RRL2 and CRL2 loci regulating root elongation in rice. Breed Sci, 2001, 51: 231239.

\[16\]Chul M K, Sung H P, Byong I J, et al. OsCSLD1, a cellulose synthaselike D1 gene, is required for root hair morphogenesis in rice. Plant Physiol, 2007, 3: 12201230.

\[17\]Jia L Q, Wu Z C, Hao X, et al. Identification of a novel mito chondrial protein, short postembryonic roots 1(SPR1), involved in root development and iron homeostasis in Oryza sativa. New Phytol, 2011, 189: 843855.

\[18\]丁沃娜, 吴晶, 罗丽丽, 等. 水稻短根毛突变体ksrh1的遗传分析和基因定位. 中国水稻科学, 2012, 26(1): 14.

\[19\]罗丽丽, 史俊颖, 项显波, 等.水稻短根相关基因OsKSR2的定位. 作物学报, 2012, 38(3): 429435.

\[20\]Yi K K, Wu Z, Zhou J, et al. OsPTF1, a novel transcription factor involved in tolerance to phosphate starvation in rice. Plant Physiol, 2005, 8(138): 20872096.

\[21\]Yang Y Q, Mu P, Zhang H L, et al. Mapping QTLs of root morphological traits at different growth stages in rice. Genetica, 2008, 133: 187200.

\[22\]Champoux M C, Wang G, Sarkarung S, et al. Locating genes associated with root morphology and drought avoidance in rice via linkage to molecular markers. Theor Appl Genet, 1995, 90: 969981.

\[23\]Nguyen H T, Babu R C, Blum A. Breeding for drought resistance in rice: Physiology and molecular genetics consideration.Crop Sci, 1997, 37: 14261434.

\[24\]Price A, Courtois B. Mapping QTLs associated with drought resistance in rice: Progress, problems and prospects.Plant Growth Regul, 1999, 29: 123133.

\[25\]Yoshiaki I, Masami M, Yasuo N, et al. RRL1, RRL2 and CRL2 loci regulation root elongation in rice. Breed Sci, 2001, 51: 231239.

\[26\]Rogers S O, Bendich A J. Extraction of DNA from milligram amounts of fresh, herbarium and mummified plant tissues.Plant Mol Biol, 1985, 5(2): 6976.

\[27\]Michelmore R W, Paran I, Kesseli R V. Identification of makers linked to diseaseresistance genes by bulked segregation analysis: A rapid method to detect makers in specific genome regions by using segregation population. Proc Natl Acad Sci USA, 1991, 88(21): 98289832.

\[28\]Shen Y, Jiang H, Jin J, et al. Development of genomewide DNA polymorphism database for mapbased cloning of rice genes. Plant Physiol, 2004, 135(3): 11981205.

\[29\]Beemster G T S, Fiorani E, Inze D. Cell cycle: The key to plant growth control. Trends Plant Sci, 2003, 8: 154158.

\[30\]Jia L Q, Zhang B T, Mao C Z, et al. OsCYTINV1 for alkaline/neutral invertase is involved in root cell development and reproductivity in rice (Oryza sativa L. ) . Planta, 2008, 228: 5159.

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