采用恒温培养箱，在黑暗条件下进行发芽试验，观察杂草稻中胚轴伸长的动态过程，分析杂草稻中胚轴伸长过程中籽粒淀粉酶活性和可溶性糖含量的变化及相互关系。结果表明，黑暗条件下发芽后1 d到3 d，杂草稻中胚轴伸长非常慢，发芽后4 d到5 d中胚轴伸长加快，发芽后7天中胚轴伸长逐渐停止，其最终长度显著长于辽粳294。杂草稻发芽过程中，籽粒α淀粉酶活性、β淀粉酶活性和可溶性糖含量均呈逐渐上升趋势。可溶性糖含量与α淀粉酶活性、β淀粉酶活性均呈极显著正相关。中胚轴伸长长度与籽粒α淀粉酶活性、β淀粉酶活性、可溶性糖含量也均呈极显著正相关。杂草稻发芽初期淀粉酶活性增强，促进了籽粒中淀粉水解为可溶性糖，为中胚轴伸长生长提供了物质和能量基础。
马殿荣*,孔德秀,刘晓亮,高齐,丁国华,赵明辉,唐亮,徐正进,陈温福*
. 杂草稻中胚轴伸长动态及其与籽粒淀粉酶活性和可溶性糖含量的关系[J]. 中国水稻科学, 2014
, 28(1)
: 97
-102
.
DOI: 10.3969/j.issn.1001-7216.2014.01.014
The dynamic process of mesocotyl elongation of weedy rice was observed in a germination test in a constant temperature incubator in darkness. The changes in amylase activities in embryos and soluble sugar contents in the process of mesocotyl elongation and their relationship were analyzed. The results showed that during the first three days after germination in darkness, the mesocotyl elongation rate was very slow and accelerated four and five days after germination. The mesocotyl elongation gradually stopped seven days after germination. The mesocotyl of the weedy rice was significantly longer than that of Liaojing 294. In the germination process of weedy rice, soluble sugar contents followed an increasing trend, together with αamylase activity and βamylase activity. Mesocotyl elongation length was significantly correlated with the αamylase activity, βamylase activity and soluble sugar contents. The enhanced amylase activities in the beginning of weedy rice germination promoted starch degradation, supplying material and energy for mesocotyl elengation.
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