2011年，从广西龙胜梯田的水稻根系和稻田土壤中分离到一种孢囊线虫。该线虫形态特征与已报道的旱稻孢囊线虫（Heterodera elachista）相符，其rDNAITS序列与GenBank中旱稻孢囊线虫的rDNAITS序列相似性为97.7%~99.3%。rDNAITS分子发育树表明该线虫与旱稻孢囊线虫以100%的置信度及较小的遗传距离形成一个单系，据此将该线虫鉴定为旱稻孢囊线虫。进一步对本研究获得的10条旱稻孢囊线虫rDNAITS区序列及Tanha Maafi等报道的1条旱稻孢囊线虫rDNAITS序列（AF498391）的酶切位点进行分析，结果发现AluⅠ、AvaⅠ、MvaⅠ和RsaⅠ 4个内切酶的酶切位点在11条序列中完全一致，而Bsh1236Ⅰ、BsuRⅠ和CfoⅠ 3种酶的酶切位点存在变异，其中Bsh1236Ⅰ和BsuRⅠ有2种变异型，CfoⅠ则有3种变异型，表明旱稻孢囊线虫的rDNAITS序列存在DNA异质性。
卓侃1,宋汉达1 ,王宏洪1 ,陶冶1 ,张洪玲1,陆秀红2,黄金玲2,刘志明2 ,廖金铃1,*
. 旱稻孢囊线虫在广西的发生及其rDNAITS异质性分析[J]. 中国水稻科学, 2014
, 28(1)
: 78
-84
.
DOI: 10.3969/j.issn.1001-7216.2014.01.011
In 2011, a cyst nematode was isolated from rice roots and soils in Longsheng Terrace of Guangxi Region, China. Morphological characteristics of the cyst nematode were consistent with previous descriptions of Heterodera elachista. The rDNAITS sequences of the cyst nematode and H. elachista in GenBank shared 97.7%-99.3% identities. The phylogenetic tree based on rDNAITS showed that the cyst nematode was in the same monophyletic clade as H. elachista with 100% support and short branch differences. The results indicated the cyst nematode was Heterodera elachista. The restriction enzyme cutting sites of ten rDNAITS sequences of H. elachista amplified and the one reported by Tanha Maafi (GenBank accession number: AF498391) were analyzed. The restriction enzyme cutting sites of AluⅠ, AvaⅠ, MvaⅠ and RsaⅠ in these 11 sequences were identical, however there were 2 different patterns after Bsh1236Ⅰ and BsuRⅠ digestion, respectively, and 3 different patterns after CfoⅠ digestion. The results show intraspecies heterogeneity in rDNAITS region exists in H. elachista.
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