磷转运蛋白OsPT6为水稻Pht1家族成员之一，具有吸收和转运磷酸盐双重功能。以水稻高产品种武育粳7号的OsPT6超表达转基因材料为试材，研究磷转运蛋白OsPT6在武育粳7号磷素吸收利用中的作用。结果表明：1)OsPT6在武育粳7号的地下部表达丰度较高，同时在地下部和地上部该基因均受缺磷诱导表达量显著增加。2)OsPT6超表达后，转基因植株生长良好。正常供磷和低磷处理35 d后,OsPT6超表达植株地上部和地下部干物质量都显著增加。3)OsPT6超表达转基因材料在不同浓度磷素处理后，各组织全磷含量有所增加，其中营养器官尤为显著。
As one of members of the Pht1 transporters family in rice,the phosphate transporter OsPT6 plays a broad role in Pi uptake, translocation and internal transport throughout the plant. With the OsPT6 overexpressed transgenic rice of Wuyujing 7, a highyielding variety, we studied the roles of OsPT6 on the phosphate uptake and utilization in rice. The main results are the following: 1)OsPT6 is abundantly expressed in roots of Wuyujing 7. Furthermore, the expression levels of OsPT6 in shoots and roots are induced by phosphate deficiency. 2)The overexpression of OsPT6 can promote the growth of plants. The dry weight of shoots and roots of the OsPT6 overexpressed transgenic plants was significantly increased, in both sufficient and deficientPi conditions for 35 days. 3)The total P concentration increases in different tissues and organs of OsPT6 overexpressed transgenic plants, especially in vegetable organs, at different Pisupply levels.
\[1\]Theodorou M E, Plaxton W C. Metabolic adaptations of plant respiration to nutritional phosphate deprivation. Plant Physiol, 1993, 101: 339344.
\[2\]Mimura T. Regulation of phosphate transport and homeostasis in the plant cells. Int Rev Cytol, 1999, 191: 149200.
\[3\]Vance C P, UhdeStone C, Allan D L. Phosphorus acquisition and use: Critical adaptations by plants for securing a nonrenewable resource. New Phytol, 2003, 157: 423447.
\[4\]Schachtman D P, Reid R J, Ayling S M. Phosphorus uptake by plants: From soil to cell. Plant Physiol, 1998, 116: 447453.
\[5\]Raghothama K G. Phosphate acquisition. Annu Rev Plant Physiol Plant Mol Biol, 1999, 150: 665693.
\[6\]Smith F W, Mudge S R, Rae A L, et al. Phosphate transporter in plants. Plant Soil, 2003, 248: 7183.
\[7\]Muchhal U S, Pardo J M, Raghothama K G. Phosphate transporters from the higher plant Arabidopsis thaliana. Proc Natl Acad Sci USA, 1996, 93: 1051910523.
\[8\]Rausch C, Bucher M. Molecular mechanisms of phosphate transport in plants. Planta, 2002, 16: 2337.
\[9\]Mudge S R, Rae A L, Diatloff E, et al. Expression analysis suggests novel roles for members of the Pht1 family of phosphate transporters in Arabidopsis. Plant J, 2002, 31: 341351.
\[10\]Rae A L, Cybinski D H, Jarmey J M, et al. Characterization of two phosphate transporters from barley: Evidence for diverse function and kinetic properties among members of the Pht1 family. Plant Mol Biol, 2003, 53: 2736.
\[11\] Poirier Y, Bucher M. Phosphate transport and homeostasis in Arabidopsis//Somerville C R, Meyerowitz E M. The Arabidopsis Book. Rockville, M D: Am Soc Plant Biol, 2002: 24.
\[12\] Chen A Q, Hu J, Sun S B, et al. Conservation and divergence of both phosphate and mycorrhizaregulated physiological responses and expression patterns of phosphate transporters in solanaceous species. New Phytol, 2007, 173: 817831.
\[13\] Paszkowski U, Kroken S, Roux C, et al. Rice phosphate transporters include an evolutionarily divergent gene specifically activated in arbuscular mycorrhizal symbiosis. Proc Natl Acad Sci USA, 2002, 99: 1332413329.
\[14\] Sun S B, Gu M, Cao Y, et al. A constitutive expressed phosphate transporter, OsPht1;1, modulates phosphate uptake and translocation in phosphatereplete rice. Plant Physiol, 2012, 159: 15711581.
\[15\] Jia H F, Ren H Y, Gu M, et al. Phosphate transporter gene, OsPht1; 8, is involved in phosphate homeostasis in rice. Plant Physiol, 2011, 156: 11641175.
\[16\] Ai P H, Sun S B, Zhao J N, et al. Two rice phosphate transporters, OsPht1;2 and OsPht1;6, have different functions and kinetic properties in uptake and translocation. Plant J， 2009, 57: 798809.
\[17\] 周群演, 蒋红梅. “武运粳7号”主要特性与高产栽培技术. 上海农业科技, 2000(3): 3133.
\[18\] 鲍士旦. 土壤农化分析. 北京： 中国农业出版社， 1999.
\[19\] Elliott G C, Lauchli A. Phosphorus efficiency and phosphate iron interactions in maize. Agron J, 1985, 77: 399403.
\[20\] Siddiqi M Y, Glass A D. Utilization index: A modified approach to the estimation and comparison of nutrient utilization efficiency in plants. J Plant Nutr, 1981, 4: 289302.
\[21\] Shin H, Shin H S, Dewbre G R, et al. Phosphate transport in Arabidopsis: Pht1;1 and Pht1;4 play a major role in phosphate acquisition from both low and highphosphate environments. Plant J, 2004, 39: 629642.
\[22\] Chen A Q, Hu J, Sun S, et al. Conservation and divergence of both phosphate and mycorrhizaregulated physiological responses and expression patterns of phosphate transporters in solanaceous species. New Phytol, 2007, 173: 817831.
\[23\] Misson J, Thibaud M C, Bechtold N, et al. Transcriptional regulation and functional properties of Arabidopsis Pht1;4, a high affinity transporter contributing greatly to phosphate uptake in phosphate deprived plants. Plant Mol Biol, 2004, 55: 727741.
\[24\] Remy E, Cabrito T R, Batista1 R A, et al. The Pht1;9 and Pht1;8 transporters mediate inorganic phosphate acquisition by the Arabidopsis thaliana root during phosphorus starvation. New Phytol, 2012, 195: 356371.
\[25\] Mudge S R, Rae A L, Diatloff E, et al. Expression analysis suggests novel roles for members of the Pht1 family of phosphate transporters in Arabidopsis. Plant J, 2002, 31: 341353.
\[26\] Liu F, Wang Z, Ren H, et al. OsSPX1 suppresses the function of OsPHR2 in the regulation of expression of OsPT2 and phosphate homeostasis in shoots of rice. Plant J, 2010, 62: 508517.
