To reveal protein factors in Laodelphax striatellus Fallen(the small brown planthopper, SBPH) that related to Rice blackstreaked dwarf virus (RBSDV) transmission, the outer capsid protein p10 encoded by RBSDV S10 was selected as bait to screen the SBPH cDNA library. RBSDV p10 gene was inserted to pGBKT7 vector and the bait plasmid pGBKT7p10 was constructed. The result of autoactivation test showed that p10 could not autonomously activate the expression of reporter genes in yeast and had no toxicity to yeast cell. To obtain the interacting proteins with p10, the pGADT7cDNA library plasmids were transformed to the yeast AH109 which contains pGBKT7p10. Three hundred and twentysix positive clones were acquired. The sequencing results show that these positive clones encode 14 proteins, including Actin 1, GAPDH and RACK. These interacting proteins were involved in several processes such as endocytosis, exocytosis and membrane fusion. The interactions between p10 and these proteins may be related to the viral circulation and proliferation in insect vector.
\[1\]方守国, 于嘉林, 冯继东, 等. 我国玉米粗缩病株上发现的水稻黑条矮缩病毒. 农业生物技术学报, 2000, 8(1): 12.
\[2\]Fang S, Yu J, Feng J, et al. Identification of rice blackstreaked dwarf fijivirus in maize with rough dwarf disease in China. Arch Virol， 2001, 146(1): 167170.
\[3\]Zhang H M, Chen J P, Lei J L, et al. Sequence analysis shows that a dwarfing disease on rice, wheat and maize in China is caused by rice blackstreaked dwarf virus. Eur J Plant Pathol， 2001, 107(5): 563567.
\[4\]Bai F W, Yan J, Qu Z C, et al. Phylogenetic analysis reveals that a dwarfing disease on different cereal crops in China is due to rice black streaked dwarf virus (RBSDV). Virus Genes， 2002, 25(2): 201206.
\[5\]Wang H D, Chen J P, Wang A G, et al. Studies on the epidemiology and yield losses from rice blackstreaked dwarf disease in a recent epidemic in Zhejiang province. China. Plant Pathol， 2009, 58(5): 815825.
\[6\]陈声祥, 张巧艳. 我国水稻黑条矮缩病和玉米粗缩病研究进展. 植物保护学报, 2005, 32(1): 97103.
\[7\]Zhang H M, Chen J P, Adams M J. Molecular characterisation of segments 1 to 6 of rice blackstreaked dwarf virus from China provides the complete genome. Arch Virol, 2001, 146(12): 23312339.
\[8\]Wang X F, Zhou G H. Identification of a protein associated with circulative transmission of barley yellow dwarf virus from cereal aphids, Schizaphis graminum and Sitobion avenae. Chin Sci Bull, 2003, 48(19): 2083 2087.
\[9\]Zhang L D, Wang Z H, Wang X B, et al. Two virusencoded RNA silencing suppressors, P14 of Beet necrotic yellow vein virus and S6 of rice black streak dwarf virus. Chin Sci Bull， 2005, 50(4): 305310.
\[10\]Liu H J, Wei C H, Zhong Y W, et al. Rice blackstreaked dwarf virus outer capsid protein P10 has selfinteractions and forms oligomeric complexes in solution. Virus Res， 2007, 127(1): 3442.
\[11\]Wang Q, Tao T, Zhang Y J, et al. Rice blackstreaked dwarf virus P6 selfinteracts to form punctate, viroplasmlike structures in the cytoplasm and recruits viroplasmassociated protein P91. Virol J， 2011, 8(1): 824.
\[12\]Jia M A, Li Y, Lei L, et al. Alteration of gene expression profile in maize infected with a doublestranded RNA fijivirus associated with symptom development. Mol Plant Pathol， 2012, 13(3): 251262.
\[13\]Zhou F, Pu Y Y, Wei T Y, et al. The P2 capsid protein of the nonenveloped rice dwarf phytoreovirus induces membrane fusion in insect host cells. Proc Natl Acad Sci， 2007, 104(49): 1954719552.
\[14\]Yan J, Tomaru M, Takahashi A, et al. P2 protein encoded by genome segment S2 of rice dwarf phytoreovirus is essential for virus infection. Virology， 1996, 224(2): 539541.
\[15\]Tomaru M, Maruyama W, Kikuchi A, et al. The loss of outer capsid protein P2 results in nontransmissibility by the insect vector of rice dwarf phytoreovirus. J Virol， 1997, 71(10): 80198023.
\[16\]Omura T, Yan J, Zhong B X, et al. The P2 protein of rice dwarf phytoreovirus is required for adsorption of the virus to cells of the insect vector. J Virol， 1998, 72(11): 93709373.
\[17\]吴云峰, 杜菊花, 魏宁生. 三种非持久性病毒蚜虫传播专化性研究. 西北农业学报, 1996, 5(1): 3942.
\[18\]Marmonier A, Schellenberger P, Esmenjaud D, et al. The coat protein determines the specificity of virus transmission by Xiphinema diversicaudatum. J Plant Pathol， 2010, 92(1): 275279.
\[19\]Deckert M, Ticchioni M, Bernard A. Endocytosis of GPIanchored proteins in human lymphocytes: Role of glycolipidbased domains, actin cytoskeleton, and protein kinases. J Cell Biol， 1996, 133(4): 791799.
\[20\]Lanier L M, Volkman L E. Actin binding and nucleation by Autographa californica M nucleopolyhedrovirus. Virology，1998, 243(1): 167177.
\[21\]Gottlieb T A, Ivanov I E, Adesnik M, et al. Actin microfilaments play a critical role in endocytosis at the apical but not the basolateral surface of polarized epithelial cells. J Cell Biol, 1993, 120(3): 695710.
\[22\]Harries P A, Schoelz J E, Nelson R S. Intracellular transport of viruses and their components: Utilizing the cytoskeleton and membrane highways. Mol Plant Microbe， 2010, 23(11): 13811393.
\[23\]Yuan Z J, Chen H Y, Chen Q, et al. The early secretory pathway and an actinmyosin Ⅷ motility system are required for plasmodesmatal localization of the NSvc4 protein of Rice stripe virus. Virus Res， 2011, 159(1): 6268.
\[24\]Liedtke C M, Yun C C, Kyle N, et al. PKCdependent regulation of CFTR involves binding to RACK1, a receptor for activated C kinase, and RACK1 binding to NHERF1. J Biol Chem， 2002, 277:2292522933.
\[25\]McCahill A, Warwicker J, Bolger G B, et al. The RACK1 scaffold protein: A dynamic cog in cell response mechanisms. Mol Pharmacol， 2002, 62(6): 12611273.
\[26\]Ron D, MochlyRosen D. An autoregulatory region in protein kinase C: The pseudoanchoring site. Proc Natl Acad Sci， 1995, 92(2): 492496.
\[27\]Ron D, Jiang Z, Yao L N, et al. Coordinated movement of RACK1 with activated βIIPKC. J Biol Chem， 1999, 274(38): 2703927046.
\[28\]Seddas P, Boissinot S, Strub J M, et al. Rack1, GAPDH3, and actin: Proteins of Myzus persicae potentially involved in the transcytosis of beet western yellows virus particles in the aphid. Virology， 2004, 325(2): 399412.
\[29\]Sieczkarski S B, Whittaker G R. Dissecting virus entry via endocytosis. J Gen Virol, 2002, 83(7): 15351545.
\[30\]Glaser P E, Han X, Gross R W. Tubulin is the endogenous inhibitor of the glyceraldehyde 3phosphate dehydrogenase isoform that catalyzes membrane fusion: Implications for the coordinated regulation of glycolysis and membrane fusion. Proc Natl Acad Sci， 2002, 99(22): 1410414109.
\[31\]尚海旭， 井然， 贾弘禔， 等. GAPDH 功能多样性.生理科学进展，2011, 42(5): 371374.
\[32\]Li S, Xiong R Y, Wang X F, et al. Five proteins of Laodelphax striatellus are potentially involved in the interactions between rice stripe virus and vector. PloS One, 2011, 6(10): e26585.
