杂交稻&ldquo;大青棵&rdquo;现象遗传基础剖析

曲丽君1,2,张宏军2,项超2,王辉3,夏加发3,李泽福3,高用明2,*,石英尧1,*

doi:10.3969/j.issn.1001-7216.2013.06.001

中国水稻科学 >

2013 , Vol. 27 >Issue 6: 559 - 568

DOI: https://doi.org/10.3969/j.issn.1001-7216.2013.06.001

研究报告

杂交稻“大青棵”现象遗传基础剖析

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¹安徽农业大学农学院，合肥 230036； ²中国农业科学院作物科学研究所，北京 100081； ³安徽省农业科学院水稻研究所，合肥 230031；

收稿日期: 2013-05-03

修回日期: 2013-07-12

网络出版日期: 2013-11-10

基金资助

盖茨基金资助项目(OPP51587)；国家星火计划资助项目（2011GA710008）；安徽省自然科学基金资助项目（090411016）。

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Study on the Genetic Basis for Abnormal Heading in Hybrid Rice

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¹College of Agronomy, Anhui Agricultural University, Hefei 230036,China; ² Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081,China; ³ Rice Research Institute, Anhui Academy of Agricultural Sciences, Hefei 230031, China;

Received date: 2013-05-03

Revised date: 2013-07-12

Online published: 2013-11-10

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摘要

杂交稻的大青棵现象给我国水稻生产带来了巨大的经济损失，但大青棵产生的遗传基础相关研究尚未见报道。利用蜀恢527/辐恢838群体中的38个导入系为父本，以四个核心不育系II32A、协青早A、冈46A和金23A为母本，构建4个测交群体。在合肥、杭州和广州3个晚稻环境下，对其进行抽穗期和产量相关性状表型评价，结合基因型分析，利用单向（oneway ANOVA）和双向方差分析（twoway ANOVA）检测与感光性相关的QTL及QTL间互作。表型分析表明，55个组合在合肥表现为大青棵，相比之下，这些组合在杭州和广州表现为正常抽穗，但抽穗期延迟。利用单向方差分析，在多个环境或者群体中检测到12个标记与感光性QTL紧密连锁。通过双向方差分析，共检测到31对感光性上位性QTL，其中4对在不同群体和环境下稳定表达。结合基因型分析和上位性分析，发现位点RM331RM2、RM331RM5346、RM3395RM16和RM3325RM53间互作是导致导入系WD57、WD71、WD77、WD78、WD80、WD85和WD88与4个不育系测交组合产生大青棵的主要遗传基础。

关键词： 杂交稻; 大青棵; 抽穗期; 感光性; QTL定位

本文引用格式

曲丽君1,2,张宏军2,项超2,王辉3,夏加发3,李泽福3,高用明2,*,石英尧1,* . 杂交稻“大青棵”现象遗传基础剖析[J]. 中国水稻科学, 2013 , 27(6) : 559 -568 . DOI: 10.3969/j.issn.1001-7216.2013.06.001

Abstract

The problem of abnormal heading in hybrid rice caused great economic losses in recent years. The genetic basis of this phenomenon is still ambiguous and not documented up to now. Four testcrossing populations were constructed using 38 introgression lines (ILs) from Shuhui 527/Fuhui 838 population as male parents and four male sterile lines (II32A, XieqingzaoA, Gang 46A and Jin 23A) as female parents. The phenotypic evaluation was conducted in Hefei, Hangzhou and Guangzhou in late season. QTL associated with photosensitivity (PS) was identified using oneway analysis of variance (ANOVA) and twoway ANOVA methods with the genotypic and phenotypic data. Progeny testing showed that 55 combinations did not head in Hefei, but normally headed with delayed heading date in Hangzhou and Guangzhou. A total of 12 QTL for PS was found in more than two environments or populations. Using twoway ANOVA, 31 epistatic QTLs affecting PS were detected in Hangzhou and Guangzhou, five of which were stably expressed in different populations and environments. The loci interaction between RM331RM2, RM331RM5346, RM3395RM16 and RM3325RM53 played an important role in controlling the formation of Daqingke for combinations from testcrossing WD57, WD71, WD77, WD78, WD80, WD85 and WD88 with fourtypes of male sterile lines.

Key words： hybrid rice; abnormal heading; heading date; photosensitivity; QTL mapping

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