研究稻瘟病菌群体遗传多态性的分子标记方法

穆慧敏1,2,姜华1 ,王艳丽1 ,孙国昌1,*

doi:10.3969/j.issn.1001-7216.2013.05.013

中国水稻科学 >

2013 , Vol. 27 >Issue 5: 545 - 552

DOI: https://doi.org/10.3969/j.issn.1001-7216.2013.05.013

综述与专论

研究稻瘟病菌群体遗传多态性的分子标记方法

展开

¹浙江省农业科学院植物保护与微生物研究所，杭州 310021；²南京农业大学植物保护学院，南京 210095；

收稿日期: 2012-10-22

修回日期: 2012-12-12

网络出版日期: 2013-09-10

基金资助

浙江省自然科学基金资助项目（Y3110537）；国家自然科学基金资助项目（30900933；30970082）；农业部公益性行业项目（201203014）。

收起

Methodology of Genetic Diversity Research on Rice Blast Pathogen Magnaporthe grisea

Expand

¹ Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China;² Institute of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China;

Received date: 2012-10-22

Revised date: 2012-12-12

Online published: 2013-09-10

Fold

摘要

稻瘟病菌的遗传结构经常发生改变，导致田间稻瘟病菌群体具有多态性，严重阻碍了抗病品种在稻瘟病防治上的应用。实时监测和研究稻瘟病菌的遗传变异，了解田间稻瘟病菌群体遗传结构的多样性及其动态对于防治该病具有重大意义。目前，研究稻瘟病菌遗传多样性的常用方法为分子标记技术，主要包括限制性片段长度多态性（RFLP）方法、随机扩增多态性DNA（RAPD）技术、特异性扩增片段（SCAR）标记、简单重复序列（SSR）标记、repPCR技术、扩增片段长度多态性（AFLP）、反转录转座子微卫星扩增多态性（REMAP）。本文就以上这些分子标记方法的实现、优缺点及差异进行了综述，同时也对这些方法在稻瘟病菌遗传多样性方面的研究进行了回顾。

关键词： 稻瘟菌; 遗传结构; 多态性; 分子标记

本文引用格式

穆慧敏1,2,姜华1 ,王艳丽1 ,孙国昌1,* . 研究稻瘟病菌群体遗传多态性的分子标记方法[J]. 中国水稻科学, 2013 , 27(5) : 545 -552 . DOI: 10.3969/j.issn.1001-7216.2013.05.013

Abstract

The genetic structures of the Magnaporthe grisea are changed frequently, resulting in variation of the fungus population in the field which creates lots of challenges on disease control using resistant cultivars. Therefore, understanding the genetic structure variation of rice blast pathogen population in the field and realtime monitoring the dynamic changes are of great significance to control of this disease. Commonly, the methods used on research of the genetic diversity are Restriction Fragment Length Polymorphism (RFLP), Random Amplified Polymorphic DNA (RAPD), Sequenced Characterized Amplified Region (SCAR), Simple Sequence Repeat (SSR), repetitive elementbased Polymerase Chain Reaction (repPCR), Amplified Fragment Length Polymorphism (AFLP), RetrotransposonMicrosatellite Amplified Polymorphism (REMAP). This article reviewed the experimental procedures and the advantages and disadvantages of these molecular methods briefly. The research examples using these different methods were also discussed.

Key words： Magnaporthe grisea; genetic structure; polymorphism; molecular marker

参考文献

\[1\]Couch B C, Kohn L M. A multilocus gene genealogy concordant with host preference indicates segregation of a new species, Magnaporthe oryzae, from Mgrisea.Mycologia, 2002, 94(4): 683693.

\[2\]Ashikawa I, Hayashi N, Yamane H, et al. Two adjacent nucleotidebinding siteleucinerich repeat class genes are required to confer Pikmspecific rice blast resistance. Genetics, 2008, 180(4): 22672276.

\[3\]孙国昌, 杜新法, 陶荣祥, 等. 水稻稻瘟病防治研究进展和21世纪初研究设想. 植物保护, 2000, 26(1): 3436.

\[4\]Hamer J E, Farrall L, Orbach M J, et al. Host speciesspecific conservation of a family of repeated DNA sequences in the genome of a fungal plant pathogen. Proc Natl Acad Sci USA, 1989, 86(24): 99819985.

\[5\]Zhou E, Jia Y, Singh P, et al. Instability of the Magnaporthe oryzae avirulence gene AVRPita alters virulence. Fungal Genet Biol, 2007, 44(10): 10241034.

\[6\]George M L, Nelson R J, Zeigler R S, et al. Rapid population analysis of Magnaporthe grisea by using repPCR and endogenous repetitive DNA sequences. Phytopathology, 1998, 88(3): 223229.

\[7\]Tredway L P, Stevenson K L, Burpee L L. Genetic structure of Magnaporthe grisea populations associated with St. Augustinegrass and tall fescue in Georgia. Phytopathology, 2005, 95(5): 463471.

\[8\]Farman M L, Kim Y S. Telomere hypervariability in Magnaporthe oryzae.Mol Plant Pathol, 2005, 6(3): 287298.

\[9\]Sharma T, Chauhan R, Singh B, et al. RAPD and pathotype analyses of Magnaporthe grisea populations from the north‐western Himalayan region of India. Phytopathol, 2002, 150(11/12): 649656.

\[10\]JavanNikkhah M, McDonald B A, Banke S, et al. Genetic structure of Iranian Pyricularia grisea populations based on repPCR fingerprinting. Eur J Plant Pathol, 2004, 110(9): 909919.

\[11\]Karaoglu H, Lee C M, Meyer W. Survey of simple sequence repeats in completed fungal genomes. Mol Biol Evol, 2005, 22(3): 639649.

\[12\]Labbé J, Murat C, Morin E, et al. Survey and analysis of simple sequence repeats in the Laccaria bicolor genome, with development of microsatellite markers. Curr Gene, 2010, 57(2): 7588.

\[13\]Kachroo P, Leong S A, Chattoo B B. Pot2, an inverted repeat transposon from the rice blast fungus Magnaporthe grisea.Mol Gen Genet, 1994, 245(3): 339348.

\[14\]Farman M L, Taura S, Leong S A. The Magnaporthe grisea DNA fingerprinting probe MGR586 contains the 3′ end of an inverted repeat transposon. Mol Gen Genet, 1996, 251(6): 675681.

\[15\]Levy M, Romao J, Marchetti M A, et al. DNA fingerprinting with a dispersed repeated sequence resolves pathotype diversity in the rice blast fungus. Plant Cell, 1991, 3(1): 95102.

\[16\]Romao J, Hamer J E. Genetic organization of a repeated DNA sequence family in the rice blast fungus. Proc Natl Acad Sci USA, 1992, 89(12): 53165320.

\[17\]Ren L, Wang Q, Bai R. Pot2 fingerprint of Magnaporthe grisea DNA from some rice varieties in Jilin Province. J Jilin Agric Univ, 2005, 27(4): 369372.

\[18\]Farman M L, Tosa Y, Nitta N, et al. MAGGY, a retrotransposon in the genome of the rice blast fungus Magnaporthe grisea.Mol Gen Genet, 1996, 251(6): 665674.

\[19\]Moody S F, Tyler B M. Use of nuclear DNA restriction fragment length polymorphisms to analyze the diversity of the Aspergillus flavus group: A. flavus, A. parasiticus, and A. nomius.Appl Environ Microbiol, 1990, 56(8): 24532461.

\[20\]Levy M, Correavictoria F J, Zeigler R S, et al. Genetic diversity of the rice blast fungus in a disease nursery in Colombia. Phytopathology, 1993, 83(12): 14271433.

\[21\]Zeigler R, Thome J, Nelson R, et al. Lineage exclusion: A proposal for linking blast population analysis to rice breeding//The Rice Blast Disease, Baltimore, Maryland, USA： John Hopkins Press, 1994: 267291.

\[22\]Chadha S, Gopalakrishna T. Retrotransposonmicrosatellite amplified polymorphism (REMAP) markers for genetic diversity assessment of the rice blast pathogen (Magnaporthe grisea). Genome, 2005, 48(5): 943945.

\[23\]Williams J G K, Kubelik A R, Livak K J, et al. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res, 1990, 18(22): 65316535.

\[24\]Bernardo M A, Naqvi N, Leung H, et al. A rapid method for DNA fingerprinting of the rice blast fungus Pyricularia grisea.Inter Rice Res Notes, 1993, 18(1): 4850.

\[25\]Busso C, Nobuyoshi K E, Franco F A, et al. Genetic and molecular characterization of pathogenic isolates of Pyricularia grisea from wheat (Triticum aestivum Lam.) and triticale (x Triticosecale Wittmack) in the state of Parana, Brazil. Rev Iberoam Micol, 2007, 24(2): 167170.

\[26\]Paran I, Michelmore R. Development of reliable PCRbased markers linked to downy mildew resistance genes in lettuce. Theor Appli Genet, 1993, 85(8): 985993.

\[27\]Brondani C, Brondani R P V, Garrido L R, et al. Development of microsatellite markers for the genetic analysis of Magnaporthe grisea.Genet Mol Biol, 2000, 23(4): 753762.

\[28\]Zheng Y, Zhang G, Lin F, et al. Development of microsatellite markers and construction of genetic map in rice blast pathogen Magnaporthe grisea.Fungal Genet Biol, 2008, 45(10): 13401347.

\[29\]王艳丽, Kaye C, Bordat A, 等. 稻瘟病菌株CH63和TH16杂交组合的遗传图谱构建及无毒基因定位. 中国水稻科学, 2005, 19(2): 160166.

\[30\]Kim N S, Park N I, Kim S H, et al. Isolation of TC/AG repeat microsatellite sequences for fingerprinting rice blast fungus and their possible horizontal transfer to plant species. Mol and Cells, 2000, 10(2): 127134.

\[31\]Suzuki F, Arai M, Yamaguchi J. DNA fingerprinting of Pyricularia grisea by repPCR using a single primer based on the terminal inverted repeat from either of the transposable elements Pot2 and MGR586. J Gene Plant Pathol, 2006, 72(5): 314317.

\[32\]Prabhu A S, Filippi M C, Araujo L G, et al. Genetic and phenotypic characterization of isolates of Pyricularia grisea from the rice cultivars Epagri 108 and 109 in the state of Tocantins. Fitopatol Bras, 2002, 27(6): 566573.

\[33\]Chen Q H, Wang Y C, Zheng X B. Genetic diversity of Magnaporthe grisea in China as revealed by DNA fingerprint haplotypes and pathotypes. J Phytopathol, 2006, 154(6): 361369.

\[34\]Prabhu A S. Virulence and repPCR analysis of Pyricularia grisea isolates from two Brazilian upland rice cultivars. Fitopatol Bras, 2007, 32(1): 1320.

\[35\]Kumar J, Nelson R J, Zeigler R S. Population structure and dynamics of Magnaporthe grisea in the Indian Himalayas. Genetics, 1999, 152(3): 971984.

\[36\]Park S Y, Milgroom M G, Han S S, et al. Diversity of pathotypes and DNA fingerprint haplotypes in populations of Magnaporthe grisea in Korea over two decades. Phytopathology, 2003, 93(11): 13781385.

\[37\]Takan J P, Chipili J, Muthumeenakshi S, et al. Magnaporthe oryzae populations adapted to finger millet and rice exhibit distinctive patterns of genetic diversity, sexuality and host interaction. Mol Biotechnol, 2011, 50(2): 145158.

\[38\]Sirithunya P, Sreewongchai T, Sriprakhon S, et al. Assessment of genetic diversity in Thai isolates of Pyricularia grisea by Random Amplification of Polymorphic DNA. J Phytopathol, 2008, 156(4): 196204.

\[39\]Tharreau D, Soubabere O, Lebrun M H, et al. Developing and using SCAR (Sequence Characterized Amplified Regions) to analyze Magnaporthe grisea populations pathogenic to rice. Advances in Rice Genetics. Los Banos, Laguna, Philippines,   2003: 619621.

\[40\]Douhan G W, de la Cerda K A, Huryn K L, et al. Contrasting genetic structure between Magnaporthe grisea populations associated with the golf course turfgrasses Lolium perenne (perennial ryegrass) and Pennisetum clandestinum (kikuyugrass). Phytopathology, 2011, 101(1): 8591.

\[41\]Flor H H. Current status of the geneforgene concept. Annu Rev Phytopathol, 1971, 9: 275296.

\[42\]Hammond K E, Jones J D G. Plant disease resistance genes. Annu Rev Plant Physiol Plant Mol   Biol, 1997, 48: 575607.

\[43\]Lauge R, De Wit P J. Fungal avirulence genes: Structure and possible functions. Fungal Genet Biol, 1998, 24(3): 285297.

\[44\]Zheng Y, Zheng W, Lin F, et al. AVR1CO39 is a predominant locus governing the broad avirulence of Magnaporthe oryzae 2539 on cultivated rice (Oryza sativa L.). Mol Plant Microbe Inter, 2011, 24(1): 1317.

\[45\]孙国昌, 孙漱沅, 申宗坦, 等. 中国部分抗稻瘟病水稻品种的抗病特性和菌株致病性研究. 浙江农业学报, 1992(1): 17.

\[46\]柴荣耀, 杜新法, 毛雪琴, 等. 水稻不同类型品种上的稻瘟病菌群体小种的演变. 浙江农业学报, 1999(6): 297300.

\[47\]Liu J, Wang X, Mitchell T, et al. Recent progress and understanding of the molecular mechanisms of the riceMagnaporthe oryzae interaction. Mol Plant Pathol, 2010, 11(3): 419427.

\[48\] Kerstens H H D,   Crooijmans P M A, Veenendaal A, et al. Large scale single nucleotide polymorphism discovery in unsequenced genomes using second generation high throughput sequencing technology: Applied to turkey. BMC Genom, 2009, 10: 479.

\[49\]Xue M F, Yang J, Li Z G, et al. Comparative analysis of the genomes of two field isolates of the rice blast fungus Magnaporthe oryza.PLoS Genet, 2012, 8(8): 112.

Options

文章导航

模态框（Modal）标题

摘要

本文引用格式

Abstract

参考文献