实验技术

利用半人工饲料大规模简便化饲养二化螟

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  • 1中国水稻研究所 水稻生物学国家重点实验室, 杭州 310006;2浙江省植物保护检疫局,  杭州 310020;

收稿日期: 2013-01-04

  修回日期: 2013-03-27

  网络出版日期: 2013-09-10

基金资助

国家转基因生物重大科技专项(2011ZX08012004); 国家自然科学基金资助项目(31171840); 浙江省自然科学基金资助项目(Y3100699)。

A Technique for Rearing Chilo suppressalis in the Large Scale with an Oligidic Diet in Laboratory

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  • 1 China National Rice Research Institute, Hangzhou 310006, China;  2 Zhejiang Provincial Plant Protection and Quarantine Bureau, Hangzhou 310020, China;

Received date: 2013-01-04

  Revised date: 2013-03-27

  Online published: 2013-09-10

摘要

利用一个新的二化螟幼虫半人工饲料配方,通过改进幼虫饲养盒和采用专用化蛹装置,成功地实现了二化螟的人工大规模简便化饲养,极大地提高了二化螟人工饲料饲养效率。与其他方法相比,该法饲养得到的第3代二化螟幼期(幼虫+蛹)历期(38.6 d)相对较短,雌雄蛹质量(68.9 mg、53.4 mg)较大。说明本方法采用的幼虫饲料配方可以较好地满足幼虫的营养需求。用该饲料鉰养化蛹率可达40%,即一个幼虫饲养盒可以收获80粒蛹。初步估计得到2400粒二化螟蛹,仅需要投入30个工时,该方法可以有效满足大规模继代化饲养的要求。

本文引用格式

胡阳1,郑永利2,曹国连1,傅强1,* . 利用半人工饲料大规模简便化饲养二化螟[J]. 中国水稻科学, 2013 , 27(5) : 535 -538 . DOI: 10.3969/j.issn.1001-7216.2013.05.011

Abstract

A new rearing system was  adopted  to  efficiently rear Chilo suppressalis in  the large scale. A new artificial diet was provided and two new equipments (the larvae growing box and the pupation ring) were also used for this system. The developmental period from neonate to pupation, the weights of female and male pupae of the 3rd generation in laboratory were compared to other reports. Results showed that the developmental period from neonate to pupation reared by our system is better than other artificial diets. Both female and male pupae reared by our system is also heavier than most of other artificial diets, but little lighter than some fresh materials such as wateroats, sweat corn. The pupation rate from the matured eggs to the pupal stage is about 40%, which means we can collect 80 pupae from each larval box. Based on our primary estimation, it takes 30 h/person to harvest 2400 pupae. In overall, we think this system is labor saving and economic for rearing C. suppressalis in a large scale in laboratory.

参考文献

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