根据Wxmq基因存在的单核苷酸变异，利用四引物扩增受阻突变体系PCR（Tetraprimer ARMSPCR）的方法设计特异引物，对12个水稻品种（或品系）以及武育粳3号（不含Wxmq基因）/关东194（含Wxmq基因）的F2分离群体进行扩增，依据其PCR产物的带型，可以准确区分出Wxmq基因纯合、非Wxmq基因纯合以及杂合基因型三种类型，其电泳结果与成熟后对胚乳外观特性鉴定完全一致。因此，作为一种简单、低成本的实用技术，四引物ARMSPCR可以广泛用于Wxmq基因水稻的资源鉴定和分子标记辅助育种。
陈涛,骆名瑞,张亚东,朱镇,赵凌,赵庆勇,周丽慧,姚姝,于新,王才林*
. 利用四引物扩增受阻突变体系PCR技术检测水稻低直链淀粉含量基因Wxmq[J]. 中国水稻科学, 2013
, 27(5)
: 529
-534
.
DOI: 10.3969/j.issn.1001-7216.2013.05.010
To improve the efficiency of selection for Wxmq gene in breeding, we designed specific primers by tetraprimer Amplification Refractory Mutation System PCR (ARMSPCR) method, according to the single nucleotide mutation in Wxmq gene to analyze 12 rice varieties (or lines) and the F2 segregation population derived from the cross of lowamylose content variety Kanto 194 (with Wxmq gene) and normal variety Wuyujing 3(without Wxmq gene). The results of PCR detection indicated that three genotypes including the homozygous genotype with Wxmq, the homozygous genotype without Wxmq and the heterozygous genotype could be clearly distinguished according to band types, and it was completely consistent with the result of phenotypic analysis. Therefore, as a simple and lowcost technique，the tetraprimer ARMSPCR could be widely used to identify rice resources with Wxmq gene and applied in molecular markerassisted breeding.
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