水稻幼苗叶色温敏感突变体的鉴定及其基因定位

江少华1 ,周华1 ,林冬枝1, * ,董彦君1, * ,叶胜海2 ,张小明2

doi:10.3969/j.issn.1001-7216.2013.04.004

中国水稻科学 >

2013 , Vol. 27 >Issue 4: 359 - 364

DOI: https://doi.org/10.3969/j.issn.1001-7216.2013.04.004

研究报告

水稻幼苗叶色温敏感突变体的鉴定及其基因定位

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¹上海师范大学生命与环境科学学院植物遗传与功能基因研究室, 上海 200234； ²浙江省农业科学院，杭州 310021；

收稿日期: 2012-09-25

修回日期: 2013-01-16

网络出版日期: 2013-07-10

基金资助

国家自然科学基金资助项目(30971552)；上海市科委项目(09DJ1400505, 0939191200,10DZ2271800, 12ZR1422000)；上海市教委项目(J50401)。

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Identification and Gene Mapping of a Thermosensitive Leafcolor Mutant at Seedling Stage in Rice

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¹ Laboratory of Plant Genetics and Functional Genes, College of Life and Environment Sciences, Shanghai Normal University, Shanghai 200234, China; ²Zhejiang Academy of Agricultural Sciences, Hangzhou 310021， China;

Received date: 2012-09-25

Revised date: 2013-01-16

Online published: 2013-07-10

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摘要

在粳稻嘉花1号水稻品种经60Co γ辐照后代中筛选到一个幼苗叶色温敏感突变体tsl11。与野生型嘉花1号相比，该突变体在20℃下，幼苗叶色呈黄色；叶绿素a、叶绿素b和类胡萝卜素含量分别下降94.6%、88.2%和91.5%；且电镜结果显示叶绿体内部结构不完整。而在高温（32℃）条件下突变体的叶色表型、光合色素含量和叶绿体发育与野生型基本一致，显示出明显的温度敏感性。遗传分析表明，该突变体叶色性状受1对隐性核基因控制，暂命名为tsl11（thermosensitive leafcolor11)。以该突变体与籼稻培矮64S杂交，构建4928株突变型F2，利用SSR和InDel分子标记将tsl11基因定位于水稻第11染色体上的ID14506与ID14653之间的147 kb的区间内。

关键词： 温敏感; 叶色突变体; 叶绿素含量; 基因定位

本文引用格式

江少华1 ,周华1 ,林冬枝1, * ,董彦君1, * ,叶胜海2 ,张小明2 . 水稻幼苗叶色温敏感突变体的鉴定及其基因定位[J]. 中国水稻科学, 2013 , 27(4) : 359 -364 . DOI: 10.3969/j.issn.1001-7216.2013.04.004

Abstract

A thermosensitive leafcolor mutant, tsl11, was selected from the progeny of the japonica rice variety Jiahua 1 by 60Co γradiation. Compared with the wildtype parent Jiahua 1 at 20℃, the mutant was characterized yellowseedling，decreased contents of chlorophyll a,chlorophyll b and carotenoid by 94.6%, 88.2% and 91.5%,respectively.Simultaneously, the result of electron microscopic observation revealed that the structure of chloroplasts in the tsl11 was imperfect seriously. However,the mutant was similar to wild type at high temperatures(32℃).These results indicated that the mutant was obviously thermosensitive. Genetic analysis showed that the mutant trait was controlled by a single nuclear recessive gene, tentatively designated as tsl11. Moreover, the mapping population of 4928 F2 mutant individuals derived from Pei’ai 64S/tsl11 were constructed, then using SSR and InDel markers, the tsl11 gene was finally mapped to a region of 147 kb between ID14506 and ID14653 on chromosome 11.

Key words： thermosensitive; leafcolor mutant; chlorophyll content; gene mapping

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