A genetic linkage map consisting 207 DNA markers, was constructed based on a recombinant inbred line(RIL) population derived from a cross between indica Luhui 99 and japonica Nipponbare. The markers in the linkage map distributed on all the 12 rice chromosomes and covered 2397 cM of the genome with the average distance between the markers being 12.29 cM. In 2011, the parents and 188 RILs were grown in the experimental field at Luzhou and Deyang experimental farm of the Institute of Rice and Sorghum, Sichuan Academy of Agricultural Sciences. The statistic software of QTL Network 2.0 was applied to detect QTL, QTL×QTL epstatic effects and QTL×environment(QE)interaction for seven traits, including number of panicles per plant, number of spikelet per panicle, number of filled grains per panicle, seed setting rate, 1000grain weight, grain yield per plant, plant height. A total of 22 QTLs with significant additive effects covering all chromosomes except chromosomes 6, 11 and 12 and two QTL with significant QE interactions were detected. Seven pairs of QTLs showing significant additive ×additive epistatic effects were detected except three traits including number of spikelet per panicle, number of filled grains per panicle, and seed setting rate. Genetic contributions were generally low for QTL showing epistatic effects. No significant interaction between epistatic QTL and environment was detected.
\[1\]张启发. 绿色超级稻的构想与实践. 北京: 科学出版社，2009: 1.
\[2\]杨守仁, 张步龙, 陈温福, 等. 水稻超高产育种的理论和方法. 沈阳农业大学学报, 2003, 34(5): 321323.
\[3\]黄耀祥, 林青山. 水稻超高产、特优质株型模式的构想和育种实践. 广东农业科学, 1994(4): 16.
\[4\]袁隆平. 杂交水稻超高产育种. 杂交水稻,1997(6): 49.
\[5\]程式华, 翟虎渠. 水稻亚种间超高产杂交组合若干株型因子的比较. 作物学报, 2000, 26(6): 713718.
\[6\]周开达, 刘太清, 马玉清, 等. 杂交水稻亚种间重穗型组合选育: 杂交水稻超高产育种的理论与实践. 四川农业大学学报, 1995, 13(4): 403407.
\[7\]Khush G S. Prospects and approaches to increasing the genetic yield potential of rice// Evenson R E. Rice Research in Asia, Progress and Priorities. CAB International and IRRI, 1996: 5971.
\[8\]朱旭东, 张国平, 姚海根, 等. 浙江早稻新品系的产量及其农艺性状比较. 浙江农业科学, 2007(1): 6973.
\[9\] Paterson A H, Lander E S, Hewitt J D, et al. Resolution of quantitative traits into Mendelian factors by using a complete linkage map of restriction fragment length polymorphisms. Nature, 1988, 335(6192):721726.
\[10\]Lu F, Ammiraju J S, Sanyal A, et al. Comparative sequence analysis of MONOCULM1orthologous regions in 14 Oryza genomes. Proc Natl Acad Sci, 2009, 106(6): 20712076.
\[11\]Yan H F, Saika H, Maekawa M, et al. Rice tillering dwarf mutant dwarf3 has increased leaf longevity during darknessinduced senescence or hydrogen peroxideinduced cell death. Genes Genetic Systems, 2007, 82(4): 361366.
\[12\]Li Y, Fan C, Xing Y, et al. Natural variation in GS5 plays an important role in regulating grain size and yield in rice. Nat Genet, 2011, 43(12): 12661269.
\[13\]Song X J, Huang W, Shi M, et al. A QTL for rice grain width and weight encodes a previously unknown RINGtype E3 ubiquitin ligase. Nat Genet, 2007, 39(5): 623630.
\[14\]Fan C, Xing Y Z, Mao H L, et al. GS3 participates in stigma exsertion as well as seed length in rice. Breeding Sci, 2011, 61(3): 244250.
\[15\]Taguchishiobara F, Kawagoe Y, Kato H, et al. A lossoffunction mutation of rice DENSE PANICLE 1 causes semidwarfness and slightly increased number of spikelets. Breeding Sci, 2011, 61(1): 1725.
\[16\]Ashikari M, Sakakibara H, Lin S, et al. Cytokinin oxidase regulates rice grain production. Science, 2005, 309(5735): 741745.
\[17\]Li M, Tang D, Wang K, et al. Mutations in the Fbox gene LARGER PANICLE improve the panicle architecture and enhance the grain yield in rice. Plant Biotechnol J, 2011, 9(9): 10021013.
\[18\]Li S, Qian Q, Fu Z, et al. Short panicle1 encodes a putative PTR family transporter and determines rice panicle size. Plant J, 2009, 58(4): 592605.
\[19\]Wang H D, Makeen K, Yan Y, et al. OsSIZ1 regulates the vegetative growth and reproductive development in rice. Plant Mol Biol Rep, 2011, 29(2): 411417.
\[20\]Ni J, Wang G H, Zhu Z X, et al. OsIAA23mediated auxin signaling defines postembryonic maintenance of QC in rice. Plant J, 2011, 68(3): 433442.
\[21\]Xue W, Xing Y, Weng X, et al. Natural variation in Ghd7 is an important regulator of heading date and yield potential in rice. Nat Genet, 2008, 40(6): 761767.
\[22\]Yi X H, Zhang Z J, Zeng S Y, et al. Introgression of qPE91 allele, conferring the panicle erectness, leads to the decrease of grain yield per plant in japonica rice (Oryza sativa L.). J Gen Genom, 2011, 38(5): 217223.
\[23\]刘坚, 陶红剑, 施思, 等. 水稻穗型的遗传和育种改良. 中国水稻科学, 2012, 26(2): 227234.
\[24\]McCouch S R. Gene nomenclature system for rice. Rice, 2008, 1(1): 7284.
\[25\]张涛. 水稻糙米蛋白质含量的QTL定位及香稻的资源研究. 雅安: 四川农业大学, 2007.
\[26\]Wang D L, Zhu J, Li Z L, et al. Mapping QTLs with epistatic effects and QTL × environment interactions by mixed linear model approaches. Theor Appl Genet, 1999, 99(7): 12551264.
\[27\]Yang J, Zhu J. Predicting superior genotypes in multiple environments based on QTL effects. Theor Appl Genet, 2005, 110(7): 12681274.
\[28\]Holland J B. Genetic architecture of complex of traits in plants. Curr Opin Plant Biol, 2007, 10:156161.
\[29\]Xiao J, Li J, Yuan L, et al. Identification of QTLs affecting traits of agronomic importance in a recombination inbred population derived from a subspecific rice cross. Theor Appl Genet, 1996, 92(2): 230244.
\[30\]李平. 水稻分子图谱的构建与基因分析\[学位论文\]. 雅安: 四川农业大学, 1994.
\[31\]Huang X, Qian Q, Liu Z, et al. Natural variation at the DEP1 locus enhances grain yield in rice. Nat Genet, 2009, 41(4): 494497.
\[32\]Liu G F, Jian Y, Xu H M, et al. Influence of epistasis and QTL × environment interaction on heading date of rice (Oryza sativa L.). J Gen Genom, 2007, 34(7): 608615.
\[33\]Gao Y M, Zhu J. Mapping QTLs with digenic epistasis under multiple environments and predicting heterosis based on QTL effects. Theor Appl Genet, 2007, 115(3): 325333.
\[34\]曹立勇, 占小登, 庄杰云, 等. 水稻产量性状的QTL定位与上位性分析. 中国农业科学, 2003, 36(11): 12411247.
\[35\]Lu C, Shen L, Tan Z, et al. Comparative mapping of QTLs for agronomic traits of rice across environments using a doubled haploid population. Theor Appl Genet, 1996, 93(8):12111217.
\[36\]Xing Y Z, Tan Y F, Hua J P, et al. Characterization of the main effects, epistatic effects and their environmental interactions of QTLs on the genetic basis of yield traits in rice. Theor Appl Genet, 2002, 105(2): 248257.
