转小萝卜&gamma;硫堇蛋白基因RsAFP1水稻的获得及其稻瘟病抗性初步鉴定

李海青1,2,# ,李臻1,# ,王莹莹1 ,柳絮1 ,王庆国1 ,姚方印1 ,刘炜 1,2,*

doi:10.3969/j.issn.1001-7216.2013.04.001

中国水稻科学 >

2013 , Vol. 27 >Issue 4: 335 - 343

DOI: https://doi.org/10.3969/j.issn.1001-7216.2013.04.001

研究报告

转小萝卜γ硫堇蛋白基因RsAFP1水稻的获得及其稻瘟病抗性初步鉴定

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¹山东省农业科学院高新技术研究中心/山东省作物遗传改良与生态生理重点实验室, 济南 250100；²山东师范大学生命科学学院, 济南 250014;

收稿日期: 2012-10-31

修回日期: 2012-12-03

网络出版日期: 2013-07-10

基金资助

国家转基因植物新品种培育重大专项（2009ZX08001010B）。

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Transformation of γthionin Gene RsAFP1 into Rice and Preliminary Idenfication of Its Blast Resistance

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¹ HighTech Research Center, Shandong Academy of Agricultural Sciences/Key Laboratory of Genetic Improvement, Ecology and Physiology of Crops, Jinan 250100, China; ² College of Life Sciences, Shandong Normal University, Jinan 250014, China；

Received date: 2012-10-31

Revised date: 2012-12-03

Online published: 2013-07-10

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摘要

将从小萝卜中分离的硫堇蛋白类基因RsAFP1通过基因工程的方法导入带有泛素Ubiquitin启动子及抗除草剂Bar基因的植物双元表达载体pCAMBIA1300中，经农杆菌介导对圣稻13进行遗传转化。对获得的阳性转基因植株进行稻瘟病菌体外及室内苗期稻瘟病菌接种抗性鉴定，结合田间自然发病情况，对转基因植株的抗稻瘟病性进行综合评价。初步结果表明，外源RsAFP1基因的导入可在一定程度上提高水稻对稻瘟病的抗性。

关键词： 稻瘟病； γ 硫堇蛋白； RsAFP1基因；抗病性

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李海青1,2,# ,李臻1,# ,王莹莹1 ,柳絮1 ,王庆国1 ,姚方印1 ,刘炜 1,2,* . 转小萝卜γ硫堇蛋白基因RsAFP1水稻的获得及其稻瘟病抗性初步鉴定[J]. 中国水稻科学, 2013 , 27(4) : 335 -343 . DOI: 10.3969/j.issn.1001-7216.2013.04.001

Abstract

A γthionin protein gene termed RsAFP1 isolated from the radish by PCR was introduced into the modified binary vector pCAMBIA1300. Six positive transformants were obtained by agrobacteriummediated transformation to rice callus. Further, the in vitro bacterial inhibition test and indoor rice blast fungus inoculation to rice seedling were conducted.In combination with the natural incidence in field， the resistance of transgenic lines were comprehensive evaluated. The results showed that introduction of RsAFP1 gene could improve rice blast resistance.

Key words： rice blast; γthionin; RsAFP1 gene; disease resistance

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