用2个耐盐水稻品种和2个盐敏感型水稻品种为材料，研究盐胁迫对水稻植株生物量积累、光合特性等生理特性的影响。结果表明，在盐胁迫条件下，耐盐水稻品种和盐敏感型水稻品种地上和根系的干鲜质量均呈下降趋势，其中，以盐敏感型水稻品种的地上部鲜质量与根系干质量的下降最为显著。盐胁迫条件下，水稻叶片的净光合速率（Pn）、气孔导度（Gs）、蒸腾作用（Tr）和表观叶肉导度（AMC）均呈不同程度的下降趋势。其中，耐盐水稻品种Pn、Gs、Tr和Pn/Ci的下降均低于盐敏感型水稻品种。同时，耐盐品种水分利用效率（WUE）也高于盐敏感型品种。盐胁迫条件下，耐盐水稻品种和盐敏感型水稻品种的胞间二氧化碳浓度（Ci）变化并不明显，气孔限制百分率（Ls）均较低，品种间差异也不显著，而表观叶肉导度显著下降，由此推测盐胁迫条件下Pn的下降并非因为气孔的限制，而与RuBPCase活性的下降有关。盐胁迫条件下水稻叶片和根系的渗透性调节物质脯氨酸、总氨基酸和可溶性糖含量上升，其中以可溶性糖含量上升最为显著（P＜0.01）。盐胁迫下叶片保护酶SOD、POD和CAT活性增强，膜透性增强，丙二醛含量增加，同时，根系活力下降。
With two salttolerant rice varieties and two saltsensitive varieties as material, the influence of salt stress on plant biomass accumulation, photosynthetic characteristics and the other physiological characteristics was studied. The results showed that the dry weight and fresh weight of shoot and root of salttolerant rice varieties and saltsensitive varieties were declined under salt stress. The fresh weight of shoot and dry weight of roots were declined significantly. The net photosynthetic rate, stomatal conductance, transpiration and apparent mesophyll conductance were declined to different degrees under salt stress. The drop of Pn, Gs, Tr and Pn/Ci of salttolerant varieties was less than saltsensitive varieties. The water use efficiency(WUE) of salttolerant varieties was higher than that of saltsensitive ones at the same time. The intercellular CO2 concentration (Ci) of salttolerant varieties and saltsensitive ones was not changed obvirously under salt stress. The stomatal limitation percentage (Ls) under salt stress was low and the difference among the varieties was not significant. And apparent mesophyll conductance (Pn/Ci) was changed significantly. The decline of Pnresulted from the decline of RuBPCase rather than from stomatal limitation. The permeability regulation substance contents of leaf and root such as soluble sugar and proline, total amino acids were increasd under salt stress with significant increase in soluble sugar contents (P < 0.01). The protective enzyme SOD, POD and CAT activities were enhanced, as well as the membrane permeability and the MDA content, the root vigor was declined under salt stress.
\[1\]Koyro H W，Eisa S S. Effect of salinity on composition, viability and germination of seeds of Chenopodium quinoa Willd. Plant Soil, 2008，302：7990.
\[2\]Herralde F D，Biel C，Savé R，et al. Effect of water and salt stresses on the growth, gas exchange and water relations in Argyranthemum coronopifolium plants. Plant Sci, 1998，139：917.
\[3\]Koca H，Bor M，zdemir F，et al. The effect of salt stress on lipid peroxidation, antioxidative enzymes and proline content of sesame cultivars. Environ Exper Bot，2007，60：344351.
\[4\]Soussi M，Ocaa A，Lluch C. Effects of salt stress on growth, photosynthesis and nitrogen fixation in chickpea(Cicer arietinum L.). Exp Bot，1998，49：13291337.
\[5\]谷艳芳，丁圣彦，李婷婷，等. 盐胁迫对冬小麦幼苗干物质分配和生理生态特性的影响. 生态学报，2009，29（2）：840845.
\[6\]Mahmood I A，Navaz S，Aslam M. Scerrning of rice (Oryza sativa L.) genotypes against NaCl salinity. Int J Agric Biol，2000，1（2）：147150.
\[7\]Silva E C，Nogueira R J M C，Araújo F P，et al. Physiological responses to salt stress in young umbu plants. Environ Exp Bot，2008，63：147157.
\[8\]Summart J，Thanonkeo P，Panichajakul S，et al. Effect of salt stress on growth, inorganic ion and praline accumulation in Thai aromatic rice, Khao Dawk Mali 105, callus culture. Afric J Biotechnol，2010，9（2）：145152.
\[9\]宁建凤，郑青松，杨少海，等. 高盐胁迫对罗布麻生长及离子平衡的影响. 应用生态学报，2010，21（2）：325330.
\[10\]ChaUm S，Nhung N T H，Kirdmanee C. Effect of mannitoland saltinduced isoosmotic stress on proline accumulation, photosynthetic abilities and growth characters of rice cultivars(Oryza sativa L.spp. indica ). Pakis J Bot，2010，42（2）：927941.
\[11\]James R A，Munns R，Caemmerer S V，et al. Photosynthetic capacity is related to the cellular and subcellular partitioning of Na+, K+ and Cl- in saltaffected barley and durum wheat. Plant, Cell Environ，2006，29：21852197.
\[12\]王仁雷，华春，刘友良. 盐胁迫对水稻光合特性的影响. 南京农业大学学报，2002，25(4)：1114.
\[13\]Raza S H， Athar H R， Ashraf M， et al. Glycinebetaineinduced modulation of antioxidant enzymes activities and ion accumulation in two wheat cultivars differing in salt tolerance. Environ Exp Bot，2007，60：368376.
\[14\] Munns R，Tester M. Mechanisms of salinity tolerance. Plant Biol, 2008，59：651681.
\[15\]王宁，曹敏健，于佳林. NaCl胁迫对玉米幼苗有机渗透调节物质的影响. 玉米科学，2009，17（4）：6165，69.
\[16\]梁正伟，杨福，王志春，等. 盐碱胁迫对水稻主要生育性状的影响. 生态环境，2004，13（1）：4346.
\[17\]张瑞珍，邵玺文，童淑媛，等. 盐碱胁迫对水稻源库与产量的影响. 中国水稻科学，2006，20（1）：116118.
\[18\] 张治安，陈展宇. 植物生理学实验技术. 长春：吉林大学出版社，2008.
\[19\]张殿忠，汪沛洪，赵会贤. 测定小麦叶片游离脯氨酸的方法. 植物生理学通讯，1990（4）：6265.
\[20\]石连旋，胡勇军，宫亮，等. 不同盐碱化草甸羊草越冬根茎中可溶性糖和蛋白的研究. 东北师大学报，2008，40（2）：8892.
\[21\]吴家红，靳凤云，龙开杰，等. 分光光度法测定板蓝根中总氨基酸. 微量元素与健康研究，2006，23（5）：4546.
\[22\]葛江丽，石雷，谷卫彬，等. 盐胁迫条件下甜高粱幼苗的光合特性及光系统II功能调节. 作物学报，2007，33（8）：12721278.
\[23\]Tripathi S B，Gurumurthi K，Panigrahi A K，et al. Salinity induced changes in proline and betaine contents and synthesis in two aquatic macrophytes differing in salt tolerance. Biol Plant, 2007，51（1）：110115.
\[24\]廖岩，陈桂珠. 三种红树植物对盐胁迫的生理适应. 生态学报， 2007，27（6）：22082214.
\[25\]李倩，刘景辉，武俊英，等. 盐胁迫对燕麦质膜透性及Na+、K+吸收的影响. 华北农学报，2009,24（6）：8892.
\[26\]魏国强，朱祝军，方学智，等. NaCl胁迫对不同品种黄瓜幼苗生长、叶绿素荧光特性和活性氧代谢的影响. 中国农业科学，2004，37（11）：17541759.
\[27\]杨志莹，赵兰勇，徐宗大. 盐胁迫对玫瑰生长和生理特性的影响. 应用生态学报，2011，22（8）：19931998.
\[28\]Hasegawa P M，Bressan R A，Pardo J M. The putative plasma membrane Na+/H+ antiporter SOS1 controls longdistance Na+ transport in plants. Trends Plant Sci, 2000，5：317319.
\[29\]Parida A K，Das A B. Salt tolerance and salinity effects on plants: A review. Ecotoxicol Environ Safety，2005，60：324349.
\[30\]Negrao S，Courtois B，Ahmadi N, et al. Recent updates on salinity stress in rice: From physiological to molecular responses. Plant Sci，2011，30：329377.
