粳稻8个异交相关性状及其中亲优势的QTL定位与遗传分析

张红1 ,江建华1,2 ,刘晓丽1 ,吉家曾1 ,牛付安1 ,张有富1,3 ,洪德林1,*

doi:10.3969/j.issn.10017216.2013.03.004

中国水稻科学 >

2013 , Vol. 27 >Issue 3: 247 - 258

DOI: https://doi.org/10.3969/j.issn.10017216.2013.03.004

研究报告

粳稻8个异交相关性状及其中亲优势的QTL定位与遗传分析

展开

¹南京农业大学作物遗传与种质创新国家重点实验室, 江苏南京210095; ²安徽省农业科学院作物研究所, 安徽合肥 230031; ³河西学院农学系，甘肃张掖 734000;

收稿日期: 2012-08-09

修回日期: 2012-09-22

网络出版日期: 2013-05-10

基金资助

国家863计划资助项目(2010AA101301)。

收起

QTL Mapping and Genetic Analysis of Eight Outcrossingrelated Traits and Its Midparental Heterosis in japonica Rice

Expand

¹ State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China; ²Institute of Crops, Anhui Academy of Agricultural Sciences, Hefei 230031, China;³ Department of Agronomy,Hexi College,Zhangye 734000, China;

Received date: 2012-08-09

Revised date: 2012-09-22

Online published: 2013-05-10

Fold

摘要

为探明控制粳稻异交相关性状及其中亲优势的基因作用类型，利用秀堡RIL群体及其2个回交(BCF1)群体对穗抽出度、剑叶长、剑叶角度、穗剑高度差、倒2叶长、倒2叶角度、穗与倒2叶（穗二）高度差和倒1节间长8个异交相关性状及其中亲优势进行QTL定位。3个群体中共检测到45个显著的主效QTL（MQTL），单个MQTL的贡献率变幅为1.5%～803%。73.3%的MQTL表现为加性效应，4.5%的MQTL表现为部分或完全显性效应，22.2%的MQTL表现为超显性效应。3个群体中共检测到82对显著的双基因上位性QTL（EQTL）。RIL群体中检测到43对EQTL，单对EQTL的贡献率变幅为1.0%～7.0%，平均2.7%。在以秀水79为父本、与秀堡RIL群体回交的后代（XSBCF1群体）中检测到16对EQTL，其中利用BCF1表型值检测到11对EQTL，单对EQTL的贡献率变幅为11.2%～36.8%，平均21.0%；利用中亲优势值检测到6对EQTL，单对EQTL的贡献率变幅为33.1%～76.8%，平均55.0%。在以C堡为父本、与秀堡RIL群体回交的后代（CBBCF1群体）中检测到23对EQTL，其中利用BCF1表型值检测到16对EQTL，单对EQTL的贡献率变幅为6.2%～60.0%，平均24.0%；利用中亲优势值检测到7对EQTL，单对EQTL的贡献率变幅为21.3%～44.4%，平均31.0%。上述结果表明，粳稻异交相关性状是由多位点控制的，基因对性状本身的作用类型以加性效应为主；粳稻异交相关性状中亲优势主要遗传基础为超显性效应和上位性效应。

关键词： 粳稻; 异交性状; 主效QTL; 上位性QTL

本文引用格式

张红1 ,江建华1,2 ,刘晓丽1 ,吉家曾1 ,牛付安1 ,张有富1,3 ,洪德林1,* . 粳稻8个异交相关性状及其中亲优势的QTL定位与遗传分析[J]. 中国水稻科学, 2013 , 27(3) : 247 -258 . DOI: 10.3969/j.issn.10017216.2013.03.004

Abstract

To understand the types of gene action controlling outcrossrelated traits and its midparental heterosis, QTL mapping of eight outcrossingrelated traits including panicle exsertion degree (PED), flag leaf length (FLL), flag leaf angle (FLA), vertical distance between panicle tip and flag leaf tip (DPFT), second leaf length (SLL), second leaf angle (SLA), vertical distance between panicle tip and second leaf tip (DPST) and length of the first internode (LFI) from the top was conducted using 254 Xiushui 79/C Bao recombinant inbred lines (RILs) and two backcross hybrid populations derived from these RILs. 45 maineffect QTLs (MQTL) were identified in the three populations. The percentage of phenotypic variance explained by each QTL ranged from 1.5% to 80.3%. Among them, 73.3% QTL showed an additive effect, 4.5% a partialtocomplete dominant effect, and 22.2% an overdominant effect. Eightytwo pairs of digenic epistatic QTL (EQTL) were detected in the 3 populations,of which 43 pairs were detected in RIL population, and the percentage of phenotypic variance explained by each pair of QTL ranged from 1.0% to 7.0%, with an average 2.7%. Sixteen pairs of EQTL were detected in XSBCF1 population. Among them, 11 pairs were detected by using XSBCF1 phenotypic value, and the percentage of phenotypic variance explained by each EQTL ranged from 11.2% to 36.8%, with an average 21.0%. Six pairs of EQTL were detected by using midparental heterosis value (HMP), and the percentage of phenotypic variance explained by each EQTL ranged from 33.1% to 76.8%, with an average 55.0%. Twentythree pairs of EQTL were detected in CBBCF1 population. Among them, 16 pairs of EQTL were detected by using BCF1 phenotypic value, and the percentage of phenotypic variance explained by each pair of EQTL ranged from 6.2% to 60.0%, with an average 24.0%. Seven pairs of EQTL were detected by using the midparental heterosis value, and the percentage of phenotypic variance explained by each pair of EQTL ranged from 21.3% to 44.4%, with an average 31.0%. These results showed that outcrossingrelated traits themselves were mainly controlled by multiple loci with additive effects, and the primary genetic basis of midparent heterosis for outcrossingrelated traits was overdominant and epistatic effect in japonica rice.

Key words： japonica rice; outcrossrelated trait; maineffect QTL; epistatic QTL

参考文献

\[1\]邓华凤, 何强, 舒服, 等. 中国杂交粳稻研究现状与对策. 杂交水稻, 2006, 21(1): 16.

\[2\]汤述翥, 张宏根, 梁国华, 等. 三系杂交粳稻发展缓慢的原因及对策. 杂交水稻, 2008, 23(1): 15.

\[3\]汤玉庚, 王才林. 再论长江流域杂交粳稻育种攻关中的几个问题. 杂交水稻, 1990(6): 36.

\[4\]洪德林, 汤玉庚. 粳稻雄性不育恢复基因研究:Ⅰ.粳稻雄性不育恢复基因的地理分布. 江苏农业学报, 1985, 1(4): 15.

\[5\]江建华, 赵其兵, 刘强明, 等. 利用条件QTL定位发掘粳稻生育期和株高及单株有效穗数适用有利等位变异. 中国水稻科学, 2011, 25(3): 277283.

\[6\]洪德林. 粳稻BT型同质恢和非同质恢育性恢复力及后代经济性状研究. 江苏农业科学，1998(5): 27.

\[7\]胡文德, 孙大运, 江建华, 等. 100份水稻资源对外源GA3敏感性比较. 南京农业大学学报, 2010, 33(6): 712.

\[8\]吉立柱, 陆作楣. 粳稻二元不育系开花习性的研究. 天津农业科学, 2002, 8(1): 1417.

\[9\]乔保建, 朱晓彪, 王盈盈, 等. 不同生长环境下水稻穗抽出度三个相关性状QTL定位研究. 作物学报, 2008, 34(3): 389396.

\[10\]杨德卫, 朱镇, 张亚东, 等. 基于CSSL的水稻穗颈长度QTL的代换作图. 遗传, 2009, 31(7): 741747.

\[11\]Luo A, Qian Q, Yin H, et al. EUI1, encoding a putative cytochrome P450 monooxygenase, regulates internodes elongation by modulating gibberellin responses in rice. Plant Cell Physiol, 2006, 47(2): 181191.

\[12\]Zhu Y, Nomura T, Xu Y, et al. ELONGATED UPPE RMOST INTERNODE encodes a cytochrome P450 monooxygenase that epoxidizes gibberellins in a novel deactivation reaction in rice. Plant Cell, 2006, 18(2): 442456.

\[13\]王一平, 曾建平, 郭龙彪, 等. 水稻顶部三叶与穗重的关系及其QTL分析. 中国水稻科学, 2004, 19(1): 1320.

\[14\]李兰芝, 卢开阳, 牟同敏, 等. 水稻剑叶和倒二叶形态性状的杂种优势. 武汉大学学报, 2008, 54(4): 472478.

\[15\]罗伟, 胡江, 孙川, 等. 水稻抽穗期功能叶叶型相关性状遗传分析. 分子植物育种, 2008, 6(5): 853860.

\[16\]王智权, 刘喜, 江玲, 等. 利用染色体片段置换系(CSSLs)群体检测水稻剑叶形态性状QTL. 南京农业大学学报, 2010, 33(6): 16.

\[17\]杨窑龙, 饶玉春, 李赓觅, 等. 水稻茎秆相关性状遗传分析. 分子植物育种, 2011, 9(2): 160168.

\[18\]Yan J, Zhu J, He C, et al. Molecular markerassisted dissection of genotype×environment interaction for plant type traits in rice (Oryza sativa L.). Crop Sci, 1999, 39(2): 538544.

\[19\]Kobayashi S, Fukuta Y, Morita S, et al. Quantitative trait loci affecting flag leaf development in rice (Oryza sativa L.). Breeding Sci, 2003, 53: 255262.

\[20\]胡文德, 张红, 江建华, 等. 粳稻剑叶斜下伸资源的发现及剑叶角度的分离分析和QTL定位. 中国水稻科学, 2012, 26 (1): 3442.

\[21\]Wang L, Xu Y, Zhang C, et al. OsLIC, a novel CCCHType zinc finger protein with transcription activation, mediates rice architecture via brassinosteroids signaling. PLoS ONE, 2008, 3 (10): e3521.

\[22\]喻婷, 张玲, 胡中立, 等. 水稻籼粳交DH群体花器性状的遗传分析. 武汉植物学研究, 2003, 21 (6): 459463.

\[23\]Mei H W, Li Z K, Shu Q Y, et al. Gene actions of QTLs affecting several agronomic traits resolved in a recombinant inbred rice population and two backcross populations. Theor Appl Genet, 2005, 110: 649659.

\[24\]牛付安, 刘健, 郭媛, 等. 4个环境下稳定表达的控制粳稻穗角性状的新位点. 中国水稻科学, 2012, 26 (4): 409416.

\[25\]Wang D L, Zhu J, Li Z K, et al. Mapping QTLs with epistatic effects and QTL×environment interactions by mixed linear model approaches. Theor Appl Genet, 1999, 99: 12551264.

\[26\]Mather K, Jinks J L. Biometrical Genetics, 3rd edn. London: Chapman and Hall, 1982.

\[27\]McCouch S R. Gene nomenclature system for rice. Rice, 2008, 1（1）: 7284.

\[28\]江建华, 刘强明, 卢超, 等. 粳稻杂种优势遗传基础剖析. 作物学报, 2012, 38(12): 21472161.

\[29\]林鸿宣，庄杰云，等. 水稻株高及其构成因素数量性状基因座位的分子标记定位.作物学报, 1996, 22 (3) : 257263.

Options

文章导航

模态框（Modal）标题

摘要

本文引用格式

Abstract

参考文献