抑制OsAGO1a基因的表达导致水稻叶片近轴面卷曲

李磊1,#,薛芗1,#,左示敏1,陈宗祥1,张亚芳1,李前前1,朱俊凯2,马玉银3,潘学彪1,*,潘存红2,*

doi:10.3969/j.issn.10017216.2013.03.001

中国水稻科学 >

2013 , Vol. 27 >Issue 3: 223 - 230

DOI: https://doi.org/10.3969/j.issn.10017216.2013.03.001

研究报告

抑制OsAGO1a基因的表达导致水稻叶片近轴面卷曲

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¹扬州大学农学院, 江苏扬州 225009; ²江苏里下河地区农业科学研究所, 江苏扬州 225007; ³扬州职业大学生化工程系, 江苏扬州 225009;

收稿日期: 2012-12-13

修回日期: 2013-01-24

网络出版日期: 2013-05-10

基金资助

国家自然科学基金资助项目（31201180）；江苏省自然科学基金资助项目（BK2008223；BK2012690）。

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Suppressed Expression of OsAGO1a Leads to Adaxial Leaf Rolling in Rice

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¹ Agricultural College, Yangzhou University, Yangzhou 225009, China; ² Lixiahe Agricultural Research Institute of Jiangsu Province, Yangzhou 225007, China; ³ Department of Biochemical Engineering, Yangzhou Polytechnic College, Yangzhou 225009, China; #These authors contribute equally to this paper;

Received date: 2012-12-13

Revised date: 2013-01-24

Online published: 2013-05-10

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摘要

AGO蛋白是RNA诱导沉默复合体的核心元件，在植物生长发育过程中发挥着重要作用。通过分别特异性地扩增OsAGO1a基因中第2外显子和第11~12外显子的片段，构建了两个干涉载体OsAGO1aI1E和OsAGO1aI2E。利用农杆菌介导法转化水稻品种日本晴，抑制OsAGO1a基因的表达，借此研究OsAGO1a的功能及其对水稻生长发育的影响。结果表明, 抑制OsAGO1a的表达导致叶片的近轴面卷曲而对其他主要农艺性状的影响不大。

关键词： 水稻; OsAGO1a; 极性发育; 卷叶; 农艺性状

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李磊1,#,薛芗1,#,左示敏1,陈宗祥1,张亚芳1,李前前1,朱俊凯2,马玉银3,潘学彪1,*,潘存红2,* . 抑制OsAGO1a基因的表达导致水稻叶片近轴面卷曲[J]. 中国水稻科学, 2013 , 27(3) : 223 -230 . DOI: 10.3969/j.issn.10017216.2013.03.001

Abstract

Argonaute (AGO) protein is the core effectors in RNAinduced silencing complexes (RISCs), playing an important role in plant development. The 2nd and the 11th-12th exon fragments of OsAGO1awere amplified independently to make two RNAi constructs: OsAGO1aI1E and OsAGO1aI2E. To clarify the function of OsAGO1a in rice development, the expression of OsAGO1a has been suppressed by introducing RNAi constructs to Nipponbare through Agrobacteriummediated transformation. The result showed that the reduced expression of OsAGO1a lead to adaxial leaf rolling without affecting main agronomic traits.

Key words： rice; OsAGO1a; polarity development; rolled leaf; agronomic traits

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