以6个籼型三系杂交稻不育系和5个恢复系按不完全双列杂交配制的30个杂交稻组合及其亲本为材料,对其淀粉RVA谱特征值进行了测定和分析。结果表明:淀粉RVA谱特征值在各组合间均存在极显著遗传差异,其加性效应和非加性效应对杂交稻淀粉RVA谱特征值均存在极显著的影响,并以加性效应为主;杂交稻亲本RVA谱特征值一般配合力(GCA)效应高的,其后代特殊配合力(SCA)效应不一定高;杂交稻母本RVA谱特征值GCA方差在总的基因型方差中所占比重远远大于父本和组合SCA方差,表明杂交稻淀粉RVA谱特征值主要受不育系的影响;杂交稻淀粉RVA谱特征值的竞争优势与母本的GCA极显著正相关,且决定系数较高,表明杂交稻母本的一般配合力高,其所配杂交稻组合的竞争优势也强;杂交稻淀粉RVA谱特征值的峰值黏度存在明显的超高亲优势;糊化开始温度存在超低亲优势;其他性状存在中亲优势。
To evaluate the heterosis of rice starch viscosity properties, 30 hybrid combinations were obtained from incomplete diallel cross between six sterile lines and five restorer lines of indica hybrid rice cultivars. The rapid viscosity analyzer (RVA) profile parameters of starch viscosity in hybrid combinations and parents were measured and statistically analyzed. Results showed that there were highly significant genetic differences for RVA properties among the hybrid rice combinations. The starch viscosity properties in hybrid rice combinations were highly significantly affected by gene additive effects and nonadditive effects, majorly affected by parent gene additive effects. The specific combining ability (SCA) of offspring hybrid rice combination was not always high, though the parents have high general combining ability (GCA). The proportion of female parent GCA variance was far bigger than both the proportion of male parent GCA variance and proportion of combinations SCA variance, indicating that the starch viscosity properties in hybrid rice combinations were mainly affected by the sterile lines. There were not only highly significant positive correlations between the competitive advantages of the hybrid rice combinations and female parents′ GCA but also higher determination coefficients, suggesting that the hybrid combinations from female parents with higher GCA have higher competitive advantages. We also found that highly significant over highparent heterosis in peak viscosity, highly significant negative below lowparent heterosis in pasting temperature and highly significant positive over midpatent heterosis in other starch viscosity characteristics.
\[1\]Deffenbaugh L B, Walker C E. Determination of amylose content and its relationship with RVA profile within genetically similar cultivars of rice(Oryza sativa ssp.japonica). Agric Sci China, 2010(8): 11011107.
\[2\]Bason M L. Assessing rice quality using the RVAResults of an international collaborative trial. RVA World, 1994(6): 25.
\[3\]Redy K R, Subramanian R, Ali S Z, et al. Viscoelastic properties of rice flour pastes and their relationship to amylose and rice quality.Cereal Chem, 1994,71: 548552.
\[4\]王肇慈.稻米食用、蒸煮品质评价与分析粮油品质分析.北京: 中国轻工业出版社,1997: 170.
\[5\]吴殿星, 舒庆尧, 夏英武.RVA分析辅助选择食用优质早籼稻的研究.作物学报, 2001, 27(2): 165172.
\[6\]王丰, 程方民, 钟连进, 等.早籼稻米RVA谱特性的品种间差异及其温度效应特征.中国水稻科学, 2003, 17(4): 3943.
\[7\]包劲松, 舒庆尧, 夏英武, 等.γ辐照对稻米蒸煮和食用品质的影响.核农学报, 1998, 12(6): 327331.
\[8\]Bao J S, Shen S Q, Xia Y W. Analysis of genotype × environment interaction effects for starch pasting viscosity characteristics in indica rice. Acta Gene Sin, 2006, 33(11): 10071013.
\[9\]Zheng L N, Zhang W W, Liu S J, et al. Genetic relationship between grain chalkiness, protein content, and paste viscosity properties in a backcross inbred population of rice. J Cereal Sci, 2012, 56(2):153160.
\[10\]李刚, 邓其明, 李双成, 等.稻米淀粉RVA谱特征与品质性状的相关性.中国水稻科学, 2009, 23(1): 99102.
\[11\]Han Y P, Xu M L, Liu X Y, et al. Genes coding for starch branching enzymes are major contributors to starch. Plant Sci, 2004, 166(2): 257364.
\[12\]焦桂爱, 胡培松, 唐绍清, 等.香稻品种RVA谱多样性研究.核农学报, 2010,24(1): 7882.
\[13\]张永生, 江玲, 刘喜, 等.优质水稻品种越光(Koshihikari)中控制稻米淀粉RVA谱特征值的QTL分析.中国水稻科学, 2010, 24(2): 137144.
\[14\]Zhang Q F, Zhang Y D, Zhu Z, et al. Inheritance analysis and QTL mapping of rice starch viscosity (rapid visco analyzer profile) characteristics. Rice Sci, 2008, 15(3): 186194.
\[15\]金正勋, 姜文洙, 晋重玄, 等.籼稻品种味度及淀粉RVA谱特性配合力分析.作物学报, 2004, 30(12): 12101214.
\[16\]Zhou K D, Li H Y , Li R D. Preliminary study on the combining ability and heritability of main characteristics of hybrid rice. Acta Agron Sin, 1982, 8(3): 145151.
\[17\]黄远樟, 刘来福. 作物数量遗传学基础. 遗传, 1980, 2(2): 4346.
\[18\]Gravois K A, Webb B D. Inheritance of long grain rice amylograph viscosity characteristics. Euphytica, 1997, 97: 2529.
\[19\]Bao J S, Corke H, He P, et al. Analysis of quantitative trait LOCI for starch properties of rice based on an RIL population. Acta Bot Sin, 2003, 45(8): 986994.
\[20\]洪德林, 杨开晴, 潘恩飞.粳稻不同生态类型间F1的杂种优势及其亲本的配合力分析.中国水稻科学, 2002,16(3): 1923.
\[21\]伍箴勇, 李春海, 牟同敏.两系杂交籼稻配合力分析.中国水稻科学, 2007, 21(6): 612618.
\[22\]陆芳芳, 陆卫平, 刘萍, 等.糯玉米淀粉RVA黏度的杂种优势分析.作物学报, 2006, 32(4): 503508.