云南元阳哈尼梯田水稻地方品种月亮谷的遗传变异分析

董超1,#,徐福荣1,#,杨文毅1 ,汤翠凤1 ,张恩来1 ,杨雅云1 ,阿新祥1 ,张斐斐1,卢光德2,王艳2 ,戴陆园1,*

doi:10.3969/j.issn.10017216.2013.02.005

中国水稻科学 >

2013 , Vol. 27 >Issue 2: 137 - 144

DOI: https://doi.org/10.3969/j.issn.10017216.2013.02.005

研究报告

云南元阳哈尼梯田水稻地方品种月亮谷的遗传变异分析

展开

¹云南省农业科学院生物技术与种质资源研究所/云南省农业生物技术重点实验室/农业部西南作物基因资源与种质创制重点实验室/农业部云南稻种资源科学观测实验站，云南昆明 650223；²云南省元阳县农业局，云南元阳 662414；

收稿日期: 2012-05-02

修回日期: 2012-08-21

网络出版日期: 2013-03-10

基金资助

国家863计划资助项目（2010AA101805）；云南省科技创新强省计划资助项目(2007C0219Z)；云南省社会发展科技计划项目(2010CC009; 2012CH009)；云南省人才培养资助项目(2008PY049)。

收起

Genetic Variation Analysis of Paddy Rice Landrace of Yuelianggu from Yuanyang Hani’s Terraced Fields in Yunnan Province

Expand

¹Biotechnology and Germplasm Resources Institute, Yunnan Academy of Agricultural Sciences /Yunnan Provincial Key Laboratory of Agricultural Biotechnology/ Key Laboratory of Southwestern Crop Gene Resources and Germplasm Innovation, Ministry of Agriculture/ Scientific Observation for Rice Germplasm Resources of Yunnan, Ministry of Agriculture, Kunming 650223, China; ²Yuanyang Department of Agriculture,Yuanyang 662414, China;

Received date: 2012-05-02

Revised date: 2012-08-21

Online published: 2013-03-10

Fold

摘要

为了揭示云南元阳哈尼梯田持续大面积种植的水稻地方品种月亮谷的遗传变异，利用20个表型性状和48个SSR标记，对采自元阳哈尼梯田11个村寨的98份月亮谷和8份对照品种进行分析。结果显示，98份月亮谷的质量性状变异系数较小；数量性状变异系数较大，变异系数范围为2.55%~24.90%；48个标记共检测出114个等位变异，平均为2.38个，变幅为1~4个；有效等位变异为87.65个，平均为1.83个，变幅为1~3.02；Nei遗传多样性（I）指数平均为0.64。表型聚类将月亮谷与对照品种分成两大类，所有月亮谷为一类，8个对照品种为另一类；SSR聚类则将所有月亮谷与4份籼型对照品种聚为一大类，4份粳型对照品种单独为一大类；分子方差分析（AMOVA）表明，月亮谷村寨之间变异（67.18%）大于村寨内（37.82%）。11个村寨月亮谷可以分成5个居群，这5个类群与其村寨间地理位置的距离相关，相邻村寨之间月亮谷遗传距离小，遗传相似度高。月亮谷这种特殊的遗传变异是由于独特的哈尼梯田及其传统文化长期作用的结果，月亮谷可作为优异基因资源进一步发掘和利用。

关键词： 哈尼梯田; 水稻; 遗传变异; 月亮谷

本文引用格式

董超1,#,徐福荣1,#,杨文毅1 ,汤翠凤1 ,张恩来1 ,杨雅云1 ,阿新祥1 ,张斐斐1,卢光德2,王艳2 ,戴陆园1,* . 云南元阳哈尼梯田水稻地方品种月亮谷的遗传变异分析[J]. 中国水稻科学, 2013 , 27(2) : 137 -144 . DOI: 10.3969/j.issn.10017216.2013.02.005

Abstract

In order to reveal the genetic variation of rice landrace Yuelianggu which has been widely grown in Yunnan Hani’s terraced fields for longterm,98 Yuelianggu samples collected from 11 villages located in Yunnan and 8 rice varieties as control were analyzed by using 20 phenotypic traits and 48 SSR markers. The results showed that the variation coefficients for the quantitative characters were larger than those of the qualitative characters with a range from 2.55% to 24.90%. A total of 114 alleles in all of Yuelianggu were found with the mean of 2.38 and range from 1 to 4, as well as the number of 87.65 effective alleles with the mean of 1.83 and range from 1 to 3.02. The mean of Nei’s genetic diversity index (I) was 0.64. Clustering analysis based on phenotypes could divide the used materials including Yuelianggu and control varieties into two categories, with Yuelianggu belonging to one group while the other eight varieties to another one. However, clustering analysis based on SSR markers could divide the tested moterials into two categories, with one group including all Yuelianggu samples and the indica control while another one only including the japonica control. Analysis of molecular variance （AMVOA） revealed that the intervillage genetic variations of Yuelianggu consisted of 67.18% and the intravillage genetic variation contisted of 37.82%. Yuelianggu samples collected from 11 villages could be divided into 5 populations, which were closely related to geographical distance between the villages. Genetic distances of Yuelianggu collected from adjacent villages were small while their genetic similarities were high correspondly. The special genetic variation of Yuelianggu resulted from the unique Hani’s terraced fields and its traditional culture for longterm. Yuelianggu can be used as a benefit gene resource to further exploit and utilize.

Key words： Hani’s terraced field; rice; genetic variation; Yuelianggu

参考文献

\[1\]Zhu Y Y, Chen H R, Fan J H, et al. Genetic diversity and disease control in rice. Nature, 2000, 406(6797): 718722.

\[2\]齐永文, 张冬玲, 张洪亮, 等.中国水稻选育品种遗传多样性及其近50年变化趋势.科学通报, 2006, 51(6): 693699.

\[3\]魏兴华, 汤圣祥, 江云珠, 等.中国栽培稻选育品种等位酶多样性及其与形态学性状的相关分析.中国农业科学, 2003, 17(2):123128.

\[4\]林世成, 闵绍楷.中国水稻及其系谱.上海：上海科学技术出版社, 1992： 282292.

\[5\]庄杰云, 钱惠荣, 陆军, 等.籼稻品种遗传变异性初探.中国农业科学, 1996, 29(2): 1722.

\[6\]郑康乐, 庄杰云.我国水稻推广品种的遗传变异性和种质资源的开发利用.中国农业科技导报, 2000, 2(3): 6972.

\[7\]曾亚文, 李自超, 申时全, 等.云南地方稻种的多样性及优异种质研究.中国水稻科学, 2001, 15(3): 169174.

\[8\]Dai L Y, Lin X H, Ye C R, et al. Identification of quantitative trait loci controlling cold tolerance at the reproductive stage in Yunnan landrace of rice Kunmingxiaobaigu. Breeding Sci, 2004, 54: 253258.

\[9\]杨志奇, 杨春刚, 汤翠凤, 等.中国粳稻地方品种孕穗期耐冷性评价及聚类分析.植物遗传资源学报, 2008, 9(4): 485491.

\[10\]卢宝荣, 朱有勇, 王云月.农作物遗传多样性农家保护的现状及前景.生物多样性, 2002, 10(4): 409414.

\[11\]高东, 王云月, 何霞红, 等.元阳白脚老粳水稻地方品种内遗传异质性及意义.分子植物育种, 2009, 7(2): 283291.

\[12\]高东, 毛如志, 朱有勇.水稻地方品种与改良品种内部遗传异质性的比较分析.分子植物育种, 2010, 8(3): 432438.

\[13\]孙建昌, 曹桂兰, 李亚非, 等.水稻地方品种群体内的遗传多样性分析.西北农林科技大学学报：自然科学版, 2011, 12(39): 19.

\[14\]董树斌, 卢宝荣, 王云月, 等.云南水稻传统品种内的遗传多样性及其维持机制初探.云南农业大学学报, 2010, 15(1): 18.

\[15\]王清华.袁牢山自然生态与哈尼族生存格局. 昆明：云南社会科学出版社, 1998, 2: 7174.

\[16\]王清华.梯田文化论. 昆明：云南大学出版社, 1999： 1315.

\[17\]徐福荣, 张恩来, 董超, 等.云南元阳哈尼梯田两个不同时期种植的水稻地方品种表型比较.生物多样性, 2010, 18(4):365372．

\[18\]徐福荣, 董超, 杨文毅, 等.利用微卫星标记比较云南元阳哈尼梯田两个不同时期种植的水稻地方品种的遗传多样性. 中国水稻科学, 2011, 25(4): 381386.

\[19\]杨文毅.元阳哈尼梯田水稻地方品种“月亮谷”的遗传差异研究\[D\]. 昆明：云南农业大学, 2011： 78.

\[20\]He X H, Sun Y, Gao D, et al.Comparison of agronomic traits between rice landraces and modern varieties at different altitudes in the paddy fields of Yuanyang Terrace, Yunnan Province. J Resour Ecol, 2011, 2(1): 4650.

\[21\]徐福荣, 张恩来, 董超, 等.云南哈尼梯田地方稻种主要农艺性状鉴定评价.植物遗传资源学报, 2010, 11(4): 413417.

\[22\]杨庆文, 陈成斌, 张万霞, 等.SSR等位变异数对普通野生稻居群遗传结构分析的影响.中国水稻科学, 2005, 19(4):297302.

\[23\]魏兴华, 袁筱萍, 余汉勇, 等.我国常规稻主栽品种的遗传变异分析.中国水稻科学, 2009, 23(3): 237244.

\[24\]彭锁堂, 王海岗, 魏兴华, 等.我国三系杂交稻主要不育系的微卫星标记多样性和遗传结构分析.中国水稻科学, 2008, 22(4): 365369.

\[25\]韩龙植, 魏兴华, 曹桂兰, 等.水稻种质资源描述规范和数据标准.北京：中国农业出版社, 2006： 1525.

\[26\]Edwards K, Johnstone C, Thompson C.A simple and rapid method for the preparation of plant genomic DNA for PCR analysis. Nucleic Acids Res, 1991, 19: 4349.

\[27\]陈雨, 潘大建, 曲延英, 等.水稻籼粳分化研究进展.广东农业科学, 2007(12):37.

\[28\]李月成. 云南稻作栽培//蒋志农. 云南稻作. 昆明：云南科学技术出版社, 1995： 254258.

\[29\]黄建勋, 张凯, 江良荣, 等.SSR标记对籼稻品种的遗传多样性分析. 厦门大学学报：自然科学版, 2006, 45(1): 120124.

\[30\]樊叶杨, 庄杰云, 吴建利, 等.应用微卫星标记鉴别水稻籼粳亚种.遗传, 2000, 22(6): 392394.

\[31\]徐福荣.云南元阳哈尼梯田水稻地方品种遗传多样性研究\[D\]. 南京：南京农业大学, 2010： 6668.

\[32\]高东, 何霞红, 朱有勇.元阳水稻地方品种多样性变化及换种规律研究.植物遗传资源学报, 2011, 12(2): 311313.

\[33\]严红梅, 李进斌, 汤翠凤, 等.云南元阳哈尼梯田不同时期种植的地方品种稻瘟病抗性评价.中国农学通报, 2011, 27(15): 263268.

\[34\]陆旭, 杨志新, 续勇波.不同氮肥用量对元阳梯田水稻产量及构成因素的影响.云南农业大学学报, 2011, 26(3): 376381.

\[35\]毛佑全, 李期博.哈尼族. 北京：民族出版社, 1989： 6365.

\[36\]孙官生.古老神奇博大—哈尼族文化探源. 昆明：云南人民出版社, 1991： 2727.

\[37\]角媛梅.哈尼梯田文化生态系统研究.人文地理(增), 1999： 5659.

\[38\]姚敏, 崔保山.哈尼梯田湿地生态系统的垂直特征.生态学报, 2006, 26(7): 21152124.

Options

文章导航

模态框（Modal）标题

摘要

本文引用格式

Abstract

参考文献