水稻类树状突变体pla15的鉴定与基因克隆

封功能1,2,张昌泉1,赵冬生1,朱孔志1,涂怀洲1,徐辰武1,刘巧泉1，*

doi:10.3969/j.issn.10017216.2013.02.001

中国水稻科学 >

2013 , Vol. 27 >Issue 2: 111 - 116

DOI: https://doi.org/10.3969/j.issn.10017216.2013.02.001

研究报告

水稻类树状突变体pla15的鉴定与基因克隆

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¹扬州大学农学院教育部植物功能基因组学重点实验室/江苏省作物遗传生理重点实验室，江苏扬州225009；²盐城工学院，江苏盐城 224051；

收稿日期: 2012-07-30

修回日期: 2012-09-20

网络出版日期: 2013-03-10

基金资助

江苏省杰出青年基金资助项目（BK2012010）；教育部科学技术研究重点资助项目。

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Identification and Gene Cloning of the Leafy Head Mutant pla15 in Rice

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¹Key Laboratory of Plant Functional Genomics of the Ministry of Education/Key Laboratory of Crop Genetics and Physiology of Jiangsu Province, College of Agriculture, Yangzhou University, Yangzhou 225009, China; ² Yancheng Institute of Technology, Yancheng 224051, China;

Received date: 2012-07-30

Revised date: 2012-09-20

Online published: 2013-03-10

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摘要

在粳稻品种台北309经60Coγ辐射诱变的后代中发现了一份类树状突变体pla15，该突变体表现为植株矮化、叶片小且数量增多、分蘖数减少、高位分蘖、穗分化受阻等特征。遗传分析表明，该性状受一对隐性核基因控制。利用图位克隆技术将目的基因定位在第10染色体长臂上两个分子标记CHR1027和CHR1030之间，物理距离为58 kb，并与SSR标记CHR1028和CHR1029共分离。根据水稻基因组序列的注释，该区域内存在5个完整的预测基因。测序分析表明pla15突变体中一个编码细胞色素P450 CYP78A11的释义基因LOC_Os10g26340第1外显子内缺失了一个碱基T，导致移码突变和翻译提前终止。据此，初步推测细胞色素P450 CYP78A11基因为PLA15的候选基因。

关键词： 水稻; 类树状突变体pla15; 遗传分析; 基因克隆; P450 CYP78A11

本文引用格式

封功能1,2,张昌泉1,赵冬生1,朱孔志1,涂怀洲1,徐辰武1,刘巧泉1，* . 水稻类树状突变体pla15的鉴定与基因克隆[J]. 中国水稻科学, 2013 , 27(2) : 111 -116 . DOI: 10.3969/j.issn.10017216.2013.02.001

Abstract

From the progeny of a japonica variety Taipei 309 treated with 60Coγ ray irradiation, a leafy head mutant pla15 (plasttochron 15) with dwarf phenotype and small leaf was identified. Comparing with the wild type, the pla15 mutant has more leaves and less tillers, and therefore fails to produce a normal panicle at the maturity stage. The genetic analysis showed that the pla15 phenotype was controlled by a single recessive nuclear gene. By the mapbased cloning, the target gene was finally narrowed down to a 58 kb region between SSR markers CHR1027 and CHR1030 on the long arm of chromosome 10, and cosegregates with the molecular markers CHR1028 and CHR1029. There were five predicted genes in the mapped region. The sequencing analysis results revealed that there was a deletion of the nucleotide T in the first exon of the gene LOC_Os10g26340 encoding cytochrome P450 CYP78A11 in the pla15 mutant, which might result in a downstream frame shift and a premature termination. These results implied that the P450 CYP78A11 gene might be the candidate gene of PLA15.

Key words： Oryza sativa L.; leafy head mutant pla15; genetic analysis; gene cloning; P450 CYP78A11

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