研究了水稻缺磷条件下蔗糖对其生长、磷素吸收利用的影响。结果表明，外加蔗糖可以提高水稻缺磷条件下的生物量，可以促进水稻缺磷条件下的磷素吸收；利用磷转运蛋白基因OsPT2启动子+ GUS报告基因转基因水稻材料，进一步探讨了蔗糖在水稻缺磷条件下促进磷素吸收利用的分子机理。结果表明，水稻磷转运蛋白OsPT2参与了蔗糖促进缺磷水稻的磷素吸收与转运。
The effects of sucrose on the growth, phosphorus uptake in rice under phosphate deficient conditions were studied. The results showed that exogenous sucrose can increase the biomass of rice, promote the absorption of phosphorus in rice under phosphate deficiency. Using phosphate transporter gene OsPT2 promoter +GUS reporter gene transgenic rice, we further explored the molecular mechanism that the uptake and use of phosphate was promoted in rice by sucrose under phosphate deficiency. The results showed that rice phosphate transporter OsPT2 was involved in this process.
\[1\]Raghothama K G. Phosphate acquisition.Ann Rev Plant Physiol Plant Mol Biol, 1999, 50(1): 665693.
\[2\]Dinkelaker B, Romheld V, Marschner H. Citric acid excretion and precipitation of calcium citrate in the rhizosphere of white lupin (Lupinus albus L.). Plant Cell Environ, 1989, 12: 285292.
\[3\]Mudge S R, Rae A L, Diatloff E,et al. Expression analysis suggests novel roles for members of the Pht1 family of phosphate transporters in Arabidopsis. Plant J, 2002, 31: 341353.
\[4\]Shin H, Shin H S, Dewbre G R, et al.Phosphate transport in Arabidopsis: Pht1;1 and Pht1;4 play a major role in phosphate acquisition from both low and highphosphate environments. Plant J, 2004, 39: 629642.
\[5\]Bucher M. Functional biology of plant phosphate uptake at root and mycorrhiza interfaces.New Phytol, 2007, 173: 1126.
\[6\]Rubio V, Linhares F, Solanor R, et al. A conserved MYB transcription factor involved in phosphate starvation signaling both in vascular plants and in unicellular algae.Genes Dev, 2001, 15: 21222133.
\[7\]Devaiah B N, Karthikeyan A S, Raghothama K G. WRKY75 transcription factor is a modulator of phosphate acquisition and root development in Arabidopsis. Plant Physiol, 2007, 143: 17891801.
\[8\]Devaiah B N, Nagarajan V K, Raghothama K G. Phosphate homeostasis and root development in Arabidopsis is synchronized by the zinc finger transcription factor ZAT6. Plant Physiol, 2007, 145: 147159.
\[9\]Devaiah B N, Madhuvantihi R, Karthikeyan A S, et al. Phosphate starvation responses and gibberellic acid biosynthesis are regulated by the MYB62 transcription factor in Arabidopsis, Mol Plant, 2009, 2: 4358.
\[10\]Chen Y F, Li L Q, Xu Q, et al. The WRKY6 transcription factor modulates PHOSPHATE1 expression in response to low pi stress in Arabidopsis. Plant Cell, 2009, 21: 35543566.
\[11\]Yi K, Wu Z, Zhou J, et al.OsPTF1, a novel transcription factor involved in tolerance to phosphate starvation in rice. Plant Physiol, 2005, 138: 20872096.
\[12\]Zhou J, Jiao F, Wu Z, et al.OsPHR2 is involved in phosphatestarvation signaling and excessive phosphate accumulation in shoots of plants. Plant Physiol, 2008, 146: 16731686.
\[13\]Hammond J P, White P J. Sugar signaling in root responses to low phosphorus availability.Plant Physiol, 2011，157（3）: 10331040.
\[14\]Karthikeyan A S, Varadarajan D K, Jain A, et al. Phosphate starvation responses are mediated by sugar signaling in Arabidopsis. Planta, 2007, 225: 907918.
\[15\]Wind J, Smeekens S, Hanson J. Sucrose: Metabolite and signaling molecule.Phytochemistry, 2010, 71: 16101614.
\[16\]Smeekens S, Ma J, Hanson J, et al. Sugar signals and molecular networks controlling plant growth.Curr Opin Plant Biol, 2010, 13: 274279.
\[17\]Hammond J P, White P J. Sucrose transport in the phloem: Integrating root responses to phosphorus starvation.J Exp Bot, 2008, 59(1): 93109.
\[18\]Turgeon R, Wolf S. Phloem transport: Cellular pathways and molecular trafficking.Annu Rev Plant Biol, 2009, 60: 207221.
\[19\]Liu J, Samac D A, Bucciarelli B, et al. Signaling of phosphorus deficiencyinduced gene expression in white lupin requires sugar and phloem transport.Plant J, 2005, 41: 257268.
\[20\]Ai P, Sun S, Zhao J, et al. Two rice phosphate transporters,ORYsa;Pht1;2 and ORYsa;Pht1;6, exhibit different kinetic properties and functions in uptake and translocation of phosphate. Plant J, 2009, 57: 798809.
\[21\]Jia H F, Ren H Y, Gu M, et al. Phosphate transporter gene, OsPht1;8, is involved in phosphate homeostasis in rice. Plant Physiol, 2011, 156: 11641175.
\[22\]Chen A Q, Hu, J, Sun, S B, et al. Conservation and divergence of both phosphate and mycorrhizaregulated physiological responses and expression patterns of phosphate transporters in solanaceous species.New Phytol, 2007, 173, 817831.
\[23\]Schunmann P H D, Richardson A E, Smith F W, et al. Characterization of promoter expression patterns derived from the Pht1 phosphate transporter genes of barley (Hordeum vulgare L.). J Exp Bot, 2005, 55: 855865.
