The RVA profile is one of the important factors for rice cooking and eating quality evaluation, and it would be of great significance to identify new quantitative trail loci (QTLs) for RVA profile for improvement of rice quality. With Nipponbare as recipient and 9311 as donor, a population with 38 chromosome segment substitution lines (CSSLs) had been developed and subsequently genotyped by highthroughput resequencing strategy. The population with their parents similar in apparent amylose content was used for mapping QTLs of RVA properties including peak paste viscosity (PKV), hot paste viscosity (HPV), cool paste viscosity (CPV), breakdown viscosity (BDV), setback viscosity (SBV), consistence viscosity (CSV), peak time (PeT) and pasting temperature (PaT) in two years and two environments. The QTL analysis was carried out by using oneway ANOVA and Dunnett’s test, and stable QTLs were identified in both two years and two environments. 10 stable QTLs, qPKV2.1, qPKV5.1, qPKV7.1, qPKV8.1, qHPV5.1, qHPV7.1, qCPV5.1, qCPV7.1, qSBV2.1 and qSBV7.1 were identified in the chromosome 2, 5, 7 and 8, respectively, with contributions ranging from -31.8% to 53.2%. The QTLs in chromosome 2, 5 and 7 were found to have pleiotropy.
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