基于SSR标记的云南地方稻种群体内遗传多样性分析

孙建昌1,2,余滕琼3,汤翠凤3,曹桂兰1,徐福荣3,韩龙植1,*

doi:10.3969/j.issn.10017216.2013.01.006

中国水稻科学 >

2013 , Vol. 27 >Issue 1: 41 - 48

DOI: https://doi.org/10.3969/j.issn.10017216.2013.01.006

研究报告

基于SSR标记的云南地方稻种群体内遗传多样性分析

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¹中国农业科学院作物科学研究所/国家农作物基因资源与基因改良重大科学工程/农业部作物种质资源利用重点开放实验室, 北京 100081; ²宁夏农林科学院农作物研究所, 宁夏永宁 750105；³云南省农业科学院生物技术与种质资源研究所,云南昆明 650205;

收稿日期: 2012-03-29

修回日期: 2012-08-30

网络出版日期: 2013-01-10

基金资助

国家科技支撑计划资助项目(2006BAD13B01)；农业部作物种质资源保护项目(NB201021301352501)。

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Analysis of Genetic Diversity within Populations of Rice Landraces from Yunnan Using Microsatellite Markers

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¹ Institute of Crop Sciences, Chinese Academy of Agricultural Sciences/Key Laboratory of Crop Germplasm Resources and Utilization, Ministry of Agriculture/National Key Facility for Crop Gene Resources and Genetic Improvement, Beijing 100081,China; ² Institution of Crop Research, Ningxia Academy of Agricultural Sciences, Yongning 750105, China; ³ Institute of Biotechnology and Germplasm Resources, Yunnan Academy of Agricultural Sciences, Kunming 650205, China;

Received date: 2012-03-29

Revised date: 2012-08-30

Online published: 2013-01-10

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摘要

用20对SSR引物对原产于云南的16份地方稻种和2份选育品种进行单个品种群体内的遗传多样性分析。结果表明，87.5%的地方品种群体内SSR多态性高于选育品种，而12.5%的地方品种群体内SSR标记多态性与选育品种相近。81.2%的地方稻种群体内的等位基因数(Na)和Nei基因遗传多样性指数(He)高于选育品种，而18.8%的地方品种群体内Na和He与选育品种相同或略小。水稻地方稻种群体内He的差异较大，其变异范围为0.0146～0.5117，其中，黄板所1（1980年收集）、黄板所2（2007年收集）、麻线谷1（1980年收集）、麻线谷2（2007年收集）的群体内遗传多样性较高，分别为0.2327、0.4214、0.5117和0.4489。87.5%地方品种的杂合度(Ho)明显高于选育品种；地方品种群体间遗传多样性差异很大，其中1/4的遗传差异来源于地方稻种群体内，差异极显著。RM333、RM257和RM180在供试云南地方稻种群体内的多态性、等位基因数、多样性指数和变异百分率均较高，适用于云南地方稻种群体内遗传多样性检测。

关键词： 水稻; 地方品种; 群体内遗传多样性; 微卫星标记

本文引用格式

孙建昌1,2,余滕琼3,汤翠凤3,曹桂兰1,徐福荣3,韩龙植1,* . 基于SSR标记的云南地方稻种群体内遗传多样性分析[J]. 中国水稻科学, 2013 , 27(1) : 41 -48 . DOI: 10.3969/j.issn.10017216.2013.01.006

Abstract

Genetic diversity within populations of 16 rice landraces and 2 improved cultivars from Yunnan Province were analyzed using 20 SSR markers. The results showed that the SSR marker polymorphism within populations for 87.5% of rice landraces was higher than that of the improved cultivars, while the polymorphism within populations for 12.5% of landraces was similar with that of the improved cultivars. For 81.2% of rice landraces， the number of alleles (Na) and Nei’s genetic diversity index (He), were higher than those of the improved cultivars, and for 18.8% of landraces, the figures were same as or slightly smaller than those of the improved cultivars. The He within populations of rice landraces significantly differed, ranging from 0.0146 to 0.5117, while Huangbansuo1(Huangbansuo collected in 1980), Huangbansuo2(Huangbansuo collected in 2007), Maxiangu1(Maxiangu collected in 1980) and Maxiangu2(Maxiangu collected in 2007) were the highest with figures of 0.2327, 0.4214, 0.5117 and 0.4489, respectively. Heterozygosity within rice varieties for 87.5% of rice landraces was significantly higher than that of the improved cultivars. AMOVA showed that 1/4 genetic difference among rice landraces were from within populations of rice landraces (P<0.001). The polymorphism, Ne, He and percentage of variation within populations of rice landraces at RM333, RM257 and RM180 were higher than others. It was speculated that RM333, RM257 and RM180 were suitable for testing genetic diversity within rice landraces of Yunnan Province.

Key words： rice; landrace; genetic diversity within population; microsatellite marker

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