水稻矮秆突变体Zj88d的鉴定与基因定位

纪现军1,2 ,叶胜海2 ,周涯2,3 ,修芬连2,3,邓晓梅1,2,尚海漩2,3,刘继云2,3,陈萍萍2,3,李小华1,2,金庆生2,张小明2,*

doi:10.3969/j.issn.10017216.2013.01.005

中国水稻科学 >

2013 , Vol. 27 >Issue 1: 35 - 40

DOI: https://doi.org/10.3969/j.issn.10017216.2013.01.005

研究报告

水稻矮秆突变体Zj88d的鉴定与基因定位

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¹杭州师范大学生命与环境科学学院，浙江杭州 310036； ²浙江省农业科学院作物与核技术利用研究所，浙江杭州 310021；³浙江师范大学化学与生命科学学院，浙江金华 321004；

收稿日期: 2012-03-13

修回日期: 2012-08-22

网络出版日期: 2013-01-10

基金资助

国家自然科学基金资助项目（31071207， 31100142）；浙江省科技项目（2007C12902， 2010R50033）；公益性行业（农业）科研专项（201103007）。

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Characterization and Gene Mapping of a Dwarf Mutant Zj88d in Rice

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¹ College of Life and Environment Sciences, Hangzhou Normal University, Hangzhou 310036, China; ² Institute of Crop and Nuclear Technology Utilization， Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China; ³ College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 321004, China;

Received date: 2012-03-13

Revised date: 2012-08-22

Online published: 2013-01-10

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摘要

用甲基磺酸乙酯诱变处理常规粳稻浙粳88，获得了矮秆水稻突变体Zj88d，多代自交稳定遗传。遗传分析表明突变体Zj88d的矮秆性状受一对隐性核基因控制。采用图位克隆方法，将目的基因定位在水稻第7染色体短臂末端744w和RM5344间的603 kb区间。排查该区间包含的100个候选基因，发现已克隆的矮秆基因OSH15也位于其中。测序结果显示，突变体Zj88d中的OSH15基因在第2内含子最末位发生“g→a”单碱基突变。

关键词： 水稻; 矮秆; 等位基因; 单碱基突变; 选择性剪切

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纪现军1,2 ,叶胜海2 ,周涯2,3 ,修芬连2,3,邓晓梅1,2,尚海漩2,3,刘继云2,3,陈萍萍2,3,李小华1,2,金庆生2,张小明2,* . 水稻矮秆突变体Zj88d的鉴定与基因定位[J]. 中国水稻科学, 2013 , 27(1) : 35 -40 . DOI: 10.3969/j.issn.10017216.2013.01.005

Abstract

The stably inherited dwarf mutant Zj88d was produced by treating the dry seeds of japonica rice variety Zhejing 88 with ethyl methylsulfonate (EMS). Genetic analysis of the dwarf mutant Zj88d revealed that this trait is controlled by one pair of recessive nuclear gene. The gene was located in the interval of 603 kb between 744w and RM5344 markers on the short arm of chromosome 7 by mapbased cloning. The dwarf gene “OSH15” was identified by screening one hundred candidate genes on the target chromosomal segment. Sequencing results further revealed that Zj88d gene has a singlebase substitution (g to a) in the last nucleotide of intron 2 of OSH15 allele.

Key words： rice; dwarf; allele; singlebase substitution; alternative splicing

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