利用RNA干涉研究水稻锌指蛋白基因OsZRL的功能

中国水稻科学 >

2013 , Vol. 27 >Issue 1: 9 - 16

DOI: https://doi.org/10.3969/j.issn.10017216.2013.01.002

研究报告

利用RNA干涉研究水稻锌指蛋白基因OsZRL的功能

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¹重庆大学生命科学学院，重庆 400044; ²合肥工业大学生物与食品工程学院，安徽合肥 230009；

收稿日期: 2012-03-21

修回日期: 2012-05-02

网络出版日期: 2013-01-10

基金资助

国家自然科学基金资助项目(30971752, 30970260)；重庆市科技攻关计划资助项目(2010AA1019)。

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Function Analysis of a Rice Zinc Finger Protein Gene OsZRL by RNA Interference

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¹ College of Life Sciences, Chongqing University, Chongqing 400044, China; ² School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China;

Received date: 2012-03-21

Revised date: 2012-05-02

Online published: 2013-01-10

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摘要

通过RNA干涉对一个水稻锌指蛋白基因OsZRL的功能进行分析。半定量RTPCR、定量PCR分析结果显示，转基因植株OsZRL基因表达水平显著下调。与野生型相比，OsZRL表达水平下调的转基因株系叶片变大，根系、茎秆更为发达，表明OsZRL的下调对水稻植株的生长有促进作用。OsZRL基因的表达模式和转基因幼苗表型显示OsZRL参与赤霉素、脱落酸信号途径。因而推测锌指蛋白OsZRL是受赤霉素、脱落酸调节的水稻生长发育负调控因子。

关键词： 水稻锌指蛋白; RNA干涉; 负调控; 赤霉素; 脱落酸

本文引用格式

玉晓红1 ,晁丽1,陈昌冬1 ,刘永胜1，2 ,牛向丽1, 2, * . 利用RNA干涉研究水稻锌指蛋白基因OsZRL的功能[J]. 中国水稻科学, 2013 , 27(1) : 9 -16 . DOI: 10.3969/j.issn.10017216.2013.01.002

Abstract

The function of a rice zincfinger protein gene (OsZRL) was investigated by RNA interference. The results of semiquantitative RTPCR and quantitative PCR analysis indicated that OsZRL expression levels were significantly downregulated in the transgenic plants. The OsZRLRNAi transgenic lines were characterized by bigger leaves, stronger roots and stems compared to wild type plants, indicating that the suppression of OsZRL had a positive effect on rice growth. The expression pattern and transgenic seedlings phenotypes revealed that OsZRL was involved in gibberellin or abscisic acid signaling pathway. It suggests that zincfinger protein OsZRL might perform its function of negetive regulator in rice growth through the regulation of gibberellin and abscisic acid.

Key words： rice zincfinger protein; RNA interference; negative regulation; gibberellin; abscisic acid

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