籼稻矮化多分蘖突变体gsor23的基因克隆与表达分析

中国水稻科学 >

2013 , Vol. 27 >Issue 1: 1 - 8

DOI: https://doi.org/10.3969/j.issn.10017216.2013.01.001

研究报告

籼稻矮化多分蘖突变体gsor23的基因克隆与表达分析

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¹ 中国农业科学院作物科学研究所/农作物基因资源与基因改良国家重大科学工程，北京 100081；² 山东省水稻研究所, 山东济南 250100；³ 南京农业大学作物遗传与种质创新国家重点实验室/江苏省植物基因工程技术研究中心, 江苏南京210095；

收稿日期: 2012-03-01

修回日期: 2012-04-18

网络出版日期: 2013-01-10

基金资助

国家转基因生物新品种培育科技重大专项(2011ZX08009003)；留学人员科技活动择优资助项目。

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Positional Cloning and Expression Analysis of the Gene Responsible for the Hightillering Dwarf Phenotype in the indica Rice Mutant gsor23

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¹ National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agriculture Sciences, Beijing 100081, China; ² Shandong Rice Research Institute, Jinan 250100, China; ³ National Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing 210095, China;

Received date: 2012-03-01

Revised date: 2012-04-18

Online published: 2013-01-10

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摘要

矮化多分蘖突变体gsor23是籼稻品种Indica9经γ射线辐射后获得的。遗传分析表明其矮化多分蘖表型受一个隐性基因控制，并将突变基因定位在第1染色体长臂上InDel标记C1WT2与C1WT4之间。这两个标记之间物理距离为386 kb，其间包含一个抑制植物分枝的基因D10。对gsor23中的D10基因测序发现编码区第404位的碱基C缺失，导致从第135位氨基酸开始移码突变。gsor23的突变位点与已报道的粳稻背景的d101和d102突变体不一样，是D10基因一个新的等位突变体。D10编码的类胡萝卜素裂解双加氧酶8 (carotenoidcleaving dioxygenase, CCD8)，是抑制分枝的新型植物激素独脚金内酯(strigolactones, SLs)合成途径中的关键酶之一。用SLs人工合成类似物GR24处理gsor23，其多分蘖表型受到抑制。实时 RTPCR结果显示D10在水稻根部表达量较高，叶片较低。在突变体gsor23中，参与 SLs合成的基因D10上调表达，而可能参与SLs信号转导的基因D3和D14表达下调。

关键词： 分枝; 独脚金内酯; 类胡萝卜素裂解双加氧酶; 反馈调控

本文引用格式

王涛1,袁守江2 ,尹亮2 ,赵金凤1,万建民1,3,李学勇1,* . 籼稻矮化多分蘖突变体gsor23的基因克隆与表达分析[J]. 中国水稻科学, 2013 , 27(1) : 1 -8 . DOI: 10.3969/j.issn.10017216.2013.01.001

Abstract

Hightillering dwarf mutant gsor23 was generated from the indica rice variety Indica9 by γray radiation. Genetic analysis showed that this phenotype was controlled by one single recessive gene, which was mapped within a physical distance of 386 kb between two InDel markers C1WT2 and C1WT4 on the long arm of chromosome 1. There is a known gene D10 within this region and mutations in which caused hightillering phenotype in rice. Sequence analysis of the D10 allele in gsor23 revealed that the base C at the 404th position in the coding region was deleted, causing frameshift mutation after the 134th amino acids. This mutant differed from the previously reported japonica mutants d101 and d102 in mutation site and genetic background.Therefore, gsor23 is a novel allellic mutant of D10, which encodes the carotenoidcleaving dioxygenase 8 (CCD8), a key enzyme involved in the biosynthesis of the new plant hormone strigolactones(SLs). After treatment with GR24, a synthetic analogues of SLs, gsor23 showed normal phenotype. Realtime RTPCR analysis showed that D10 expression level was high in root, but low in leaves. Compared with the wildtype Indica9, the expression level of the SLs biosynthetic gene D10 was upregulated, while genes likely involved in the SLs signal transduction such as D3 and D14 were downregulated in the gsor23 mutant.

Key words： branching; strigolactones; carotenoidcleaving dioxygenase; feedback regulation

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