在实验室条件下，以感虫品种TN1、抗虫品种IR36和抗旱品种旱优3号为材料，采用聚乙二醇6000（PEG6000）模拟干旱处理水稻植株，研究干旱胁迫对褐飞虱取食和产卵的影响。结果表明，在同一水稻品种上，褐飞虱雌成虫在稻株上数量随着PEG6000浓度的上升而显著减少（P<0.001），而褐飞虱若虫在不同PEG6000浓度处理的水稻植株上数量无显著差异（P=0.093）。褐飞虱雌成虫在48 h内的蜜露分泌量和在稻株上72 h内的产卵量均与PEG6000浓度呈显著负相关。在感虫品种TN1上，褐飞虱若虫在不同浓度PEG6000处理稻株上的分布无显著差异；而在抗旱品种旱优3号和抗虫品种IR36上，随着PEG6000浓度的上升，水稻基部的褐飞虱若虫数量明显增多。在TN1和旱优3号上，褐飞虱雌成虫蜜露分泌量随PEG6000浓度上升而显著减少；而在IR36上，褐飞虱雌成虫在不同浓度PEG6000处理水稻植株上的蜜露分泌量均很少且无显著差异。
The feeding behavior and oviposition preference of brown planthoppers (BPH), Nilaparvata lugens Stl, on rice varieties TN1 (susceptible), IR36 (with resistant gene bph2), Hanyou 3 (droughttolerant) were determined under drought stress simulated with polyethylene glycol (PEG6000) in laboratory. In the choice test, the percentage of BPH female adult on rice plants under different concentrations of PEG6000 decreased significantly with the rising PEG6000 concentration (P<0.001).However, no marked difference in the percentage of BPH nymph was found (P=0.093). The number of BPH eggs per plant within 72 h was negatively correlated with the concentration of PEG6000 on TN1 and IR36 (P=0.001 and P=0.008, respectively). On the rice plants of IR36 and Hanyou 3, BPH nymph tended to move from uppermiddle to basal part of leaf sheath when the PEG6000 concentration increased. However, it didn’t happen on TN1. The amount of honeydew per female adult within 48h reduced on TN1 and Hanyou 3 with the increasing concentration of PEG6000, while on IR36 it was very low and no significant difference was found under different concentrations of PEG6000.
\[1\]程家安, 朱金良, 祝增荣, 等. 稻田飞虱灾变与环境调控. 环境昆虫学报, 2008, 30(2): 176182.
\[2\]Dale G B, Kenneth G S. Resurrecting the ghost of green revolutions past: The brown planthopper as a recurring threat to highyielding rice production in tropical Asia.J AsiaPacif Entomol, 2012, 15: 122140.
\[3\]曹明奎, 陶波, 李克让. 全球气候变化背景下中国降水量空间格局的变化特征. 地理研究, 2006, 25(6): 11311140.
\[4\]IPCC. Climate Change 2001: Impacts, Adaptation, and Vulnerability. Cambridge: Cambridge University Press, 2001:1181.
\[5\]Bale J S, Masters G J, Hodkinson I D, et al. Herbivory in global climate change research: Direct effects of rising temperature on insect herbivores. Glob Chan Biol, 2002, 8: 116.
\[6\]Pritchard J, Griffiths B, Hunt E J. Can the plantmediated impacts on aphids of elevated CO2 and drought be predicted? Glob Chan Biol, 2007, 13: 16161629.
\[7\]Mattson W J, Haack R A. The role of drought in outbreaks of planteating insect.BioScience, 1987, 27(2): 110119.
\[8\]Huberty A F, Denno R F. Plant water stress and its consequences for herbivorous insects: A new synthesis. Ecology, 2004, 85(5): 13831398.
\[9\]Baqui M A, Kershaw W J S. Effect of plant water stress on honeydew production, weight gain and oviposition of brown planthopper Nilaparvata lugens on rice cultivar. Entomol Exp Appl, 1993, 67: 2530.
\[10\]Simpson L S, Jackson G E, Grace J.The response of aphids to plant water stressthe case of Myzus persicae and Brassica oleracea var. capitata. Entomol Exp Appl, 2012, 142(3): 191202.
\[11\]刘宇锋, 高国庆, 李道远. 水稻抗旱生理生化及其相关基因研究进展. 中国农学通报, 2008, 22(2): 219224.
\[12\]肖景华, 罗利军. 水稻分子育种与绿色超级稻. 分子植物育种, 2010, 8(6): 10541058.
\[13\]陈贵, 周毅, 郭世伟, 等. 水分胁迫条件下不同形态氮素营养对水稻叶片光合效率的调控机理研究. 中国农业科学, 2007, 40(10): 21622168.
\[14\]戴高兴, 彭克勤, 萧浪涛, 等. 聚乙二醇模拟干旱对耐低钾水稻幼苗丙二醛、脯氨酸含量和超氧化物歧化酶活性的影响. 中国水稻科学, 2006, 20(5): 557559.
\[15\]Caruso A, Chefdor F, Carpin S, et al. Physiological characterization and identification of genes differentially expressed in response to drought induced by PEG 6000 in Populus canadensis leaves. J Plant Physiol, 2008, 165(9): 932941.
\[16\]Behle R W, Michels G J. Responses of greenbug to drought stressed small grain hosts.Southwest Entomol, 1988, 13(1): 5562.
\[17\]Summer L C, Need J T, McNew R W, et al. Response of Schizaphis graminum (Homoptera: Aphididae) to drought stressed wheat using polyethylene glycol as a matricum. Environ Entomol, 1983, 12(3): 919922.
\[18\]Michel B E, Kaulmann M. The osmotic potential of polyethylene glycol 6000. Plant Physiol, 1973, 51: 914916.
\[19\]White T C R. The abundance of invertebrate herbivores in relation to the availability of nitrogen in stressed food plants. Oecologia, 1984, 63(1): 90105.
\[20\]EnglishLoeb G M, Stout M J, Duffey S S. Drought stress in tomatoes: Changes in plant chemistry and potential nonlinear consequences for insect herbivores. Oikos, 1997, 79: 456468.
\[21\]Steven S L, Colin M O. Soil nutrients and water availability interact to influence willow growth and chemistry but not leaf beetle performance. Entomol Exp Appl, 2003, 107(1): 6979.
\[22\]Gutbrodt B, Mody K, Dorn S. Drought changes plant chemistry and causes contrasting responses in lepidoteran herbivores. Oikos, 2011, 120(11): 17321740.
\[23\]Louda S M, Collinge S K. Plant resistance to insect herbivores: A field test of the environmental stress hypothesis. Ecology, 1992, 73: 153169.
\[24\]Thomas A T, Hodkinson I D. Nitrogen, water stress, and the feeding efficiency of lepidopteran herbivores. J Appl Ecol, 1991, 28(2): 703720.
\[25\]Hale B K, Bale J S, Pritchard J,et al. Effects of host plant drought stress on the performance of the bird cherryoat aphid, Rhopalosiphum padi (L.): A mechanistic analysis. Ecol Entomol, 2003, 28(6): 666677.
\[26\]Isaacs R, Byrne D N, Hendrix D L. Feeding rates and carbohydrate metabolism by Bemisia tabaci (Homoptera: Aleyrodidae) on different quality phloem saps. Physiol Entomol, 1998, 23(3): 241248.
\[27\]方锡涛. 水势与土壤植物大气连续体系中的水分运动. 浙江农业大学学报, 1993, 19(2): 231238.
\[28\]马廷臣, 余蓉蓉, 陈荣军, 等. PEG6000模拟干旱对水稻苗期根系形态和部分生理指标影响的研究. 中国农学通报, 2010, 26(8): 149156.
\[29\]朱维琴, 吴良欢, 陶勤南. 干旱逆境对不同品种水稻生长、渗透调节物质含量及保护酶活性的影响.科技通报, 2006, 22(2): 176181.
\[30\]Kiritani K. Predicting impacts of global warming on population dynamics and distribution of arthropods in Japan. Popul Ecol, 2006, 48(1): 512.
