采用40对SSILP、30对InDel和43对SSR标记对来自斯里兰卡的28份杂草稻和14栽培稻,来自国内外的9份籼型栽培稻、7份粳型栽培稻和4份代表性杂草稻进行遗传多样性分析。SSILP、InDel和SSR标记分别检测到84、61和213个等位基因,其平均多态性信息含量(PIC)分别为0.257、0.255和0.543。聚类分析显示供试的42份斯里兰卡杂草稻和栽培稻均为籼型。SSILP与InDel标记的相关性很高(r=0.989),而它们与SSR标记相关性较低(0.812和0.808)。结果表明,SSILP和InDel标记可高效鉴别各类稻种资源的籼粳属性,而SSR标记更适用于亚种内的分类。
Forty SSILP, 30 InDel and 43 SSR markers were used to compare genetic diversity of 42 weedy and cultivated rice entries from Sri Lanka, 9 indica and 7 japonica rice cultivars as well as four representatives of weedy rice from China and other countries(regions). There were 84, 61 and 213 alleles identified by SSILP, InDel and SSR markers, with average polymorphism information content (PIC) values 0.257, 0.255 and 0.543, respectively. The NJ clusters analysis indicated all 42 Sri Lanka weedy and cultivated rice entries belonged to indica type. The genetic distances of SSILP and InDel markers revealed highly correlation (r=0.989). Meanwhile the associations between SSR markers and the other two markers (SSILP and InDel) were lower as 0.812 and 0.808, respectively. With the rising reliability and accuracy of indicajaponica classification resulted from the development of more subspeciesspecific markers, SSILP and InDel markers can be applied extensively for the indicajaponica classification of rice germplasm. And SSR markers can classify better in the subspecies.
\[1\]Melchinger A E, Messmer M M, Lee M, et al. Diversity and relationships among UnitedStates maize inbreds revealed by restriction fragment length polymorphisms. Crop Sci, 1991, 31: 669678.
\[2\]Wei X H, Yuan X P, Yu H Y, et al. Temporal changes in SSR allelic diversity of major rice cultivars in China. J Genet Genom, 2009, 36, 363370.
\[3\]于萍, 袁筱萍, 徐群, 等. 中国常规稻主栽品种的遗传结构及籼粳组分变化.中国水稻科学, 2011, 25(4): 387391.
\[4\]Zhang H L, Sun J L, Wang M X, et al. Genetic structure and phylogeography of rice landraces in Yunnan, China, revealed by SSR. Genome, 2007, 50: 7283.
\[5\]张晓丽,郭辉,王海岗,等. 中国普通野生稻与栽培稻种SSR多样性的比较分析. 作物学报,2008, 34(4): 591597.
\[6\]赵伟,夏寒冰,章淑杰,等. 籼粳特异插入/缺失分子标记揭示的稻属植物遗传分化. 复旦学报: 自然科学版,2008,47(3): 281287.
\[7\]Wang X S, Zhao X Q, Zhu J, et al. Genomewide investigation of intron length polymorphisms and their potential as molecular markers in rice (Oryza sativa L.). DNA Res, 2005, 12: 417427.
\[8\] Zhao X Q, Yang L, Zheng Y, et al. Subspeciesspecific intron length polymorphism markers reveal clear genetic differentiation in common wild rice (Oryza rufipogon L.) in relation to the domestication of cultivated rice (O. sativa L.). J Genet Genom, 2009, 36: 435442.
\[9\]Suh H S. Weedy rice. Wild Crop Germplasm Center & Yeungnam University, Korea, 2008: 1317.
\[10\]王黎明,陈勇. 杂草稻研究现状及利用展望. 植物保护, 2009, 35(5): 1417.
\[11\]Suh H S, Sato Y I, Morishima H. Genetic characterization of weedy rice (Oryza sativa L.) based on morphophysiology, isozymes and RAPD markers. Theor Appl Genet, 1997, 94:316321.
\[12\]Chen L J, Suh H S, Lee D S. Evolutionary significance of Chinese weedy rice “Lutao”. SABRAO J Breed Genet, 2001, 33(2): 99109.
\[13\]Panaud O, Chen X L, McCouch S R. Development of microsatellite markers and characterization of simple sequence length polymorphism (SSLP) in rice (Oryza sativa L.). Mol Gen Genet, 1996, 252: 597607.
\[14\]Liu K, Muse S V. Power Marker: An integrated analysis environment for genetic marker analysis. Bioinformatics, 2005, 21: 21282129.
\[15\]Patzak J. Comparison of RAPD, STS, ISSR and AFLP molecular methods used for assessment of genetic diversity in hop (Humulus lupulus L.). Euphytica, 2001, 121: 918.
\[16\]Peakall R, Smouse P E. GENALEX 6: Genetic analysis in Excel. Population genetic software for teaching and research. Mol Ecol Notes, 2006, 6(1): 288295.
\[17\]Excoffier L, Laval L G, Schneider S. Arlequin (version 3.0): An integrated software package for population genetics data analysis. Evol Bioinform Online, 2005, 1: 4750.
\[18\]Yu G Q, B Y, Shi C H, Dong C Q, et al. Genetic diversity and population differentiation of Liaoning weedy rice detected by RAPD and SSR markers. Biochem Genet, 2005, 43: 261270.
\[19\]Zhang L J, Dai W M, Wu C, et al. Genetic diversity and origin of japonica and indicalike rice biotypes of weedy rice in the Guangdong and Liaoning provinces of China. Genet Resour Crop Evol, 2012,59:399410.
\[20\]Cho Y G, Blair M W, Panaud O, et al. Cloning and mapping of variety specific rice genomic DNA sequences amplified length fragment polymorphisms (AFLP) from silverstained polyacrylamide gels. Genome, 1995, 39: 373378.
\[21\]Bres P C, Bangratz M, Ghesquiere A. Genetic diversity and population dynamics of weedy rice in the Camargue. Genet Select, 2001, 33(1): 425440.
\[22\]Garcia A A F, Benchimol L L, Barbosa A M M, et al. Comparison of RAPD, RFLP, AFLP and SSR markers for diversity studies in tropical maize inbred lines. Genet Mol Biol, 2004, 27: 579588.
\[23\]Heckenberger M, van der Voort J R, Melchinger A E, et al. Variation of DNA fingerprints among accessions within maize inbred lines and implications for identification of essentially derived varieties: Ⅱ. Genetic and technical sources of variation in AFLP data and comparison with SSR data. Mol Breeding, 2003, 12: 97106.
\[24\]Huang M, Xie F M, Chen L Y, et al. Comparative analysis of genetic diversity and structure in rice using ILP and SSR markers. Rice Sci, 2010, 17(4):257268.
\[25\]Xu X M, Liang K J, Zhang S G, et al. Analysis of indicajaponica differentiation in rice parents and derived lines using ILP markers. Agric Sci China, 2009, 8(12): 14091418.