水稻条斑和颖花异常突变体stfon的鉴定与遗传分析

陈德西1，2 ,李婷2 ,曲广林2,黄文娟2 ,何忠全1,李仕贵2，*

doi:10.3969/j.issn.10017216.2012.06.007

中国水稻科学 >

2012 , Vol. 26 >Issue 6: 677 - 685

DOI: https://doi.org/10.3969/j.issn.10017216.2012.06.007

研究报告

水稻条斑和颖花异常突变体stfon的鉴定与遗传分析

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¹四川省农业科学院植物保护研究所, 四川成都 610066; ²四川农业大学水稻研究所，四川温江 611130；

收稿日期: 2011-12-27

修回日期: 2012-02-06

网络出版日期: 2012-11-10

基金资助

教育部创新团队发展计划资助项目（IRT0453）；四川省财政基因工程优秀论文基金资助项目 (2011LWJJ005)。

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Characterization and Genetic Analysis of a Streaked and Abnormal Glumous Flower Mutant stfon

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¹ Institute of Plant Protection, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China； ² Rice Research Institute, Sichuan Agricultural University, Chengdu 611130, China;

Received date: 2011-12-27

Revised date: 2012-02-06

Online published: 2012-11-10

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摘要

在水稻遗传转化过程中发现一个不含外源基因的条斑和颖花异常的双突变体，暂命名为stfon。该突变体除茎、叶、穗出现白条斑外，花器数目增多，颖花开裂。其极端表型为同一颖花中长出几朵小花或小花中枝梗伸长为花序。利用透射电镜对苗期叶片白色组织细胞超微结构进行观察，发现质体结构异常，不能发育成正常叶绿体所具有的片层和类囊体，叶绿体的发育阻断在质体发育早期。利用扫描电镜和石蜡切片对花器官形态发生过程进行观察，发现花分生组织生长具有无序和无限性。对扭曲的叶片石蜡切片观察，发现维管束鞘细胞过度生长，或一些泡状细胞增大。将突变体与4个叶色正常的材料进行杂交和回交，遗传分析表明突变性状呈细胞质遗传。

关键词： 水稻; 条斑; 花器官; 解剖结构; 胞质遗传

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陈德西1，2 ,李婷2 ,曲广林2,黄文娟2 ,何忠全1,李仕贵2，* . 水稻条斑和颖花异常突变体stfon的鉴定与遗传分析[J]. 中国水稻科学, 2012 , 26(6) : 677 -685 . DOI: 10.3969/j.issn.10017216.2012.06.007

Abstract

A double mutant with striped leaf and abnormal glumous flower containing no foreign genes, was found and temporarily termed as stfon. Besides white streak on stems, leaves and panicles,it was also characterized by increased number of floral organs and cracked glumous flowers. In extreme cases,the glumous flower was composed of several florets or its middle branch rachis on florets extended,like a looking inflorescence. Through observing the ultra microstructures of white histocytes at the seedling stage with a scanning electron microscope, the white tissue showed abnormal plastid structures, lamellas and thylakoids which cannot be developed into normal chloroplast. Moreover, the development of chloroplast is stopped at the early stage of plastid growth. Scanning electron microscope observation and paraffin sections of floral organs revealed that the floral meristem organ development was out of order and unlimited, while in the twisty leaves, vascular cells growed excessively, or some bubbly cells increased. Genetic analyses indicated that the mutant character followed cytoplasm inheritance.

Key words： rice; streak; floral organ; anatomical structure; plasmatic inheritance

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