从水稻基因组DNA中扩增出1 184 bp的Ospgip1基因片段。该片段包含930 bp的完整开放阅读框，终止密码子为TAA，无内含子。RTPCR结果表明，Ospgip1基因在水稻抗、感纹枯病品种中均能表达，但不同生育期、不同部位表达量有差异。实时PCR结果显示，抗病品种YSBR1和中感品种Jasmine 85中Ospgip1的表达量要明显高于感病品种Lemont；稻苗黄化处理后，无论是抗病品种还是感病品种的Ospgip1表达量均显著提高；纹枯病菌侵染使得抗病品种Ospgip1表达量大大增加，而对感病品种影响不大。
陈夕军1, 2,刘晓维1,左示敏2,童蕴慧1,潘学彪2,*,徐敬友1,*
. 水稻抗感纹枯病品种Ospgip1基因的表达特征[J]. 中国水稻科学, 2012
, 26(5)
: 629
-632
.
DOI: 10.3969/j.issn.10017216.2012.05.018
A fragment of 1 184 bp of the Ospgip1 gene including a complete open reading frame (OFR) of 930 bp had been amplified, which had the termination codon of TAA and no intron. RTPCR results showed that the Ospgip1 gene could be expressed in all the rice cultivars while their expression levels differed among growth stages or organs. Realtime PCR results indicated that the expression levels in resistant cultivars YSBR1 and Jasmine 85 were significantly higher than that in the susceptible cultivar Lemont. The expression level was also upregulated in the etiolated seedlings. Inoculation with the pathogen of rice sheath blight significantly upregulated the expression level of the Ospgip1 gene in resistant cultivars while exerted no impact on the expression level of the gene in the susceptible one.
\[1\]Protsenko M A, Buza N L, Krinitsyna A A, et al. Polygalacturonaseinhibiting protein is a structural component of plant cell wall. Biochemistry, 2008, 73(10): 10531062.
\[2\]Spinelli F, Mariotti L, Mattei B, et al. Three aspartic acid residues of polygalacturonase inhibiting protein (PGIP) from Phaseolus vulgaris are critical for inhibition of Fusarium phyllophilum PG. Plant Biol, 2009, 11(5): 738743.
\[3\]Dwayne D H, Li R G, Lone B, et al.Brassica napus possesses an expanded set of polygalacturonase inhibitor protein genes that are differentially regulated in response to Sclerotinia sclerotiorum infection, wounding and defense hormone treatment. Planta, 2008, 228: 241253.
\[4\]Cervone F, Hahn M G, De Lorenzo G, et al. Hostpathogen interactions: XXXIII. A plant protein converts a fungal pathogenesis factor into an elicitor of plant defense responses. Plant Physiol, 1989, 90: 542548.
\[5\]Bergmann C, Ito Y, Singer D, et al.Polygalacturonaseinhibiting protein accumulates in Phaseolus vulgaris L. in response to wounding, elicitors and fungal infection. Plant J, 1994, 5:625634.
\[6\]De Lorenzo G, D′Ovidio R, Cervone F. The role of polygacturonaseinhibiting proteins (PGIPs) in defense against pathogenic fungi. Annu Rev Phytopathol, 2001, 39: 313335.
\[7\]Federici L, Di Matteo A, FernandezRecio J, et al.Polygalacturonases inhibiting proteins: Players in plant innate immunity? Trends Plant Sci, 2006, 11(2): 6570.
\[8\]Cervone F, De Lorenzo G, Pressey R. Can Phaseolus PGIP inhibit pectic enzymes from microbes and plants? Phytochemistry, 1990, 29: 447449.
\[9\]Johnston D J, Ramanathan V, Williamson B, et al. A protein from immature raspberry fruits which inhibits endopolygalacturonases from Botrytis cinerea and other microorganisms. Exp Bot, 1993, 44(5): 971976.
\[10\] 阮期平, 周立, 郑远旗, 等. PGIP在植物抗病方面的研究进展. 植物学通报, 2000, 17(1): 6063.
\[11\]阮期平, 周立, 李建吾, 等. 小麦品种的抗赤霉病性与PGIP含量和分布的关系. 植物保护学报, 2002, 29(2): 101104.
\[12\] Chung C T, Niemela S L, Miller R H. Onestep preparation of competent Escherichia coli: Transformation and storage of bacterial cells in the same solution. Proc Natl Acad Sci USA, 1989, 86(8): 21722175.
\[13\]廖皓年, 肖陵生, 王华生. 水稻纹枯病发生历史及演变原因简析. 广西植保, 1997(3): 3538.
\[14\]左示敏, 张亚芳, 殷跃军, 等. 田间水稻纹枯病抗性鉴定体系的确立与完善. 扬州大学学报: 农业与生命科学版, 2006, 27(4): 5761.
\[15\]张红, 陈夕军, 童蕴慧, 等.纹枯病菌胞壁降解酶对水稻组织和细胞的破坏作用. 扬州大学学报: 农业与生命科学版, 2005, 26(4): 8386.
\[16\] Ferrari S, Vairo D, Ausubel F M, et al. Tandemly duplicated Arabidopsis genes that encode polygalacturonaseinhibiting proteins are regulated coordinately by different signal transduction pathways in response to fungal infection. Plant Cell, 2003, 15: 93106.
\[17\]Favaron F, Castiglioni C, Lenna D. Inhibition of some rot fungi polygalacturonases by Allium cepa L and Allium porrum L extracts. J Phytopathol, 1993, 139: 201206.
\[18\]陈夕军, 刘晓维, 左示敏, 等. 水稻多聚半乳糖醛酸酶抑制蛋白基因(Ospgip1)原核表达及编码产物生物信息学分析. 中国水稻科学, 2011, 25(2): 136142.
\[19\] Machinandiarena M F, Olivieri F P, Daleo G R, et al.Isolation and characterization of a polygalacturonase inhibiting protein from potato leaves. Accumulation in response to salicylic acid, wounding and infection. Plant Physiol Biochem, 2001, 39: 129136.
\[20\]陈夕军, 王玲, 左示敏, 等. 水稻纹枯病寄主病原物互作鉴别品种与菌株的筛选. 植物病理学报, 2009, 39(5): 514520.
