The kinds of cell wall degrading enzymes (CWDEs) from Rhizoctonia solani and their pathogenic effect were analyzed by the SDSpolyacrylamide gel electrophoresis (SDSPAGE), enzyme activity measurement, determination of leaky reducing sugar and relative conductivity of leachate from leaf tissues treated with CWDEs. The results of SDSPAGE of CWDEs revealed that there were at least 10 clear bands of proteins, indicating that there were at least 10 kinds of components with different molecular weight in the mixture of CWDEs. The enzyme activities of seven kinds of common CWDEs’ were determined, and the results showed that the activity of polygalacturonase (PG) was the highest, followed by polymethylgalacturonase (PMG). Through comparing the effects of different substrates on the induction of three enzymes, it was found that 1.0% sodium carboxymethyl cellulose (CMC) was the most suitable substrate for the induction of endoβ1,4glucanase (Cx), whereas 1.0% pectin for the induction of PG and PMG. The best enzyme reaction conditions for the three most important CWDEs, i.e. PG, Cx and PMG, were explored from three aspects including temperature, reaction time and pH value. In regarding to reaction temperature, it was found that the best reaction temperature was 30℃ for Cx, and 50℃ for PG and PMG. In regarding to reaction time, it was found that 40 min was more suitable for Cx and PMG, whereas 60 min was more suitable for PG. In regarding to pH value, the results indicated that the enzyme activities of PG, Cx and PMG were the highest when the pH values reached 5.0, 9.0 and 4.0 to 6.0, respectively. Through the determination of leaky reducing sugar and relative conductivity of leachate from leaf tissues treated with CWDEs, it was obvious that CWDEs did cause damage to rice leaf tissues and the damage degrees increased with the increasing concentrations of CWDEs.
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