为了探索影响高原粳稻区白叶枯病菌变异的因子，以2个云南高原粳稻区白叶枯菌株20012、K1为供试菌株，在毫糯扬、金南风、滇粳优5号和合系41等不同品种组合上连续接种或连续交叉接种后分离菌株，利用高原粳稻白叶枯病菌的鉴别品种和分子检测手段，对获得的菌株的致病型及基因变异进行分析。结果显示，原始菌株20012与其7个获得菌系（23个菌株）的致病型都不同，其中，除获得菌系62的3个菌株致病力增强外，其他6个获得菌系的20个菌株致病力均减弱；而原始菌株K1的抗感反应模式与其获得菌系68的7个菌株完全相同。利用IS1112、hrpF引物进行菌株PCR扩增以及UPGMA聚类分析并将相应序列测序，结果显示，供试菌株IS1112、hrpF分子谱型的遗传分簇与菌株致病型之间无一一对应关系；在基因序列上，各菌株的IS1112和hrpF的两个序列片段表现出不同的碱基变化特征。两个原始菌株接种在不同抗性品种组合上获得的菌系的致病性变异具有特殊性和不定向性。
杨雅云1,3,4,# ,彭新禧1,2,#,徐福荣1,3,4,和建平1,2,阿新祥1,3,4,董超1,3,4,张恩来1,3,4,张斐斐1,3,4 ,汤翠凤1,3,4,戴陆园1,3,4,*
. 连续交叉接种对云南高原粳稻白叶枯病菌株变异的影响[J]. 中国水稻科学, 2012
, 26(5)
: 583
-591
.
DOI: 10.3969/j.issn.10017216.2012.05.011
To study the factors influenceing variation of Xanthomonas oryzae pv. oryzae( Xoo) from japonica rice in Yunnan plateau, eight strains(30 isolates) derived from two original strains 20012 and K1 were obtained through successivecross inoculation.Their pathotype and gene variation were studied by using differential cultivars and molecular detection methods. The results showed that seven obtained strains including 23 isolates observably differed with the original isolate 20012 in pathotype, beside the pathogenicity of 62 containing 3 isolates were increased, while the pathogenicity of the other six strains containing 20 isolates were decreased. The pathotypes of 68 were the same as that of its original isolate K1. By using UPGMA analysis of PCR producets of primer IS1112 and hrpF, no relationship between molecular haplotypes and pathotypes were found. Sequence analysis showed that basic group variation in sequences of all strains amplified by primers IS1112 and hrpF differed. The pathogenic variation of obtained strains from two original strains inoculated on various varieties were unique and nondirective.
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