利用D50/HB277衍生的重组自交系群体，对水稻株高进行QTL分析。在杭州和海南两种环境条件下，共检测到6个控制株高的加性效应QTL，分别位于第1、2、3、4、6和8染色体上，其中,位于第3染色体的QTL qPH3效应较大，在两种环境条件均能稳定表达且贡献率分别为16.52%和16.60%。进一步筛选获得1个剩余杂合体，对其衍生的RHLF2群体进行遗传分析，其qPH3检测贡献率达76.13%。通过后代验证，最终将其锁定于标记RM16211和RM16237之间物理距离约204 kb区域内。这将为该基因的精细定位及克隆奠定基础。
A recombinant inbred population derived from the cross between a javonica（D50）and an indica（HB277）rice was used to investigate the genetic factors affecting plant height. Ａ total of six quantitative trait loci（QTLs）with significant additive effect were identified for the plant height, and they were located on chromosome 1, 2, 3, 4, 6 and 8. One QTL qPH3 can stably express and have significant additive effects in Hangzhou, Zhejiang Province with the explained variance of 16.52% and Linshui, Hainan Province with the explained variance of 16.60%. One residual heterozygous line(RHL) was selected from the RILs and a RHLF2 population was constructed. The major QTL qPH3 with the explained variance of 76.13% was detected with the RHLF2 population. By progeny tests, qPH3 was finally mapped within about 204 kb in the region RM16211-RM16237. These results will be helpful for fine mapping and mapbased cloning of this QTL.
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