利用两个直链淀粉含量相似的水稻品种9311和日本晴为亲本，采取单粒传法创建由190个家系组成的重组自交系群体，并构建了包含202个SSR、CAP和STS标记的遗传连锁图谱。采用复合区间作图法，在两个不同生态环境下(三亚、合肥)对糙米蛋白质含量和精米蛋白质含量的QTL进行了定位分析，共定位到9个QTL。其中，qBRPC1.3在两个环境中均被检测到，稳定性较高，其贡献率达9.4%；位于第1染色体的RM578-RM10596是多效性区间，同时控制糙米蛋白质含量和精米蛋白质含量，贡献率分别为6.4%和5.7%。
Based on single seed descend method a recombinant inbred lines population consisting of 190 lines was created by crossing two rice varieties with similar apparent amylose content, Nipponbare (japonica) and 9311 (indica) by QTLs for brown rice protein content(BRPC) and milled rice protein content (MRPC) with a genetic linkage map including 202 SSR, CAP and STS markers were identified. By composite interval mapping method in two environments sites (Sanya Hainan Province, China and Hefei Anhui Province, China), a total of nine QTLs were identified, indicating that rice protein content was controlled by multiple genes. qBRPC1.3 was detected at the two sites with high stability, explaining 9.4% of total variation. The interval RM578-RM10596 on chromosome 1, controlled BRPC and MRPC, explaining 6.4% and 5.7% of total variation, respectively.
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