中国水稻科学

中国水稻科学 >

2012 , Vol. 26 >Issue 3: 351 - 355

DOI: https://doi.org/10.3969/j.issn.10017216.2012.03.014

研究简报

两个环境下糙米和精米蛋白质含量的QTL分析

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  • 安徽省农业科学院 水稻研究所， 安徽 合肥  230031；

收稿日期: 2011-09-13

  修回日期: 2011-10-19

  网络出版日期: 2012-05-10

基金资助

国家自然科学基金资助项目（31071401）；科技部转基因重大专项（2008ZX08001002， 2009ZX08001013B）；安徽省优秀青年基金资助项目（10040606Y03）； 国家863计划资助项目（2012AA1011024）。

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Identification of QTL for Protein Content in Brown and Milled Rice in Two Enironments

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  • Rice Research Institute, Anhui Academy of Agricultural Sciences, Hefei 230031, China;

Received date: 2011-09-13

  Revised date: 2011-10-19

  Online published: 2012-05-10

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摘要

利用两个直链淀粉含量相似的水稻品种9311和日本晴为亲本，采取单粒传法创建由190个家系组成的重组自交系群体，并构建了包含202个SSR、CAP和STS标记的遗传连锁图谱。采用复合区间作图法，在两个不同生态环境下(三亚、合肥)对糙米蛋白质含量和精米蛋白质含量的QTL进行了定位分析，共定位到9个QTL。其中，qBRPC1.3在两个环境中均被检测到，稳定性较高，其贡献率达9.4%；位于第1染色体的RM578-RM10596是多效性区间，同时控制糙米蛋白质含量和精米蛋白质含量，贡献率分别为6.4%和5.7%。

关键词： 水稻; 蛋白质含量; 数量性状基因座

本文引用格式

杨亚春,倪大虎,宋丰顺,李莉,冯光,李泽福,杨剑波* . 两个环境下糙米和精米蛋白质含量的QTL分析[J]. 中国水稻科学, 2012 , 26(3) : 351 -355 . DOI: 10.3969/j.issn.10017216.2012.03.014

Abstract

Based on single seed descend method a recombinant inbred lines  population consisting of 190 lines  was created by crossing two rice varieties with similar apparent amylose content, Nipponbare (japonica) and 9311 (indica) by QTLs for brown rice protein content(BRPC) and milled rice protein content (MRPC) with a genetic linkage map including 202 SSR, CAP and STS markers were identified. By composite interval mapping method in two environments sites (Sanya Hainan Province, China and Hefei Anhui Province, China), a total of nine QTLs were identified, indicating that rice protein content was controlled by multiple genes.  qBRPC1.3 was detected at the two sites with h