In order to clarify the effects of aeration on root nitrogen metabolism in rice seedling, root growth, the activities of glutamine synthetase (GS), glutamic acidpyruvic acid transaminase (GPT), glutamic acid oxaloacetate transaminase (GOT), the contents of nitrate (NO3N), free amino acids and sugar content in root were investigated under continous aeration treatment in hydroponics with indica hybrid rice combination Guodao 6 and japonica Xiushui 09 as material. The results showed that rice seedlings grown in oxygenation solutions had higher root dry matter, longer root length, stronger root activity and larger root absorption area, compared with the control. In addition, the contents of nitrate, soluble sugar, root vigor, the activities of GS, GOT and GPT of aerationtreated rice plants were higher than those of the control. The activities of enzymes involved in root nitrogen metabolism of “Xiushui 09” were enhanced by oxygenation. However, oxygenation had no significant influence on root nitrogen metabolism of Guodao 6, which suggested that oxygenation effect on rice root nitrogen metabolism might be genotypespecific.
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