水稻淡褐斑叶突变体lbsl1的遗传分析与基因定位

奉保华1,杨杨1,2,施勇烽1,林璐3,陈洁1,黄奇娜1,魏彦林1,2,Hei LEUNG4,吴建利1,*

doi:10.3969/j.issn.10017216.2012.03.007

中国水稻科学 >

2012 , Vol. 26 >Issue 3: 297 - 301

DOI: https://doi.org/10.3969/j.issn.10017216.2012.03.007

研究报告

水稻淡褐斑叶突变体lbsl1的遗传分析与基因定位

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¹中国水稻研究所水稻生物学国家重点实验室/国家水稻改良中心，浙江杭州 310006； ² 杭州师范大学生命与环境科学学院，浙江杭州 310036；³ 安徽师范大学生命科学学院，安徽芜湖 241000； ⁴ 国际水稻研究所, 菲律宾马尼拉DAPO 7777 信箱；

收稿日期: 2011-08-11

修回日期: 2011-10-19

网络出版日期: 2012-05-10

基金资助

水稻生物学国家重点实验室自主课题（ZZKT200801）；国家863计划资助项目（2011AA10A101）；中央级公益性科研院所专项基金资助项目。

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Genetic Analysis and Gene Mapping of a Light Brown Spotted Leaf Mutant in Rice

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¹ State Key Laboratory of Rice Biology/Chinese National Center for Rice Improvement, China National Rice Research Institute, Hangzhou 310006, China; ² College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310036, China; ³ College of Life Sciences, Anhui Normal University, Wuhu 241000， China; ⁴ International Rice Research Institute, DAPO Box 7777 Metro Manila, Philippines;

Received date: 2011-08-11

Revised date: 2011-10-19

Online published: 2012-05-10

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摘要

通过EMS诱变籼稻品种IR64获得一个稳定遗传的淡褐色斑点叶突变体lbsl1（light brown spotted leaf 1）。在自然条件下，突变体播种后10~14 d，叶片上出现淡褐色斑点，随后逐渐扩散至全叶，第1叶至剑叶上均有淡褐色斑，为全生育期性状。斑点性状的表达对株高、生育期、结实率和千粒重等农艺性状具有显著的影响。遗传分析结果表明，该淡褐色斑点叶性状受一个隐性核基因控制。将突变体lbsl1与正常叶色水稻Morobereken杂交构建F2定位群体，利用SSR标记，最终将该淡褐叶基因lbsl1(t)定位在第6染色体短臂上一个约130 kb的区段上。定位的结果和发展的群体为该基因的进一步精细定位和克隆奠定了基础。

关键词： 水稻; 叶色; 斑点叶突变体; 分子标记; 基因定位

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奉保华1,杨杨1,2,施勇烽1,林璐3,陈洁1,黄奇娜1,魏彦林1,2,Hei LEUNG4,吴建利1,* . 水稻淡褐斑叶突变体lbsl1的遗传分析与基因定位[J]. 中国水稻科学, 2012 , 26(3) : 297 -301 . DOI: 10.3969/j.issn.10017216.2012.03.007

Abstract

A light brown spottedleaf mutant of rice was isolated from an EMSinduced IR64 mutant bank. The mutant, designated as lbsl1（light brown spottedleaf 1）, displayed light brown spot in the whole growth period from the first leaf to the flag leaf under natural summer field conditions. Agronomic traits including plant height, growth duration, seedsetting rate and 1000grain weight of the mutant were significantly affected. Genetic analysis showed that the mutation was controlled by a single recessive gene, tentatively termed lbsl1(t), which was mapped to the short arm of chromosome 6. With developed SSR markers, the gene was finally delimited to an interval of 130 kb between markers RM586 and RM588. The lbsl1(t) gene is likely a novel rice spottedleaf gene since no other similar genes have been identified near the chromosomal region. The genetic data and recombination populations provided will facilitate further fine mapping and cloning of the gene.

Key words： rice; leaf color; spottedleaf mutant; molecular marker; gene mapping

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