研究报告

水稻(脱氧)胞苷酸脱氨酶基因的表达特性分析

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  • 1 湖南农业大学 作物基因工程湖南省重点实验室, 湖南 长沙 410128; 2 中国农业科学院 植物保护研究所 植物病虫害生物学国家重点实验室, 北京 100193;

收稿日期: 2011-09-15

  修回日期: 2011-10-27

  网络出版日期: 2012-05-10

基金资助

湖南省自然科学基金资助项目(10JJ3042)。

Expression Analysis of Genes Encoding Deoxycytidine/Cytidine Deaminases in Rice

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  • 1 Hunan Provincial Key Laboratory of Crop Gene Engineering, Hunan Agricultural University, Changsha 410128, China; 2 State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences,  Beijing 100193, China;

Received date: 2011-09-15

  Revised date: 2011-10-27

  Online published: 2012-05-10

摘要

以水稻为材料,系统探讨了水稻中(脱氧)胞苷脱氨酶基因的组织表达特性和胁迫诱导表达特性。实时PCR结果表明,7个编码(脱氧)胞苷脱氨酶的基因在苗期水稻根、茎和叶中的表达具有组织特异性,但这些基因在水稻茎中的表达都低于根和叶中的表达;同时,这些基因胁迫诱导表达特性也不尽相同,只有Os07g0245100的表达同时受到低温、干旱、高盐和病害侵染等多种胁迫的诱导。因此,(脱氧)胞苷酸脱氨酶在水稻中可能通过调节体内嘧啶核苷的水平从而在水稻的环境胁迫应答反应中发挥重要调控作用。

本文引用格式

王学军1 ,康厚祥2 ,刘忠松1,* ,王国梁1,2,* . 水稻(脱氧)胞苷酸脱氨酶基因的表达特性分析[J]. 中国水稻科学, 2012 , 26(3) : 261 -266 . DOI: 10.3969/j.issn.10017216.2012.03.002

Abstract

The phylogenetic relationship among the seven deoxycytidine/cytidine deaminase genes and the expression of these genes in different tissues and under different stresses were analyzed. Realtime PCR analysis showed that the seven genes had different expression characteristics in roots, stems and leaves with relative high expression levels in leaves and low expression levels in rice stems. Furthermore, the expression of most  genes was stressinduced including chillness, drought, salination and disease infection, but only the expression of Os07g0245100 was induced by all four stresses. Our results demonstrated that the deoxycytidine/cytidine deaminase genes might play an important  role in abiotic and biotic responses.

参考文献

\[1\]Kroep J  R, van  Moorsel C J, Veerman G, et al. Role of deoxycytidine kinase (dCK), thymidine kinase 2 (TK2), and deoxycytidine deaminase (dCDA) in the antitumor activity of gemcitabine (dFdC). Adv Exp Med Biol,  1998, 431: 657660.

\[2\]Fritz E L, Papavasiliou F N. Cytidine deaminases: AIDing DNA demethylation? Genes Dev,  2010, 24(19): 2107.

\[3\]Takenaka M, Verbitskiy D, van der Merwe J A, et al. The process of RNA editing in plant mitochondria. Mitochondrion,  2008, 8(1): 3546.

\[4\]Ruf S, Bock R. In vivo analysis of RNA editing in plastids. Methods Mol Biol,  2011, 718: 137150.

\[5\]Salone V, Rüdinger M, Polsakiewicz M, et al. A hypothesis on the identification of the editing enzyme in plant organelles. FEBS Lett, 2007, 581(22): 41324138.

\[6\]Carviel J L, AlDaoud F, Neumann M, et al. Forward and reverse genetics to identify genes involved in the agerelated resistance response in Arabidopsis thaliana. Mol Plant Pathol,  2009, 10(5): 621634.

\[7\]Xu J H, Messing J. Maize haplotype with a helitronamplified cytidine deaminase gene copy. BMC Genet,  2006, 7: 52.

\[8\]FaivreNitschke S E, Grienenberger J M, Gualberto J M. A prokaryotictype cytidine deaminase from Arabidopsis thaliana gene expression and functional characterization. Eur J Biochem,  1999, 263(3): 896903.

\[9\]Katahira R, Ashihara H. Profiles of pyrimidine biosynthesis, salvage and degradation in disks of potato (Solanum tuberosum L.) tubers. Planta, 2002, 215(5): 821828.

\[10\] Gowda M, Venu R C, Li H, et al. Magnaporthe grisea infection triggers RNA variation and antisense transcript expression in rice. Plant Physiol,  2007, 144(1): 524533.

\[11\] Wang D, Pan Y, Zhao X, et al. Genomewide temporalspatial  gene expression profiling of drought responsiveness in rice. BMC Genomics,  2011, 12: 149.

\[12\] Yun K Y, Park M R, Mohanty B, et al. Transcriptional regulatory network triggered by oxidative signals configures the early response mechanisms of japonica rice to chilling stress. BMC Plant Biol,  2010, 10: 16.

\[13\] Kumari S, Sabharwal V P, Kushwaha H R, et al. Transcriptome map for seedling stage specific salinity stress response indicates a specific set of genes as candidate for saline tolerance in Oryza sativa L.Funct Integr Genomics,  2009, 9(1): 109123.

\[14\] Kim D W, Shibato J, Agrawal G K, et al. Gene transcription in the leaves of rice undergoing saltinduced morphological changes (Oryza sativa L.). Mol Cells, 2007, 24(1): 4559.

\[15\] Shi B J, Wang G L. Comparative study of genes expressed from rice fungusresistant and susceptible lines during interactions with Magnaporthe oryzae. Gene,  2008, 427(1/2): 80885.

\[16\] Venu R C, Jia Y, Gowda M, et al. RLSAGE and microarray analysis of the rice transcriptome after Rhizoctonia solani infection. Mol Genet Genom,  2007,  278(4): 421431. 
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