水稻光敏色素相互作用因子基因OsPIL11的表达模式及其在光信号传导中作用的初步分析

梁卫红1,*,李莉1,2,彭威风1,2,刘婧2,杨慧2,谢先芝2,3,*

doi:10.3969/j.issn.10017216.2012.03.001

中国水稻科学 >

2012 , Vol. 26 >Issue 3: 255 - 260

DOI: https://doi.org/10.3969/j.issn.10017216.2012.03.001

研究报告

水稻光敏色素相互作用因子基因OsPIL11的表达模式及其在光信号传导中作用的初步分析

展开

¹ 河南师范大学生命科学学院，河南新乡453007； ²山东省农业科学院高新技术研究中心，山东济南 250100； ³ 农业部黄淮海作物遗传改良与生物技术重点开放实验室，山东济南 250100

收稿日期: 2011-08-29

修回日期: 2011-12-02

网络出版日期: 2012-05-10

基金资助

国家自然科学基金资助项目（30971744, 31171182）；河南省科技创新杰出青年计划资助项目（104100510012）。

收起

Expression Patterns of OsPIL11, a Phytochromeinteracting Factor in Rice, and Preliminary Analysis of Its Roles in Light Signal Transduction

Expand

¹ College of Life Science, Henan Normal University, Xinxiang 453007, China; ² HighTech Research Centre, Shandong Academy of Agricultural Sciences, Jinan 250100, China; ³ Key Laboratory of Crop Genetic Improvement and Biotechnology, Huanghuaihai, Ministry of Agriculture, Jinan 250100, China;

Received date: 2011-08-29

Revised date: 2011-12-02

Online published: 2012-05-10

Fold

摘要

分析了OsPIL11基因(6个潜在的PIF转录因子之一）的表达模式，结果表明OsPIL11在水稻中的表达具有器官特异性，并且受叶片发育、脱落酸、茉莉酮酸和水杨酸调控。为了进一步分析OsPIL11在植物光信号传导中的作用，构建了OsPIL11基因的过量表达载体，并将其转化至烟草。通过比较转基因和野生型烟草植株幼苗在红光和远红光下的光形态建成特征，发现红光下生长的OsPIL11转基因烟草下胚轴较短、真叶和子叶较大；然而在远红光下，转基因和野生型烟草植株的表型没有差异。这些结果表明，OsPIL11参与红光诱导的幼苗去黄化反应，但是不参与远红光诱导的幼苗去黄化反应。

关键词： 水稻; 光敏色素相互作用因子; 转基因烟草; 光信号传导

本文引用格式

梁卫红1,*,李莉1,2,彭威风1,2,刘婧2,杨慧2,谢先芝2,3,* . 水稻光敏色素相互作用因子基因OsPIL11的表达模式及其在光信号传导中作用的初步分析[J]. 中国水稻科学, 2012 , 26(3) : 255 -260 . DOI: 10.3969/j.issn.10017216.2012.03.001

Abstract

The expression patterns of OsPIL11, one of six putative phytochromeinteracting factors(PIFs), were analyzed, revealing that expression of OsPIL11was organspecific and regulated by leaf development, ABA, JA and SA. To further explore the role of OsPIL11in plant light signal transduction, plant expression vector of OsPIL11was constructed and introduced into tobacco(Nicotiana tabacum). In continuous red light(R), OsPIL11overexpressed transgenic lines exhibited shorter hypocotyls and larger cotyledons and leaves compared to wildtype seedlings. While in continuous farred light(FR), either transgenic or wildtype seedlings showed similar phenotypes. These results indicated that OsPIL11was involved in Rinduced deetiolation, but not in FRinduced deetiolation in transgenic tobacco.

Key words： rice; phytochromeinteracting factor; transgenic tobacco; light signal transduction

参考文献

\[1\]Takano M, Inagaki N, Xie X Z, et al. Distinct and cooperative functions of phytochromes A, B, and C in the control of deetiolation and flowering in rice. Plant Cell, 2005, 17(12): 33113325.

\[2\]Takano M, Inagaki N, Xie X Z, et al. Phytochromes are the sole photoreceptors for perceiving red/farred light in rice.Proc Natl Acad Sci USA, 2009, 106(34): 1470514710.

\[3\]Franklin K A, Quail P H. Phytochrome functions in Arabidopsis development. J Exp Bot, 2010, 61(1): 1124.

\[4\]Rockwell N C, Su Y S, Lagarias J C. Phytochrome structure signaling mechanisms. Annu Rev Plant Biol, 2006, 57: 837858.

\[5\]Kevei E, Schafer E, Nagy F. Lightregulated nucleocytoplasmic partitioning of phytochromes. J Exp Bot, 2007, 58(12): 31133124.

\[6\]Leivar P, Quail P H. PIFs: Pivotal components in a cellular signaling hub. Trends Plant Sci, 2011, 16(1): 1928.

\[7\]赵晓玲. 植物中与光敏色素相互作用的因子PIFs. 植物生理学通讯, 2009, 45(6): 531536.

\[8\]Ni M, Tepperman J M, Quail P H. PIF3, a phytochromeinteracting factor necessary for normal photoinduced signal transduction, is a novel basic helixloophelix protein. Cell, 1998, 95(5): 657667.

\[9\]Ni M, Tepperman J M, Quail P H. Binding of phytochrome B to its nuclear signaling partner PIF3 is reversibly induced by light. Nature, 1999, 400(6746): 781784.

\[10\]Zhu Y X, Tepperman J M, Fairchild C D, et al. Phytochrome B binds with greater apparent affinity than phytochrome A to the basic helixloophelix factor PIF3 in a reaction requiring the PAS domain of PIF3. Proc Natl Acad Sci USA, 2000, 97(24): 1341913424.

\[11\]MartinezGarcia J F, Huq E, Quail P H. Direct targeting of light signals to a promoter elementbound transcription factor. Science, 2000, 288(5467): 859863.

\[12\]Huq E, AlSady B, Hudson M, et al. PHYTOCHROMEINTERACTING FACTOR 1 is a critical bHLH regulator of chlorophyll biosynthesis. Science, 2004, 305(5692): 19371941.

\[13\]Castillon A, Shen H, Huq E. Phytochrome interacting factors: Central players in phytochromemediated light signaling networks. Trends Plant Sci, 2007, 12(11): 514521.

\[14\]Nakamura Y, Kato T, Yamashino T, et al. Characterization of a set of phytochromeinteractingfacorlike bHLH proteins in Oryza sativa. Biosci Biotechnol Biochem, 2007, 71(5): 11831191.

\[15\]Hood E E, Gelvin S B, Melchers L S, et al. New Agrobacterium helper plasmids for gene transfer to plants. Transgenic Res, 1993, 2(4): 208218.

\[16\]Horsch R B, Fry J E, Hoffmann N L, et al. A simple general method for transferring genes into plants. Science, 1985, 227(4691): 12291231.

\[17\]Xie X Z, Xue Y J, Zhou J J, et al. Phytochromes regulate SA JA signaling pathways in rice are required for developmentally controlled resistance to Magnaporthe grisea. Mol Plant, 2011, 4(4): 688696.

\[18\]Khanna R, Huq E, Kikis E A, et al. A novel molecular recognition motif necessary for targeting photoactivated phytochrome signaling to specific basic helixloophelix transcription factors. Plant Cell, 2004, 16(11): 30333044.

\[19\]Zimmermann P, HirschHoffmann M, Hennig L, et al. GENEVESTIGATOR. Arabidopsis microarray database analysis toolbox. Plant Physiol, 2004, 136(1): 26212632.

\[20\]Shen H, Zhu J, Castillon A, et al. Lightinduced phosphorylation degradation of the negative regulator PHYTOCHROMEINTERACTING FACTOR1 from Arabidopsis depend upon its direct physical interactions with photoactivated phytochromes. Plant Cell, 2008， 20(6): 15861602.

\[21\]Kim D H, Yamaguchi S, Lim S, et al. SOMNUS, a CCCHType zinc finger protein in Arabidopsis, negatively regulates lightdependent seed germination downstream of PIL5. Plant Cell, 2008, 20(5):12601277.

\[22\]Monte E, Tepperman J M, AlSady B, et al. The phytochromeinteracting transcription factor, PIF3, acts early, selectively, positively in lightinduced chloroplast development. Proc Natl Acad Sci USA, 2004, 101(46): 1609116098.

\[23\]Kim J, Yi H, Choi G, et al. Functional characterization of phytochrome interacting factor 3 in phytochromemediated light signal transduction. Plant Cell, 2003, 15(10): 23992407.

\[24\]Huq E, Quail P H. PIF4, a phytochromeinteracting bHLH factor, functions as a negative regulator of phytochrome B signaling in Arabidopsis. EMBO J, 2002, 21(10): 24412450.

Options

文章导航

模态框（Modal）标题

摘要

本文引用格式

Abstract

参考文献