利用24对SSR引物对云南131个陆稻品种进行遗传多样性分析。共检测到195个等位基因。每个位点的等位基因数平均为8.125个，范围在5(RM55)～13(RM218、RM241)之间；平均表观杂合度为0.0014，平均期望杂合度为0.6545；平均ShannonWeaver指数(I)为1.381；Nei基因多样性指数(H)平均为0.6543，变幅为0.2073(RM235)～0.8689(RM218)。不同地区间陆稻种质资源遗传多样性比较分析表明，滇西南和滇南地区存在丰富的遗传变异，是云南陆稻品种遗传多样性的分布中心。藏缅语族和孟高棉语族所种植的陆稻品种遗传多样性最丰富。AMOVA分析表明陆稻的遗传变异主要存在于地区内品种间(82%)，只有3%遗传变异存在于地区间，品种内的遗传变异占15%。聚类分析显示Nei遗传相似系数为0.22时，云南陆稻品种分为籼粳两个类群，主坐标分析与UPGMA聚类结果基本吻合，并将类群Ⅳ的4个偏籼品种从粳稻类群中重新划归到籼稻类群中，校正了UPGMA聚类的误差，但是不能区分地理组。
Genetic diversity of 131 Yunnan upland rice varieties was analyzed by using 24 SSR markers. The average number of alleles per locus was 8.125 with a range from 5(RM55) to 13(RM218, RM241).Mean observed heterozygosity and mean expected heterozygosity were 0.0014 and 0.6545, respectively. Average ShannonWeaver index (I) was 1.381. The Nei′s genetic diversity index (H) ranged from 0.2073(RM235) to 0.8689(RM218) with an average value of 0.6543. The southwest, south of Yunnan were the distribution center of genetic diversity of upland rice varieties in Yunnan, because the highest genetic variation was found in these areas. The analysis of molecular variance(AMOVA) indicated that 82% of the variation was from differences among varieties within regions, and only 3% was due to differences among regions and 15% within varieties. The upland rice varieties planted by TibetoBurman and the MonKhmer family had the most abundant genetic diversity. Cluster analysis showed those varieties could be divided into indica and japonica at the Nei′s genetic similarity of 0.22, but not by geographical distribution. The results of principal coordinates analysis were basically consistent with the UPGMA cluster, four partial indica varieties from group Ⅳ were reclassified into japonica group, the error of the UPGMA cluster was corrected.
\[1\]Venuprasad R L, Atlin H R. Response to direct selection for grain yield under drought stress in rice. Crop Sci, 2007, 47(1): 285.
\[2\]薛全义, 荆宇, 华玉凡. 略论我国旱稻的生产及发展. 辽宁农业职业技术学院学报, 2002, 4(2): 13.
\[3\]李自超, 刘文欣, 赵笃乐. PEG胁迫下水、陆稻幼苗生长势比较研究. 中国农业大学学报, 2001, 6(3): 1620.
\[4\]叶元林, 水建国, 王安明, 等. 旱稻的特性及其效益分析. 中国稻米, 2003 (6): 2628.
\[5\]李自超, 张洪亮, 曾亚文, 等. 云南稻种资源表型遗传多样性的研究. 作物学报, 2001, 27(6): 832837.
\[6\]曾亚文, 李自超, 申时全, 等. 云南地方稻种的多样性及优异种质研究. 中国水稻科学, 2001, 15(3): 169174.
\[7\]吴芳, 殷寿华, 张远辉, 等. 云南热区传统陆稻品种的形态农艺性状多样性研究. 广西农业生物科学, 2003, 22(4): 244248.
\[8\]Zhang Q, Maroof M A S, Lu T Y, et al. Genetic diversity and differentiation of indica and japonica rice detected by RFLP analysis. Theor Appl Genet, 1992, 83(4): 495499.
\[9\]Yu L X, Nguyen H T. Genetic variation detected with RAPD markers among upland and lowland rice cultivars (Oryza sativa L.). Theor Appl Genet, 1994, 87(6): 668672.
\[10\]Tang A, Shao G N, Jiao G A, et al. Comparative assessment of SSR diversity in aromatic rice germplasm. Hereditas (Beijing), 2009, 31(4): 412419.
\[11\]伍绍云, 金建昌. 云南澜沧县陆稻品种资源多样性和原生境保护. 植物资源与环境学报, 2000, 9(4): 3943.
\[12\]蒋志农. 云南稻作. 昆明: 云南科技出版社, 1995: 222.
\[13\] Zhang H, Sun J, Wang M, et al. Genetic structure and phylogeography of rice landraces in Yunnan, China, revealed by SSR. Genome, 2007, 50(1): 7283.
\[14\]Yeh F V, Yang R, Boyle T. Microsoft Windowbased Freeware for Population Genetic Analysis (Popgene 1.32). Edmonton: Canada University of Alberta and Centre for International Forestry Research, 1999.
\[15\]Meirmans P G. Using the AMOVA framework to estimate a standardized genetic differentiation measure. Evolution, 2006, 60(11): 23992402.
\[16\]Xu S, Chen JIE, Liu L, et al. Proteomics associated with virulence differentiation of Curvularia lunata in maize in China. J Integr Plant Biol, 2007, 49(4): 487496.
\[17\]陈丽晖, 梁晓慧, 李科. 基于陆稻地方品种农家保护决策行为的支持系统研究——以云南省景洪市江头曼咪寨为例. 云南地理环境研究, 2009, 21(2): 715.
\[18\]张立娜, 曹桂兰, 韩龙植. 中国不同地理来源旱稻地方品种的遗传相似性研究. 中国农业科学, 2010, 43(17): 34813488.
\[19\]吕广磊, 蔺忠龙, 白现广, 等. 云南栽培稻种SSR遗传多样性比较. 植物学报, 2009, 44(4): 457463.
\[20\]于萍, 李丽, 吕建珍, 等. 太湖流域粳稻地方品种的微卫星分析. 中国水稻科学, 2009, 23(2): 148152.
\[21\]曾亚文, 李自超. 云南地方稻种籼粳亚种的生态群分类及其地理生态分布. 作物学报, 2001, 27(1): 1520.
\[22\]管彦波. 云南稻作源流史. 北京: 民族出版社， 2005：5.
\[23\] 尹绍亭. 基诺族刀耕火种的民族生态学研究. 农业考古，1988(1)： 318334.
\[24\] 周成清. 云南陆稻栽培. 昆明: 云南科技出版社，2007: 15.
\[25\] 薛付忠，王沽贞，张娜，等. 人类群体遗传结构的图论主坐标分析方法. 山东大学学报: 医学版，2006， 44(2)： 109113.
\[26\] 宋烨，翟衡，姚玉新，等. 苹果加工品种遗传多样性分析. 中国农业科学， 2006， 39(1)：139144.
