根据稻螟赤眼蜂(Trichogramma japonicum)的rDNAITS2序列，利用软件Primer Premier 5.0设计出该寄生蜂的特异引物。通过PCR条件的优化，设计的引物能稳定地扩增出稻螟赤眼蜂的特异性条带，大小为256 bp。应用设计的稻螟赤眼蜂特异引物以及报道的松毛虫赤眼蜂(T. dendrolimi)、玉米螟赤眼蜂(T. ostriniae)和螟黄赤眼蜂(T. chilonis)特异引物对赤眼蜂样品进行PCR扩增分析。结果表明，4对特异引物可从单头蜂稳定地扩增出各自蜂种的目的DNA条带，并且分子鉴定结果与形态学鉴定结果完全一致。
The speciesspecific primer for Trichogramma japonicum Ashmead was designed based on its rDNAITS2 sequence with Primer Premier 5.0. With improvement of the conditions of PCR, a 256bp fragment of T. japonicum could be amplified repeatedly with the specificiallydesigned primer. PCR amplifications of Trichogramma samples were carried out with three pairs of diagnostic primers for T. dendrolimi, T. ostriniae, T. chilonis reported previously, and the designed primer for T. japonicum. The results showed that the specific fragment of each Trichogramma species could be amplified with corresponding specific primers, respectively. Furthermore, the results of molecular identification were consistent with those based on morphological identification of male genitalia.
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