以两种生物型的江都杂草稻为材料，考查了杂草稻的生物学性状并对其进行了SSR分析, 旨在确定江都杂草稻的生物类型、遗传特性以及对栽培稻产量的影响。结果表明：杂草稻可分为两个生物型，一类是矮秆类型，包括JDWRA和JDWRC;一类是高秆类型，包括JDWRB。两种类型的杂草稻均属于籼型杂草稻。JDWRA与JDWRC亲缘关系较近，矮秆类型的杂草稻与高秆类型的杂草稻亲缘关系较远，而与杂交稻不育系亲本珍汕97A的亲缘关系较近。杂草稻与栽培稻的田间竞争实验表明，矮秆类型的杂草稻对栽培稻产量因子的影响更为严重。
To explore biotypes, genetic characteristics of weedy rice in Jiangdu, Jiangsu Province, China, and their genetic effect on yield of cultivated rice, morphological characteristics were detected and cluster analysis was conducted based on SSR markers. The results showed that the weedy rice materials can be divided into two biotypes: One is dwarf, including JDWRA and JDWRC, the other one is tall, including JDWRB; the two biotypes of weedy rice belong to indica, JDWRA was most related to JDWRC, the dwarf type of weedy rice was distantly related to the tall type, and closely related to indica male sterile line Zhenshan 97A. The competition test between weedy rice and cultivated rice indicated that the dwarf type of weedy rice more significantly influenced on yield factors of cultivated rice.
\[1\]Gealy D R, Mitten D H, Rutger J N. Gene flow between red rice (Oryza sativa) and herbicideresistant rice (O. sativa): Implications for weed management. Weed Technol, 2003, 17: 627645.
\[2\]Kwon S L, Smith R J. Interference and duration of red rice (Oryza sativa L.) in rice (Oryza sativa). Weed Sci, 1991, 39: 363368.
\[3\]Baki B B, Chin D V, Mortimer M. Wild and weedy rice in rice ecosystems in Asia. Los Banos: International Rice Research Institute, 2000, 118.
\[4\]Federici M T, Vaughan D, Tomooka N, et al. Analysis of Uruguayan weedy rice genetic diversity using AFLP molecular markers. Electron J Biotechnol, 2001, 4(3): 56.
\[5\]Smith R J. Weed thresholds in southern U.S. rice, Oryza sativa. Weed Technol, 1998, 2: 232241.
\[6\]Fischer A J, Ramirez A. Red rice (O. sativa): Competition studies for management decisions. Int J Pest Manage, 1993, 39: 133138.
\[7\]Watanabe H, Azmi M, Ismail M Z. Ecology of major weeds and their control in direct seeding rice culture of Malaysia. MARD/MADA/JIRCAS collaborative Study Report (199296)， 1996. 202.
\[8\] Kwon S L, Smith R J, Talbert R E. Interference of red rice (Oryza sativa) densities in rice (O. Sativa). Weed Sci, 1991, 39: 169174.
\[9\]杨庆, 马殿荣, 宋冬明, 等. 不同密度杂草稻对栽培稻群体形态特征及产量的影响. 北方水稻, 2008, 38(5): 2831.
\[10\]Vaughan L K,Ottis B V, PrazakHavey A M, et al. Is all red rice found in commercial rice really Oryza sativa. Weed Sci, 2001, 49: 468476.
\[11\]王象坤, 才宏伟, 孙传清, 等. 中国野生稻的原始型及其是否存在籼粳分化的探讨问题. 中国水稻科学, 1994, 8(4): 205210.
\[12\]Cho Y C, Chung T Y, Suh H S. Genetic characteristics of Korean weedy rice (Oryza sativa L.) by RFLP analysis. Euphytica, 1995, 86: 103110.
\[13\]Suh H S. Sato Y I, Morishima H. Genetic characterization of weedy rice (Oryza sativa L.) based on morphophysiology, isozymes and RAPD markers. Theor Appl Genet, 1997, 94: 316321.
\[14\]Ishikawa R N, Toki K, Imai Y I, et al. Origin of weedy rice grown in Bhutan and the force of genetic diversity. Genetic Resour Crop Evol, 2005, 52: 395403.
\[15\]Yu G Q, Bao Y, Shi C H, et al. Genetic diversity and population differentiation of Liaoning weedy rice detected by RAPD and SSR markers. Biochem Genet, 2005, 43: 261270.
\[16\]Cao Q J, Lu B R, Xia H, et al. Genetic diversity and origin of weedy rice (Oryza sativa f. spontanea) populations found in Northeastern China revealed by simple sequence repeat (SSR) markers. Ann Bot, 2006, 98: 12411252.
\[17\]Doyle J J, Doyle J L. Isolation of plant DNA from fresh tissue. Focus, 1990, 12: 13l5.
\[18\]唐启义. DPS数据处理系统. 2版. 北京: 科学出版社, 2010.
\[19\]郭勋斌, 顾克礼, 袁秦. 越冬杂草稻的发生与防治研究. 安徽农业科学, 2005, 33(7): 11801181.
\[20\]蒋荷, 吴竞仑, 王根来, 等. 连云港穞稻研究. 作物品种资源, 1985(2): 47.
\[21\]杨杰, 仲维功, 陈志德, 等. 江苏省扬中杂草稻生物学特性初步研究. 杂草科学, 2007(3): 1315.
\[22\]Zhang J, Nilda R B, Ma K, et al. Genetic diversity and genetic relationship of weedy rice in Taizhou City, Jiangsu Province, China. Rice Sci, 2008, 15(4): 295302.
\[23\]Leopoldo E, Estorninos I R, David R, et al. Growth response of rice (Oryza sativa) and red rice (O. sativa) in a replacement series study. Weed Technol, 2002, 16(2): 401406.
\[24\]吴云艳, 马殿荣, 李金英, 等. 不同株型水稻对杂草稻的生态控制研究. 华北农学报, 2010, 25(1): 147150.
