水稻抽穗期主效QTL qHd8.1的精细定位

周勇,崔国昆,张言周,关成冉,常思源,顾铭洪,梁国华*

doi:10.3969/j.issn.10017216.2012.01.008

中国水稻科学 >

2012 , Vol. 26 >Issue 1: 43 - 48

DOI: https://doi.org/10.3969/j.issn.10017216.2012.01.008

研究报告

水稻抽穗期主效QTL qHd8.1的精细定位

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扬州大学江苏省遗传生理重点实验室/教育部植物功能基因组学重点实验室, 江苏扬州 225009；

收稿日期: 2011-01-08

修回日期: 2011-02-19

网络出版日期: 2012-01-10

基金资助

国家973计划资助项目（2011CB100107）；国家自然科学基金资助项目（31071068）；江苏省属高校自然科学重大基础研究项目（10KJA210059）；扬州大学高层次人才科研启动基金资助项目（20100030）。

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Fine Mapping of a Major QTL qHd8.1 for Heading Date in Rice

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Jiangsu Key Laboratory for Crop Genetics and Physiology/Key Laboratory of Plant Functional Genomics of Ministry of Education, Yangzhou University, Yangzhou 225009, China;

Received date: 2011-01-08

Revised date: 2011-02-19

Online published: 2012-01-10

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摘要

从广陆矮4号/日本晴染色体片段代换系群体中筛选到1个株系C23011。该株系除第8染色体短臂上的一个片段来自广陆矮4号外，背景均与日本晴相同。该株系在自然长日照条件下比轮回亲本日本晴提早抽穗约14.1 d，但在短日照条件下，两者抽穗期无显著差异。利用BC4F2世代中筛选到的一个目标区段杂合但背景基本纯合的单株自交一代后得到的BC4F3分离群体进行遗传分析。在该群体中单株抽穗期呈不连续的双峰分布，迟抽穗和早抽穗单株比例符合3∶1的分离比，表明抽穗期受1 个主效QTL qHd8.1控制。利用BC4F3群体中共1628株早抽穗的单株将qHd8.1精细定位在BAC克隆AP005164上标记S8111和S849之间，物理距离约为59.94 kb，该区间共有7个预测基因。

关键词： 水稻; 抽穗期; 数量性状位点; 精细定位

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周勇,崔国昆,张言周,关成冉,常思源,顾铭洪,梁国华* . 水稻抽穗期主效QTL qHd8.1的精细定位[J]. 中国水稻科学, 2012 , 26(1) : 43 -48 . DOI: 10.3969/j.issn.10017216.2012.01.008

Abstract

C23011, containing a single introgressed segment of chromosome 8, was derived from the CSSL population with Nipponbare as the donor and Guanglu′ai 4 as the recurrent parent. In normal longday conditions, its heading date was 14.1 d shorter than that of Nipponbare. Under shortday conditions, however, no obvious difference in heading date between C23011 and Nipponbare was observed. A segregation population derived from a BC4F2 individual with the heterozygous target segment was used for genetic analysis and gene mapping. The ratio of late heading plants to early heading plants showed a 3∶1 frequency distribution，suggesting that the heading date variation was controlled by a single major QTL, which was named qHd8.1. A physical map was constructed encompassing the qHd8.1 locus and the locus was delimited to a 59.94 kb interval between markers S8111 and S849 on BAC clone AP005164 using 1628 individuals with shorter heading date originated from the BC4F3 populations. There are seven predicted genes within this region.

Key words： rice; heading date; quantitative trait loci; fine mapping

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