抗感吡虫啉褐飞虱种群抑制消减杂交 cDNA文库的构建

张珏锋,何月平,陈建明* ,陈列忠

doi:10.3969/j.issn.10017216.2012.01.005

中国水稻科学 >

2012 , Vol. 26 >Issue 1: 21 - 26

DOI: https://doi.org/10.3969/j.issn.10017216.2012.01.005

研究报告

抗感吡虫啉褐飞虱种群抑制消减杂交 cDNA文库的构建

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浙江省植物有害生物防控重点实验室省部共建国家重点实验室培育基地/浙江省农业科学院植物保护与微生物研究所, 浙江杭州 310021;

收稿日期: 2011-03-16

修回日期: 2011-08-26

网络出版日期: 2012-01-10

基金资助

国家自然科学基金资助项目（30771411）；浙江省自然科学基金资助项目（Y307125）；国家科技支撑计划资助项目（2006BAD08A0403）；浙江省科技创新团队资助项目（2010R50028）。

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Construction of Suppression Subtractive Hybridization cDNA Library of Imidaclopridresistant and Imidaclopridsusceptible Brown Planthopper

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State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control/Institute of Plant Protection and Microbiology Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China;

Received date: 2011-03-16

Revised date: 2011-08-26

Online published: 2012-01-10

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摘要

采用抑制消减杂交（SSH）方法构建了对吡虫啉敏感和抗吡虫啉褐飞虱种群的正反向差减cDNA文库，克隆了受吡虫啉调控的相关基因。结果表明，筛选到的120个阳性克隆代表92个互不重复的单基因，其中17个与动物的已知蛋白基因存在较高的同源性。筛选到的单基因，分别涉及信号传导、生物及非生物胁迫、生殖等。

关键词： 吡虫啉; 褐飞虱；抑制消减杂交；基因克隆

本文引用格式

张珏锋,何月平,陈建明* ,陈列忠 . 抗感吡虫啉褐飞虱种群抑制消减杂交 cDNA文库的构建[J]. 中国水稻科学, 2012 , 26(1) : 21 -26 . DOI: 10.3969/j.issn.10017216.2012.01.005

Abstract

The forward and reverse subtracted cDNA libraries of imidaclopridresistant and imidaclopridsusceptible brown planthopper (Nilaparvata lugens Stl) populations were constructed by suppression subtractive hybridization (SSH), and the relative genes regulated by imidacloprid were cloned. The results showed that 120 positive clones obtained represented 92 different single genes, in which 17 shared higher homology with known protein genes of animals in the GenBank. These single genes involved signal transduction, biotic and abiotic stress, reproduction, and etc.

Key words： imidacloprid; Nilaparvata lugens; suppression subtractive hybridization; gene clone

参考文献

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