粳稻02428&times;02428c重组自交系孕穗期耐冷性QTL分析

谢勇武1,2,3杨树明1，*曾亚文1，*杨涛1普晓英1 杜娟1赵春艳2张明喜4

doi:10.3969/j.issn.10017216.2011.06.018

中国水稻科学 >

2011 , Vol. 25 >Issue 6: 681 - 684

DOI: https://doi.org/10.3969/j.issn.10017216.2011.06.018

研究简报

粳稻02428×02428c重组自交系孕穗期耐冷性QTL分析

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¹云南省农业科学院生物技术与种质资源研究所，云南昆明 650205； ²云南农业大学农学院，云南昆明650201； ³昆明田康科技有限公司，云南昆明650231； ⁴ 云南大学生命科学学院, 云南昆明 650091

收稿日期: 2010-12-29

修回日期: 2011-01-10

网络出版日期: 2011-11-10

基金资助

国家自然科学基金资助项目（31060186）；云南省自然科学基金资助项目（2010CD006）；云南省重点新产品开发计划资助项目（2010BB001）；昆明市创新型试点企业项目(昆科计字10N060204号)。

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QTL Analysis of Cold Tolerance in Japonica Rice Using Recombinant Inbred Lines from 02428×02428c at Booting Stage

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¹Biotechnology and Genetic Resources Institute, Yunnan Academy of Agricultural Sciences, Kunming 650205, China; ² College of Agronomy, Yunnan Agricultural University, Kunming 650201, China; ³ Kunming and Tiankang Science and Technology Limited Company, Kunming 650231, China; ⁴School of Life Science, Yunnan University, Kunming 650091, China;

Received date: 2010-12-29

Revised date: 2011-01-10

Online published: 2011-11-10

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摘要

利用孕穗期弱耐冷性品种02428和强耐冷性品种02428c构建了一个包含336个株系的F5：6重组自交系群体。采用自然低温冷害鉴定法，以实粒数、秕粒数、总粒数和结实率作为孕穗期耐冷性状的表型值，用486对SSR标记初步构建分子连锁图谱，对孕穗期耐冷性进行QTL定位。结果表明，标记RM6092、RM6702、RM5954、RM1095、RM1183、RM7643及RM3411与孕穗期耐冷性状连锁；利用QTLMapper 1.6软件检测到位于第1染色体上的3个耐冷性QTL位点。

关键词： 重组自交系；孕穗期; 耐冷性；微卫星标记；数量性状基因座

本文引用格式

谢勇武1,2,3杨树明1，*曾亚文1，*杨涛1普晓英1 杜娟1赵春艳2张明喜4 . 粳稻02428×02428c重组自交系孕穗期耐冷性QTL分析[J]. 中国水稻科学, 2011 , 25(6) : 681 -684 . DOI: 10.3969/j.issn.10017216.2011.06.018

Abstract

A coldsensitive japonica rice variety 02428 and a coldresistant rice variety 02428c were used as parents to produce an F5:6 population composed of 336 recombinant inbred lines. Numbers of filled grains, unfilled grains, total grains and seedsetting rate were measured as phenotypic values for evaluating cold tolerance at the booting stage. A genetic linkage map was constructed with 486 SSR markers for QTL mapping. The markers RM6092, RM6702, RM5954, RM1095, RM1183, RM7643 and RM3411 were linked to the cold tolerance at the booting stage. Three QTLs controlling cold tolerance at the booting stage were identified on the chromosome 1 with QTLMapper 1.6.

Key words： recombinant inbred line; cold tolerance; booting stage; microsatellite marker; quantitative trait locus

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